SV-HUC-1 Cell Line

Brief Introduction
Product List

General Information
Organism	Homo sapiens, human
Cell Line Description	SV-HUC-1 is an immortalized human urothelial cell line established through Simian Virus 40 (SV40)-mediated transformation of normal urothelial tissue. The cell line was derived from normal ureteral tissue obtained from an 11-year-old male patient. SV-HUC-1 cells grow as a flat, cobblestone-like adherent monolayer. This cell line is globally recognized as a fundamental non-malignant control and host model system in human urologic oncology and toxicology research. It is non-tumorigenic and serves as the parental cell line for various malignant sub-clones induced by chemicals or oncogenes (such as the HRAS-transformed MC-SV-HUC T-2 and SV-HUC/ras lineages). The cell line is widely used to elucidate the multistage pathogenesis of bladder cancer, investigate environmental factors contributing to urinary stone formation, map alternative polyadenylation during differentiation, and evaluate bacterial adhesion mechanisms of uropathogenic Escherichia coli and Mycoplasma strains.
Tissue	Ureter; Uroepithelium / Urothelium
Cell Type	Epithelial cell
Disease	Normal (Non-cancerous tissue origin)
Morphology	Epithelial-like
Gender	Male
Age	11 years
Product Format	Frozen
Growth Mode	Adherent
Biosafety Level	1 (Transformed using SV40 but repeatedly verified negative for the shedding of replication-competent infectious viral particles under normal baselines)
Applications	1. Serve as a non-malignant control model for comparative molecular-level studies against bladder cancer cell lines (e.g., T24, 5637, TCCSUP); 2. Act as a primary substrate for chemical and carcinogen transformation assays to evaluate specific stages of the carcinogenic process; 3. Facilitate the analysis of interactions between uropathogenic bacteria and the host; 4. Enable toxicological and environmental impact screening regarding exposure to urinary tract carcinogens and heavy metals; 5. Assess structural variations, transcriptomic profiles, and dynamic changes in alternative polyadenylation within human urothelial cells.
Shipped In	Dry ice
Storage Temperature	−196°C (Liquid nitrogen vapor phase only)
Characteristics
Tumorigenic	No, non-tumorigenic in standard immunocompromised rodent hosts unless deliberately transformed downstream via additional oncogenes or harsh chemical mutagens.
Transformant	Simian virus 40 (SV40) large T antigen integration.
Karyotype	Aneuploid / Polyploid mixture featuring distinct diploid and tetraploid co-existing population sub-clusters.
Viral Production	Natively negative for the production of infectious SV40 particles as measured by African Green Monkey kidney cell plaque assays. Note: Extreme cellular stressors or chemical exposure could theoretically reactivate integrated elements.
Quality Control	Each lot is standardly authenticated via short tandem repeat (STR) profiling and verified sterile.
Mycoplasma Test	Negative
Culture Conditions and Handling
Subculturing	1. Aspirate and discard the spent culture medium from the culture flask. 2. Briefly rinse the confluent cell monolayer with sterile PBS or a Ca2+/Mg2+-free balanced salt solution to neutralize residual serum components. 3. Add 2.0 to 3.0 mL of 0.25% Trypsin-0.53 mM EDTA solution, ensuring the cell layer is completely covered. 4. Incubate at room temperature or 37°C until the cells begin to round up and detach. Key Tip (to prevent clumping): Do not disturb the cells by tapping, vigorously shaking, or rocking the flask while waiting for detachment, as this can cause irreversible physical clumping. 5. Immediately add 6.0 to 8.0 mL of pre-warmed complete growth medium to the flask and disperse the cells into a single-cell suspension by gently pipetting up and down. 6. Centrifuge the cell suspension at approximately 125 × g (or 300 × g) for 5 minutes, carefully discard the supernatant, gently resuspend the cell pellet in fresh complete medium, and proceed with seeding.
Thawing Protocol Note	SV-HUC-1 cells are highly sensitive to fluctuations in environmental pH during the initial stages of thawing and recovery. Before adding the thawed cell suspension, ensure that the culture vessel containing complete growth medium is equilibrated in a 5% CO2 incubator for at least 15 minutes to stabilize the medium's pH within the physiological range of 7.0 to 7.6.
Medium Renewal	2 to 3 times per week
Subcultivation Ratio	Split optimal, confluent cultures at a standard ratio of 1:2 to 1:5.
Culture Conditions	Atmosphere: Air, 95%; CO2, 5%; Temperature: 37°C
Cryopreservation	90% Complete culture medium + 10% DMSO (or standard commercial Freeze Medium CM-1)

Cat.No.	Product Name	Price
CSC-RR00839	Luciferase Reporter Cell Line - SV-HUC-1	Inquiry
CSC-RR00840	GFP Reporter Cell Line - SV-HUC-1	Inquiry
CSC-RO02572	Cas9 Stable Cell Line - Sv-huc-1	Inquiry

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* For research use only. Not intended for any clinical use.

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