SN4741 Cell Line

Brief Introduction
Product List

General Information
Organism	Mus musculus, mouse
Cell Line Description	SN4741 is a conditionally immortalized mouse neural progenitor cell line established by J. H. Son and colleagues in 1999. This cell line was isolated from the substantia nigra (SN) tissue of wild-type mouse embryos at embryonic day 13.5 (E13.5). To achieve conditional immortalization, the researchers genetically engineered these embryonic cells to express a mutant form of the SV40 large T antigen (tsA58) oncogene, which possesses temperature-sensitive properties. This system enables the cells to divide rapidly at lower temperatures, while undergoing growth arrest and morphological changes when transferred to a higher, non-permissive temperature environment. Recognized as a vital in vitro research tool in the field of neuroscience, the SN4741 cell line serves as a foundational model for investigating neurotoxic mechanisms associated with Parkinson's disease (PD), mitochondrial fragmentation processes, and cellular signaling pathways.
Tissue	Brain; Substantia nigra (SN)
Cell Type	Neural Precursor Cell / Immature Dopaminergic Neuron
Disease	Non-tumor (Derived from normal embryonic brain tissue)
Morphology	Fibroblast-like flat morphology with minor processes (at 33°C); transforms to an arborized neuronal morphology with long neurite growth upon differentiation (at 39°C).
Gender	Female (established via cytogenetic mapping of sex chromosomes)
Age	Embryonic Day 13.5 (E13.5)
Product Format	Frozen
Growth Mode	Adherent
Biosafety Level	1 (Contains a non-infectious, integrated temperature-sensitive SV40 large T antigen mutant)
Applications	1. In vitro screening and modeling of neurotoxic cascades associated with Parkinson's disease; 2. Investigation of mitochondrial fragmentation, dynamics, and membrane potential under conditions of chemical insult (e.g., rotenone exposure); 3. Elucidation of transcriptional regulatory and epigenetic modification mechanisms linked to Parkinson's disease susceptibility genes (e.g., DJ-1); 4. Assessment of the effects of neuroimmunomodulators (such as ghrelin) or cellular responses to lipopolysaccharide (LPS) stimulation; 5. Investigation of signaling pathways involved in cell cycle exit and early neuronal differentiation.
Shipped In	Dry ice
Storage Temperature	−196°C (Liquid nitrogen vapor phase)
Characteristics
Tumorigenic	No, non-tumorigenic
Karyotype	Unstable polyploid/triploid; it exhibits complex numerical abnormalities and unbalanced structural rearrangements. Its near-triploid distribution encompasses autosomes 1 through 19, as well as the female sex chromosomes.
Growth States	1. Permissive Conditions (33°C to 37°C): Active proliferation driven by the stable expression of the SV40 tsA58 Large T antigen. 2. Restrictive Conditions (39°C): Heat induction leads to the inactivation of the SV40 Large T antigen, thereby triggering a cell cycle arrest at the G0/G1 phase, downregulating proliferation markers (such as Mki67), and upregulating genes associated with immature neurons.
Lineage Markers	Under permissive temperature conditions, the cells express neural stem cell markers—such as Nestin (Nes)—as well as cell proliferation markers. They exhibit positive immunoreactivity for neuronal markers, including βIII-tubulin (TuJ1) and NeuN, as well as for dopaminergic markers such as Tyrosine Hydroxylase (TH), DAT, Foxa2, and Nr4a2. They test negative for the glial cell marker GFAP.
Growth Kinetics	Proliferates at the permissive temperature with an approximate doubling time of 36 hours.
Mycoplasma Test	Negative
Culture Conditions and Handling
Subculturing (Expansion Only)	1. Remove and discard the spent complete culture medium from the culture flask. 2. Gently rinse the adherent cell layer with sterile, Ca²⁺/Mg²⁺-free PBS to remove residual traces of serum. 3. Add 1.0 to 2.0 mL of 0.25% Trypsin–0.53 mM EDTA solution, ensuring the cell monolayer is completely submerged. 4. Incubate at the standard growth temperature for approximately 1 minute; observe closely under an inverted microscope until the cells round up and detach. 5. Add an equal volume of pre-warmed complete culture medium to rapidly neutralize the enzymatic activity. 6. Centrifuge the cell suspension at 1100 rpm for 4 minutes; aspirate the supernatant, resuspend the cell pellet in fresh medium, and seed into a new culture dish. Note: Cell expansion and maintenance procedures must be strictly performed within the temperature range of 33°C to 37°C (permissible conditions).
Differentiation Induction	To induce growth arrest and transition into differentiated, mature neuronal phenotypes, seed the cells into experimental plates using complete growth medium, transfer them into an incubator set strictly to 39°C (restrictive temperature), and reduce the serum concentration for 48 hours without subculturing.
Subcultivation Ratio	A split ratio of 1:2 to 1:3 is recommended during the routine proliferative phase. Passage cells actively when density reaches 80% confluence.
Culture Conditions	Atmosphere: Air, 95%; CO2, 5%; Temperature: 33°C to 37°C for expansion, 39°C for differentiation.
Cryopreservation	75% Complete culture medium + 20% FBS + 5% DMSO (or 90% FBS + 10% DMSO)

Cat.No.	Product Name	Price
CSC-RO02579	Cas9 Stable Cell Line - SN4741	Inquiry
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CSC-RR00931	Luciferase Reporter Cell Line - SN4741	Inquiry

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* For research use only. Not intended for any clinical use.

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