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| General Information | |
| Organism | Homo sapiens, human |
| Cell Line Description | C666-1 is a human nasopharyngeal carcinoma (NPC) cell line established in 1999 by Siu-Tim Cheung, Dolly P. Huang, and their colleagues at the Chinese University of Hong Kong. It is a subclone of the parental C666 cell line, which was originally derived from an undifferentiated NPC xenograft originating in Southern China. C666-1 is globally recognized as the only naturally EBV-infected epithelial cell model capable of stably and persistently harboring the Epstein-Barr virus (EBV) genome in in vitro culture. These cells exhibit a typical polygonal epithelial morphology, grow as an adherent monolayer, and lack contact inhibition. In the fields of translational oncology and virology, C666-1 serves as a pivotal model for studying host-pathogen interactions, mechanisms of viral oncogenesis, and the dynamics of EBV latent infection. |
| Tissue | Head and Neck; derived from Nasopharynx |
| Cell Type | Epithelial cell |
| Disease | Undifferentiated Nasopharyngeal Carcinoma (NPC) |
| Morphology | Epithelial-like; uniform polygonal cells |
| Gender | Male |
| Age | Unspecified |
| Product Format | Frozen |
| Growth Mode | Adherent |
| Biosafety Level | 2 (BSL-2 is required globally because the cell line consistently harbors and replicates the episomal Epstein-Barr virus genome) |
| Applications | 1. A unique in vitro model serving as the "gold standard" for studying the biological characteristics of natural latent Epstein-Barr virus (EBV) infection in epithelial cells. 2. In-depth analysis of the expression, transcriptional regulatory mechanisms, and oncogenic pathways of EBV-encoded latent genes (EBNA1, LMP1, LMP2) and non-coding viral RNAs. 3. In-depth analysis of signaling and transcriptional regulatory networks driven by EBV-encoded microRNAs (such as members of the BART family, including miR-BART21 and miR-BART22). 4. Preclinical screening of novel anti-tumor therapies, radiosensitizers, and EBV-targeted molecular inhibitors. 5. Investigation of cancer stem-like cells (CSCs), sphere-forming capacity, and cell surface markers (such as CD44) in EBV-associated nasopharyngeal malignancies. |
| Shipped In | Dry ice |
| Storage Temperature | −196°C |
| Characteristics | |
| Tumorigenic | Yes, highly tumorigenic; readily forms progressively growing tumors when subcutaneously or orthotopically engrafted into athymic nude mice. |
| Transformant | Epstein-Barr virus (EBV). |
| Karyotype | Sub-diploid / Hypodiploid; displays a highly stable chromosomal modal number of 45. |
| EBV Latency Profile | They express EBV-encoded RNAs (EBERs), the EBNA1 protein, and LMP1/LMP2 transcripts, exhibiting a typical EBV latency II pattern that mimics primary human tumors. The endogenous viral genotype is EBV-1, characterized by a specific 30-bp deletion in the carboxyl-terminus of the latent membrane protein 1 (LMP1) gene—a feature highly consistent with primary nasopharyngeal carcinoma (NPC) biopsy samples from patients in southern China. |
| Phenotypic Markers | Positively expresses epithelial markers, including cytokeratins. |
| Growth Kinetics | Relatively slow adherent proliferation; average population doubling time is approximately 3.5 days (84 hours). |
| Mycoplasma Test | Negative |
| Culture Conditions and Handling | |
| Subculturing | 1. Remove and discard all used complete growth medium from the culture vessel. 2. Gently rinse the cell monolayer with sterile, Ca2+/Mg2+-free PBS to neutralize any residual traces of serum containing active protease inhibitors. 3. Add 1.0 to 2.5 mL of pre-warmed 0.25% trypsin-0.53 mM (0.03%) EDTA solution, ensuring the cells are completely submerged. 4. Incubate at 37°C for 3 to 8 minutes; monitor under an inverted microscope until the cells become rounded, loosen, and fully detach. 5. Immediately add an equal volume of complete growth medium to neutralize trypsin activity. 6. Gently pipette the suspension to disperse cell clumps, centrifuge at ~125 × g for 5 minutes, discard the supernatant, gently resuspend the cell pellet in fresh complete growth medium, and distribute into new culture vessels. |
| Medium Renewal | 2 to 3 times per week |
| Subcultivation Ratio | Split confluent cultures at a standard ratio of 1:2 to 1:4. Avoid over-diluting the cells, as they expand most efficiently when maintained at moderate density. |
| Culture Conditions | Atmosphere: Air, 95%; CO2, 5%; Temperature: 37°C |
| Cryopreservation | 90% Complete culture growth medium + 10% DMSO (or 50% RPMI 1640 + 40% FBS + 10% DMSO) |
| Cat.No. | Product Name | Price |
|---|---|---|
| CSC-RR00981 | GFP/Luc Reporter Cell Line - C666-1 | Inquiry |
| CSC-RR01183 | Luciferase Reporter Cell Line - C666-1 | Inquiry |
| CSC-RR01184 | GFP Reporter Cell Line - C666-1 | Inquiry |
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