Calu-1 Cell Line

Brief Introduction
Product List

General Information
Organism	Homo sapiens, human
Cell Line Description	Calu-1 is a human non-small cell lung cancer (NSCLC) cell line established in 1971 by Jørgen Fogh at the Memorial Sloan Kettering Cancer Center. The cell line was isolated from the metastatic pleural effusion of a 47-year-old Caucasian male patient diagnosed with Grade III pulmonary epidermoid carcinoma (squamous cell carcinoma). Calu-1 cells are polygonal, exhibit an epithelioid morphology, and grow as a robust adherent monolayer. In the field of translational oncology, Calu-1 is recognized as a pivotal and highly aggressive model of KRAS-mutant lung squamous cell carcinoma. It serves as a vital platform for evaluating covalent KRAS inhibitors, investigating epithelial-mesenchymal transition (EMT), elucidating behavioral patterns ranging from collective migration to single-cell invasion, and analyzing the metastatic progression of thoracic malignancies.
Tissue	Lung; derived from metastatic site (Pleural Effusion)
Cell Type	Epithelial cell
Population / Ancestry	Caucasian
Disease	Lung Squamous Cell Carcinoma (Epidermoid Carcinoma) / Non-Small Cell Lung Cancer (NSCLC)
Morphology	Epithelial-like
Gender	Male
Age	47 years
Product Format	Frozen
Growth Mode	Adherent
Biosafety Level	1 (Biosafety classification aligns with standard international repository guidelines for non-infectious human cancer lines)
Applications	1. "Gold-standard" in vitro and in vivo models for studying biological networks driven by the KRAS G12C oncogene and evaluating selective covalent G12C inhibitors; 2. Elucidation of signaling pathways involving non-receptor tyrosine kinases, the Hippo signaling cascade, and cell-matrix interactions during metastatic colonization; 3. Standard models utilizing 3D co-culture matrices to study epithelial-mesenchymal transition (EMT) dynamics and the transition from collective to single-cell invasion; 4. Preclinical screening of novel combination therapy regimens involving targeted therapies, radiosensitizers, and microtubule stabilizers; 5. Establishment of subcutaneous, orthotopic, or experimental lung cancer metastasis models in immunodeficient mice.
Shipped In	Dry ice
Storage Temperature	−196°C (Liquid nitrogen vapor phase only)
Characteristics
Tumorigenic	Yes, highly tumorigenic; readily forms progressively growing, invasive epidermoid carcinomas when engrafted subcutaneously or orthotopically into athymic nude rodents.
Transformant	None; immortalized spontaneously from advanced metastatic clinical tumor tissue.
Oncogenic Driver	KRAS p.Gly12Cys (G12C). Harbors a classic, homozygous oncogenic activating mutation (c.34G>T) in the KRAS gene, driving ligand-independent GTPase activation and signaling.
Karyotype & Genetics	Human hypotriploid karyotype with a modal chromosome number of 62.
Mycoplasma Test	Negative
Culture Conditions and Handling
Subculturing	1. Remove and discard all used complete growth medium from the culture vessel. 2. Rinse the cell monolayer with sterile Ca2+/Mg2+-free PBS buffer to neutralize any residual serum containing active trypsin inhibitors. 3. Add 1.0 to 2.0 mL of pre-warmed 0.25% trypsin–0.53 mM (0.03%) EDTA solution, ensuring the cell layer is completely submerged. 4. Incubate at room temperature (or 37°C) for 3 to 7 minutes. Monitor under an inverted microscope until the cells round up, loosen, and detach completely. 5. Immediately add 6.0 to 8.0 mL of pre-warmed complete growth medium to neutralize enzyme activity. 6. Gently pipette the suspension to disperse cell clumps, centrifuge at ~125 × g for 5 to 7 minutes, discard the supernatant, gently resuspend the cells in fresh complete growth medium, and distribute into new culture vessels.
Thawing Protocol Note	Calu-1 cells are sensitive to pH and osmotic shocks during the early recovery phase following thawing. Before adding the thawed cell suspension, the culture vessel containing fresh complete growth medium should be placed in a 5% CO2 incubator for at least 15 minutes to allow the medium to equilibrate to a physiological pH range (7.0 to 7.6).
Medium Renewal	2 to 3 times per week
Subcultivation Ratio	Split confluent cultures (80% density) at a standard ratio of 1:2 to 1:4 every 6 to 8 days.
Culture Conditions	Atmosphere: Air, 95%; CO2, 5%; Temperature: 37°C
Cryopreservation	90% Complete culture growth medium + 10% DMSO (or 70% Basal medium + 20% FBS + 10% DMSO)

Cat.No.	Product Name	Price
CSC-RT2639	Human ICAM1 Knockout Cell Line-Calu-1	Inquiry
CSC-RR01009	GFP/Luc Reporter Cell Line - Calu-1	Inquiry

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* For research use only. Not intended for any clinical use.

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