NCI-H1650 Cell Line

Brief Introduction
Product List

General Information
Organism	Homo sapiens, human
Cell Line Description	NCI-H1650 is a human non-small cell lung cancer (NSCLC) cell line derived from a stage 3B bronchioloalveolar carcinoma (lung adenocarcinoma) specimen obtained from a 27-year-old male patient. These cells exhibit an epithelial-like morphology, grow as an adherent monolayer under standard culture conditions, and retain characteristics typical of lung adenocarcinoma. Cytogenetically, the cell line displays an aneuploid karyotype characterized by complex numerical and structural chromosomal alterations. At the molecular level, NCI-H1650 harbors an in-frame deletion mutation in exon 19 of the EGFR gene (delE746-A750) and a homozygous deletion of the tumor suppressor gene PTEN, while expressing wild-type KRAS. Due to these distinct genetic features—specifically the EGFR mutation and PTEN deletion—NCI-H1650 has become a vital in vitro model for investigating the pathogenesis of lung adenocarcinoma, exploring mechanisms of resistance to EGFR tyrosine kinase inhibitors (TKIs), evaluating targeted therapies, and conducting high-throughput functional genomic screens.
Tissue	Lung
Disease	Bronchioalveolar Carcinoma; Lung Adenocarcinoma; Non-Small Cell Lung Cancer (NSCLC)
Morphology	Epithelial
Gender	Male
Age	27 years
Product Format	Frozen
Growth Mode	Adherent
Biosafety Level	1 (Biosafety classification is based on U.S. Public Health Service Guidelines)
Applications	1. Research on the biology and oncogenic signaling pathways of non-small cell lung cancer (NSCLC); 2. Research on the mechanisms of primary and acquired resistance to EGFR tyrosine kinase inhibitors (TKIs); 3. Research on PTEN loss and PI3K/Akt pathway activation in lung cancer; 4. High-throughput screening of candidate drugs, evaluation of targeted therapies, and functional genomics research.
Shipped In	Dry ice
Storage Temperature	−196°C
Characteristics
Karyotype	Aneuploid
Tumorigenic	Yes, tumorigenic in immunocompromised (nude) mice
Genetic Profile	EGFR exon 19 deletion (delE746-A750); PTEN homozygous deletion; TP53 mutation; KRAS wild-type
Expression Markers	Expresses mutant EGFR; positive for epithelial markers (Cytokeratins)
Mycoplasma Test	Negative
Culture Conditions and Handling
Subculturing	1. Remove and discard the culture medium. 2. Briefly rinse the cell monolayer with PBS (without Ca2+/Mg2+) to completely remove residual serum. 3. Add 1.0 to 2.0 mL of 0.25% trypsin-0.03% EDTA solution and incubate at 37°C until the cell monolayer detaches (typically 2 to 5 minutes). 4. Add fresh complete growth medium to neutralize the trypsin and gently pipette up and down to prepare a single-cell suspension. 5. Subculture the cell suspension into new culture vessels.
Medium Renewal	Every 2 to 3 days
Subcultivation Ratio	A split ratio of 1:3 to 1:6 is recommended
Culture Conditions	Atmosphere: Air, 95%; CO2, 5%; Temperature: 37°C
Cryopreservation	Complete growth medium supplemented with 5% to 10% (v/v) DMSO

Cat.No.	Product Name	Price
CSC-RR00998	GFP/Luc Reporter Cell Line - NCI-H1650	Inquiry

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* For research use only. Not intended for any clinical use.

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