DV-90 Cell Line

Brief Introduction
Product List

General Information
Organism	Homo sapiens, human
Cell Line Description	DV-90 is a human lung adenocarcinoma cell line established in 1990 from malignant pleural effusion collected from a 50-year-old male patient with stage IV disease. The cells grow as an adherent monolayer and retain tumor-forming capacity in immunodeficient mice. DV-90 carries heterozygous KRAS p.Gly13Asp and ERBB3 p.Val104Met variants and displays microsatellite instability. Its authenticated STR profile, near-diploid karyotype, genomic datasets, expression profiles, proteomic measurements, drug-response data, and functional-dependency data support mechanistic and translational studies of advanced lung adenocarcinoma.
Cell Type	Human lung adenocarcinoma epithelial cell
Tissue	Lung
Disease	Lung adenocarcinoma
Tumor Stage	Stage IV
Sample Source	Malignant pleural effusion from metastatic disease
Year Established	1990
Gender	Male
Age	50 years
Population Background	Predominantly European genomic ancestry
Morphology	Epithelial-like cells forming an adherent monolayer
Growth Mode	Adherent
Applications	1. Advanced lung adenocarcinoma biology 2. Pleural metastasis and malignant-effusion research 3. KRAS G13D signaling and pathway-dependency studies 4. ERBB3 signaling and receptor-network studies 5. Microsatellite-instability and DNA-repair research 6. Cell proliferation, apoptosis, migration, and invasion assays 7. Anticancer compound screening and combination studies 8. CRISPR, RNA interference, and functional-genomics screens 9. Transcriptomic, genomic, proteomic, and pharmacogenomic profiling 10. HLA-aware antigen-presentation and immunology research 11. Xenograft tumor-growth and treatment-response studies 12. Comparative studies across non-small cell lung cancer models
Characteristics
KRAS Variant	Heterozygous KRAS c.38G>A, producing p.Gly13Asp (G13D)
ERBB3 Variant	Heterozygous ERBB3 c.310G>A, producing p.Val104Met (V104M)
Microsatellite Status	Microsatellite instable
Karyotype	Near diploid with a modal range of 41–47 chromosomes and approximately 17% polyploid cells; recurrent findings include loss of chromosome Y, gain of chromosome 8, and additional material at 6q27
Tumorigenic	Produces tumors in immunodeficient mice
Mycoplasma Test	Negative
Culture Conditions and Handling
Culture Conditions	37°C in a humidified atmosphere containing 5% CO₂
Culture Surface	Standard tissue-culture-treated vessels for routine monolayer propagation
Recommended Seeding Density	Approximately 2 × 10⁶ cells per 25 cm² growth area for routine propagation
Split Ratio and Frequency	Split confluent cultures at 1:2 to 1:3 every 3-4 days using trypsin–EDTA. Adjust the schedule to actual confluence and recovery of the working stock.
Attachment After Seeding	Cells may require 2–3 days to establish firm attachment after seeding. Avoid premature medium changes or assay treatment during early recovery.
Dissociation Reagent	Trypsin–EDTA
Subculturing	1. Passage healthy cultures at confluence using aseptic technique. 2. Remove the spent medium and rinse the monolayer with calcium- and magnesium-free balanced salt solution. 3. Add enough trypsin–EDTA to cover the cell layer. 4. Monitor cell rounding and detachment microscopically and avoid excessive exposure. 5. Add complete serum-containing medium to stop dissociation. 6. Gently disperse aggregates and collect the cell suspension. 7. Seed fresh vessels at approximately 2 × 10⁶ cells per 25 cm² or at a 1:2 to 1:3 split ratio. 8. Return cultures to 37°C and 5% CO₂. Allow adequate attachment time before disturbing the culture.
Typical Harvest	Approximately 10 × 10⁶ cells from an 80 cm² growth area under the reference propagation conditions
Cryopreservation Medium	70% complete culture medium, 20% fetal bovine serum, and 10% DMSO
Cryopreservation	Harvest an actively growing culture and prepare a uniform viable-cell suspension. Resuspend the cells in chilled cryopreservation medium, dispense into labeled cryovials, cool at a controlled rate, and transfer to liquid nitrogen for long-term storage.
Thawing	Rapidly thaw the vial at 37°C, disinfect the exterior, dilute the suspension with pre-warmed complete medium, and remove residual cryoprotectant according to the local recovery workflow. Seed into a suitable vessel and allow 2–3 days for stable attachment before evaluating routine growth.

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* For research use only. Not intended for any clinical use.

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