INS-1 Cell Line

Brief Introduction
Product List

General Information
Organism	Rattus norvegicus, rat
Cell Line Description	INS-1 is a highly reactive rat pancreatic beta-cell line established by C.B. Wollheim and his colleagues in 1992. This cell line was isolated from an in situ pancreatic tumor in an adult male New England Deaconess Hospital (NEDH) strain rat, a tumor that had been experimentally induced via X-ray irradiation. INS-1 cells exhibit an epithelial-like morphology and grow as adherent monolayers. In the field of diabetes research, this cell line is globally recognized as one of the most physiologically relevant in vitro models available. Unlike many other beta-cell lines that tend to lose their differentiated characteristics during continuous passaging, INS-1 cells not only retain high levels of intracellular insulin content but also demonstrate robust and stable glucose-stimulated insulin secretion (GSIS) within the normal physiological range. Consequently, it has become an indispensable tool for investigating insulin biosynthesis, the mechanisms of beta-cell stimulus-secretion coupling, and pathways associated with glucotoxicity and lipotoxicity.
Tissue	Pancreas; Islets of Langerhans
Cell Type	Pancreatic Beta Cell
Disease	Rat Insulinoma
Morphology	Epithelial-like
Gender	Male
Age	Adult
Product Format	Frozen
Growth Mode	Adherent
Biosafety Level	1 (Biosafety classification is based on U.S. Public Health Service Guidelines)
Applications	1. The "gold standard" model for investigating the mechanisms of glucose-stimulated insulin secretion (GSIS) 2. Dissecting the molecular pathways underlying β-cell dysfunction driven by glucotoxicity, lipotoxicity, and endoplasmic reticulum (ER) stress 3. Preclinical screening of novel anti-diabetic drugs, insulin secretagogues, and protective agents 4. Investigating the transcriptional regulatory mechanisms of the insulin gene and β-cell-specific markers 5. Evaluating the mechanisms of cytokine-induced β-cell apoptosis in the construction of Type 1 diabetes models
Shipped In	Dry ice
Storage Temperature	−196°C (Liquid nitrogen vapor phase)
Characteristics
Tumorigenic	Yes, forms insulin-secreting tumors when transplanted into syngeneic or immunocompromised rodents
Secretory Profile	Insulin-positive (capable of synthesizing rat insulin I and II); maintains stable glucose responsiveness within the physiological threshold range (3 mM to 20 mM glucose).
Genetic Profile	They express key beta-cell markers, including PDX-1, GLUT-2, and glucokinase (GCK). Under culture conditions strictly supplemented with thiols, their differentiated function can be stably maintained up to the 60th to 70th generation.
Growth Kinetics	Moderate adherent expansion; typical doubling time spans 36 to 48 hours. Cells grow in characteristic compact epithelial islands.
Mycoplasma Test	Negative
Culture Conditions and Handling
Subculturing	1. Remove and discard the spent culture medium from the culture dish. 2. Gently rinse the cell monolayer with Ca²⁺/Mg²⁺-free Dulbecco's Phosphate-Buffered Saline (DPBS) to neutralize any residual traces of serum containing trypsin inhibitors. 3. Add 1.5 to 2.5 mL of 0.25% Trypsin–0.53 mM EDTA solution, ensuring the cell layer is completely submerged. 4. Incubate at 37°C for 3 to 6 minutes; observe closely under an inverted microscope until the cell islands detach and round up. 5. Add an equal or greater volume of complete culture medium to the dish to thoroughly neutralize the trypsin activity. 6. Centrifuge at approximately 200 × g for 5 minutes; discard the supernatant, uniformly resuspend the cell pellet in fresh medium, and aliquot into new culture dishes.
Medium Renewal	2 to 3 times per week
Subcultivation Ratio	A split ratio of 1:3 to 1:5 is standardly recommended for routine maintenance.
Culture Conditions	Atmosphere: Air, 95%; CO2, 5%; Temperature: 37°C
Cryopreservation	90% Complete growth medium + 10% DMSO (or 50% Basal medium + 40% FBS + 10% DMSO)

Cat.No.	Product Name	Price
CSC-RO02604	Cas9 Stable Cell Line - INS-1	Inquiry
CSC-RR00922	GFP Reporter Cell Line - INS-1	Inquiry
CSC-RR00962	Luciferase Reporter Cell Line - INS-1	Inquiry

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* For research use only. Not intended for any clinical use.

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