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| General Information | |
| Organism | Mus musculus, mouse |
| Cell Line Description | In 1954, Dr. Lloyd Law successfully isolated the L1210 cell line by exposing DBA/2 mice to the chemical carcinogen 3-methylcholanthrene. This cell line was derived from the ascites fluid of an 8-month-old female DBA/2 mouse suffering from lymphocytic leukemia. The specific methodology involved applying methylcholanthrene to the mice's skin to induce tumor formation, thereby enabling researchers to investigate the process of cancer development in depth. While the L1210 cells are fundamentally B lymphocytes, they morphologically exhibit the characteristics of lymphoblasts. This cell line is renowned for its exceptionally rapid growth rate and a nearly 100% "take rate" (i.e., successful engraftment rate) within syngeneic hosts. The vigorous growth and extraordinary proliferative capacity of L1210 cells make them an ideal model for studying the pathogenesis of leukemia. Furthermore, L1210 cells grow rapidly in in vitro culture environments and are capable of being maintained in suspension culture. This characteristic renders them an excellent resource for conducting both in vivo experiments and in vitro assays. |
| Tissue | Peripheral blood; Ascites |
| Disease | Lymphocytic Leukemia; Lymphoma |
| Morphology | Lymphoblast-like; Round, individual cells |
| Gender | Female |
| Age | 8 months |
| Product Format | Frozen |
| Growth Mode | Suspension |
| Biosafety Level | 1 (Biosafety classification is based on U.S. Public Health Service Guidelines) |
| Applications | 1. Preliminary screening and efficacy assessment of novel anti-leukemia drugs 2. Investigation into the biochemical mechanisms of drug resistance 3. Establishment of syngeneic ascites or solid tumor models using DBA/2 mice 4. High-Throughput cytotoxicity assays and cell viability studies 5. Analysis of nucleoside transport and metabolic pathways |
| Shipped In | Dry ice |
| Storage Temperature | −196°C |
| Characteristics | |
| Tumorigenic | Yes, highly tumorigenic in syngeneic DBA/2 mice |
| Karyotype | Aneuploid; modal number = 38-40; contains characteristic marker chromosomes (e.g., t(12;15)) |
| Growth Kinetics | Extremely rapid; doubling time typically ranges between 10 to 12 hours |
| Phenotype | Expresses B-lymphocytic lineage markers; high sensitivity to many chemotherapeutic drugs |
| Mycoplasma Test | Negative |
| Culture Conditions and Handling | |
| Subculturing | 1. Suspension cells do not require enzymatic digestion. 2. Collect the cell suspension by centrifugation (e.g., 1000 rpm for 5 minutes). 3. Discard the supernatant and resuspend the cell pellet in fresh culture medium. 4. Aliquot the cells into new culture vessels according to the desired subculturing ratio. 5. It is recommended to maintain the cell density between 1 x 10^5 and 1 x 10^6 cells/mL. |
| Medium Renewal | Every 2 to 3 days |
| Subcultivation Ratio | 1:10 to 1:20 (due to its extremely high proliferative rate) |
| Culture Conditions | Atmosphere: Air, 95%; CO2, 5%; Temperature: 37°C |
| Cryopreservation | 90% Complete growth medium + 10% DMSO |
| Cat.No. | Product Name | Price |
|---|---|---|
| CSC-RR00789 | Luciferase Reporter Cell Line - L1210 | Inquiry |
| CSC-RO01503 | OVAL Stable Cell Line - L1210 | Inquiry |
| CSC-RO01756 | GFP/OVAL Stable Cell Line - L1210 | Inquiry |
| CSC-RO02009 | Luc/OVAL Stable Cell Line - L1210 | Inquiry |
| CSC-RO02262 | GFP/Luc/OVAL Stable Cell Line - L1210 | Inquiry |
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