KPC Cell Line

Brief Introduction
Product List

General Information
Organism	Mus musculus, mouse
Cell Line Description	KPC is a syngeneic murine pancreatic ductal adenocarcinoma (PDAC) cell line derived from pancreatic tumors that spontaneously develop in the clinically relevant, genetically engineered KPC mouse model (LSL-Kras(G12D/+); LSL-Trp53(R172H/+); Pdx-1-Cre). In vitro, KPC cells grow as a loosely adherent monolayer and exhibit a characteristic polygonal, epithelial-like morphology. The KPC cell line is regarded as the "gold standard" model for PDAC research in the fields of cancer biology, drug development, and immunobiology. Because the cell line shares a syngeneic genetic background with immunocompetent C57BL/6 mice, it can be transplanted orthotopically or subcutaneously into these animals. This enables researchers to investigate pancreatic tumor biology, the dynamics of the tumor microenvironment (TME), and the efficacy of immune checkpoint immunotherapies within a host possessing a fully functional immune system and normal physiology.
Tissue	Pancreas
Cell Type	Pancreatic Ductal Epithelial Cell (Malignant)
Strain	C57BL/6 (specifically backcrossed onto C57BL/6J)
Disease	Pancreatic Ductal Adenocarcinoma (PDAC)
Morphology	Epithelial-like; polygonal, growing in cohesive monolayer sheets
Gender	Male (The classic cell line cataloged on the pure C57BL/6 background is male-derived)
Growth Mode	Loosely adherent (Monolayer with some loosely attached cell cohorts)
Biosafety Level	1 (Biosafety classification matches standard laboratory parameters for non-infectious, replication-deficient transgenic rodent cell lines)
Applications	1. High-quality syngeneic in vivo models for studying pancreatic cancer tumor-immune microenvironment (TIME) interactions and local immune escape; 2. Preclinical testing of novel small-molecule chemotherapy combinations, targeted KRAS inhibitors, and immunotherapeutic agents (e.g., anti-PD-1, anti-CTLA-4, or CD40 agonists); 3. Elucidation of the molecular, genetic, and metabolic pathways driving acinar-to-ductal metaplasia (ADM), tumor invasion, and spontaneous metastasis; 4. Investigation of stroma-induced drug resistance mechanisms and the dynamic changes of physical barriers (utilizing 3D co-culture systems with hepatic stellate cells or myofibroblasts); 5. Establishment of reproducible orthotopic, subcapsular, or subcutaneous pancreatic tumor models in immunocompetent mice, with precise control over timing and localization.
Shipped In	Dry ice
Storage Temperature	−196°C
Characteristics
Tumorigenic	Yes, highly tumorigenic and metastatic; has a tumor take rate of nearly 100% when engrafted orthotopically or subcutaneously into syngeneic C57BL/6 mice.
Oncogenic Drivers	Dual mutation profile that closely mirrors human PDAC pathogenesis: 1. Kras G12D: Conditionally activated point mutation in the Kras gene, driving continuous down-stream proliferation signaling. 2. Trp53 R172H: Point mutation in the transformation-related protein 53 gene (the murine analog of the human TP53 R175H hot-spot mutation), leading to genomic instability.
Establishment & Genetic Cre-Recombination	This model was generated by directly targeting the mouse pancreas for the dual expression of KrasG12D and Trp53R172H under the control of the pancreas-specific Pdx-1 promoter. The Pdx-1 promoter drives the expression of Cre recombinase, which excises the LoxP-Stop-LoxP (LSL) transcriptional silencing cassette, thereby specifically triggering the selective endogenous expression of these two oncogenes within the pancreatic epithelium.
Growth Kinetics	Robust, rapid adherent expansion; population doubling time is approximately 24 to 48 hours.
Mycoplasma Test	Negative
Culture Conditions and Handling
Subculturing	1. Remove and discard all used complete culture medium from the culture vessel. 2. Gently rinse the cell monolayer with sterile, Ca2+/Mg2+-free PBS to neutralize any residual serum containing active trypsin inhibitors. 3. Add 1.0 to 2.0 mL of pre-warmed 0.25% trypsin–0.53 mM (0.02%) EDTA solution, ensuring the cell layer is completely submerged. 4. Incubate at 37°C for 2 to 5 minutes; monitor under an inverted microscope until the cells become rounded, loosen, and completely detach from the surface. 5. Add an equal volume of pre-warmed complete culture medium to rapidly neutralize enzyme activity. 6. Gently pipette the suspension to disperse cell clumps, centrifuge at approximately 250 × g for 5 minutes, carefully aspirate the supernatant, gently resuspend the pellet in fresh complete growth medium, and distribute into new vessels.
Thawing Protocol Note	KPC cells are relatively sensitive to the initial culture environment after thawing. Before adding the thawed cell suspension, the culture vessel containing fresh complete growth medium should be placed in a 5% CO2 incubator for at least 15 minutes to allow the medium to reach and equilibrate at the normal physiological pH range (7.0 to 7.6).
Medium Renewal	Every 2 days
Subcultivation Ratio	Split confluent cultures (80% density) at a standard ratio of 1:2 to 1:4 every 2 days.
Culture Conditions	Atmosphere: Air, 95%; CO2, 5%; Temperature: 37°C
Cryopreservation	90% Complete culture growth medium + 10% DMSO (or 50% DMEM + 40% FBS + 10% DMSO)

Cat.No.	Product Name	Price
CSC-RR0630	Luciferase Reporter Cell Line - KPC	Inquiry
CSC-RO01611	OVAL Stable Cell Line - KPC	Inquiry
CSC-RO01864	GFP/OVAL Stable Cell Line - KPC	Inquiry
CSC-RO02117	Luc/OVAL Stable Cell Line - KPC	Inquiry
CSC-RO02370	GFP/Luc/OVAL Stable Cell Line - KPC	Inquiry
CSC-RR01202	Nluc Reporter Cell Line - KPC	Inquiry

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