AML-12 Cell Line

Brief Introduction
Product List

General Information
Organism	Mus musculus, mouse
Cell Line Description	AML-12 (Alpha Mouse Liver 12) is a non-malignant, immortalized mouse hepatocyte cell line established in 1994. This cell line was derived from the liver of a CD-1 (ICR) transgenic mouse carrying a human transforming growth factor-alpha (TGF-α) transgene driven by the mouse albumin promoter (Tg(Alb-1TGFa)22Bri). AML-12 cells grow as an adherent monolayer and exhibit the typical polygonal, epithelial-like morphology of hepatocytes. In the fields of hepatology, toxicology, and metabolic research, AML-12 is recognized as a high-quality, "gold standard" model of non-tumorigenic, functional mouse hepatocytes. Unlike hepatocellular carcinoma cell lines, AML-12 cells stably maintain differentiated hepatocyte functions, including serum protein synthesis, peroxisome proliferation, active glycogen storage, and inducible cytochrome P450 enzyme activity.
Tissue	Liver
Cell Type	Hepatocyte (Parenchymal Liver Cell)
Strain	CD-1 (ICR) transgenic
Disease	Normal / Transgenic Differentiated Hepatocyte Model
Morphology	Epithelial-like; polygonal hepatocytes with granular cytoplasm and binucleation
Gender	Male
Age	Unspecified adult transgenic mouse
Product Format	Frozen
Growth Mode	Adherent
Biosafety Level	1 (Biosafety classification matches standard laboratory safety parameters for non-infectious rodent primary-like lines)
Applications	1. A "gold standard" non-tumorigenic in vitro model for studying hepatic lipid metabolism, fatty liver disease (NAFLD/MASLD), and lipotoxicity; 2. Screening for drug-induced liver injury (DILI), hepatotoxicity, and PPARα agonists; 3. Elucidating mechanisms of insulin signaling pathways, hepatic gluconeogenesis, glycogen synthesis, and glucose output; 4. Investigating drug biotransformation, cytochrome P450 (CYP450) induction, and Phase II detoxification enzymes; 5. Establishing models for acute hepatic stress, ischemia-reperfusion injury, and the dynamic processes of liver regeneration.
Shipped In	Dry ice
Storage Temperature	−196°C
Characteristics
Tumorigenic	No, non-tumorigenic under standard physiological baselines; displays contact inhibition and typical non-malignant hepatocyte morphology.
Transformant	Human transforming growth factor-alpha (TGF-α) driven by the mouse albumin promoter (Alb-1).
Functional Hepatocyte Markers	Endogenously expresses key liver-specific markers and serum proteins, including albumin, α1-antitrypsin, transferrin, and cytokeratin 18 (CK18).
Ultrastructure & Metabolism	Electron microscopy confirms rich, functional intracellular organelles typical of primary hepatocytes: abundant peroxisomes, rough and smooth endoplasmic reticulum, mitochondria, and glycogen storage granules.
Growth Kinetics	Moderate to rapid adherent expansion; population doubling time spans approximately 24 to 36 hours.
Mycoplasma Test	Negative
Culture Conditions and Handling
Subculturing	1. Completely aspirate and discard the complete culture medium from the culture vessel. 2. Gently rinse the cell monolayer with sterile, Ca2+/Mg2+-free PBS to remove residual serum containing trypsin inhibitors. 3. Add 1.0 to 2.5 mL of 0.25% trypsin-0.53 mM (0.02%) EDTA solution, pre-warmed to the appropriate temperature, ensuring the cell layer is completely covered. 4. Incubate at 37°C for 3 to 7 minutes; monitor under an inverted microscope until the cells round up, loosen, and completely detach. 5. Add an equal volume of pre-warmed complete growth medium to immediately neutralize trypsin activity. 6. Gently pipette the cell suspension to disperse the cells, centrifuge at approximately 125 × g for 5 minutes, carefully discard the supernatant, gently resuspend the cells in fresh complete growth medium, and distribute them into new culture vessels.
Thawing Protocol Note	AML-12 cells are sensitive to pH changes during early post-thaw recovery. Prior to introducing thawed vial contents, place the culture vessel containing fresh complete growth medium into the 5% CO2 incubator for at least 15 minutes to allow the medium to equilibrate to its physiological pH range (7.0 to 7.6).
Medium Renewal	2 to 3 times per week
Subcultivation Ratio	Split confluent cultures (80% density) at a standard ratio of 1:3 to 1:6. Maintain active growth by subculturing before cells become completely overgrown or piled up.
Culture Conditions	Atmosphere: Air, 95%; CO2, 5%; Temperature: 37°C
Cryopreservation	90% Complete culture growth medium + 10% DMSO (or 50% DMEM/F12 + 40% FBS + 10% DMSO)

Cat.No.	Product Name	Price
CSC-RR00864	Luciferase Reporter Cell Line - AML-12	Inquiry
CSC-RR00865	GFP Reporter Cell Line - AML-12	Inquiry

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* For research use only. Not intended for any clinical use.

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