EO771 Cell Line

Brief Introduction
Product List

General Information
Organism	Mus musculus, mouse
Cell Line Description	EO771 is a syngeneic murine mammary adenocarcinoma cell line originally reported in 1948 as a spontaneous mammary carcinoma arising in a C57BL/6 mouse. EO771 cells grow as an adherent monolayer of elongated, epithelial-like cells. In modern oncology, tumor immunology, and preclinical drug development, EO771 is globally recognized as an indispensable, flagship in vitro and in vivo model for luminal B breast cancer. Because the cell line is natively derived from a C57BL/6 mouse, it can be orthotopically transplanted directly into the mammary fat pad of immunocompetent, syngeneic C57BL/6 host mice with a tumor take rate near 100%. This provides researchers with a fully functional immune microenvironment to evaluate the interplay between the tumor and the host immune system, spontaneous pulmonary metastasis, and the efficacy of modern immunotherapies (such as anti-PD-1 or anti-CTLA-4 antibodies).
Tissue	Mammary Gland
Cell Type	Epithelial cell
Strain	C57BL/6 (specifically C57BL/6J)
Disease	Mammary Gland Adenocarcinoma / Ductal Carcinoma
Morphology	Epithelial-like; elongated cells
Gender	Female
Growth Mode	Adherent
Biosafety Level	1 (Biosafety classification matches standard laboratory parameters for non-infectious murine cell lines)
Applications	1. Preferred syngeneic model for studying breast tumor immunology, dynamic changes in the tumor microenvironment (TME), and mechanisms of immune escape; 2. Preclinical testing of immunotherapies (including immune checkpoint inhibitors, cancer vaccines, and adoptive cell therapies); 3. Establishment of in vivo models for orthotopic breast cancer growth, local invasion, and spontaneous metastasis (particularly to the lungs, peritoneum, and brain); 4. Evaluation of responses to endocrine therapies and selective estrogen receptor modulators (SERMs); 5. Assessment of the biological roles of specific host genes in tumorigenesis and tumor progression using genetically modified C57BL/6 mouse strains (e.g., gene knockout models).
Shipped In	Dry ice
Storage Temperature	−196°C
Characteristics
Tumorigenic	Yes, highly tumorigenic in immunocompetent, syngeneic C57BL/6 mice.
Therapeutic Sensitivity	Natively sensitive to anti-estrogen treatments, selective estrogen receptor modulators (SERMs) such as tamoxifen, and modern immune checkpoint inhibitors targeting the PD-1/PD-L1 pathway.
Growth Kinetics	Robust adherent proliferation; population doubling time is approximately 24 to 36 hours.
Mycoplasma Test	Negative
Culture Conditions and Handling
Subculturing	1. Remove and discard all used complete culture medium from the culture vessel. 2. Gently rinse the cell monolayer with sterile, Ca2+/Mg2+-free PBS to neutralize residual serum. 3. Add 1.0 to 2.0 mL of pre-warmed 0.25% trypsin-0.53 mM EDTA solution (or Accutase) to completely cover the cells. 4. Incubate at 37°C for 2 to 5 minutes; monitor under an inverted microscope until the cells become rounded, loosen, and fully detach. Note: For cells that are difficult to detach, incubation at 37°C may be safely extended up to 8 minutes to facilitate complete dissociation. 5. Add an equal volume of complete culture medium to rapidly neutralize enzyme activity. 6. Centrifuge the cell suspension at approximately 300 × g for 3 to 5 minutes. Discard the supernatant, gently resuspend the cell pellet in fresh complete growth medium, and distribute the cells into new vessels.
Thawing Protocol Note	EO771 cells are sensitive to the alkalinity of the culture medium during the early stages after thawing. Before adding the thawed cell suspension, the culture vessel containing fresh complete growth medium should be placed in a 5% CO2 incubator for at least 15 minutes to allow the medium to reach and equilibrate within the normal physiological pH range (7.0 to 7.6).
Medium Renewal	2 to 3 times per week
Subcultivation Ratio	Split confluent cultures (70-80% density) at a standard ratio of 1:3 to 1:8.
Culture Conditions	Atmosphere: Air, 95%; CO2, 5%; Temperature: 37°C
Cryopreservation	90% Complete culture growth medium + 10% DMSO (or 50% DMEM + 40% FBS + 10% DMSO)

Cat.No.	Product Name	Price
CSC-RR00672	Luciferase Reporter Cell Line - EO771	Inquiry
CSC-RO01585	OVAL Stable Cell Line - EO771	Inquiry
CSC-RO01838	GFP/OVAL Stable Cell Line - EO771	Inquiry
CSC-RO02091	Luc/OVAL Stable Cell Line - EO771	Inquiry
CSC-RO02344	GFP/Luc/OVAL Stable Cell Line - EO771	Inquiry
CSC-RR01051	mScarlet/LUC Reporter Cell Line - EO771	Inquiry
CSC-RR01189	Nluc Reporter Cell Line - EO771	Inquiry

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* For research use only. Not intended for any clinical use.

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