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| General Information | |
| Organism | Homo sapiens, human |
| Cell Line Description | NCI-H1437 is a human non-small cell lung cancer (NSCLC) cell line derived from a stage I lung adenocarcinoma specimen obtained from a 60-year-old male patient. These cells exhibit an epithelial morphology, grow as an adherent monolayer under standard culture conditions, and retain key phenotypic characteristics of lung adenocarcinoma. Cytogenetically, the cell line displays an aneuploid karyotype characterized by various numerical and structural chromosomal alterations. At the molecular level, NCI-H1437 harbors mutations in key cancer-associated genes—including the TP53 tumor suppressor gene and MET—while expressing wild-type KRAS and EGFR. Due to its well-defined genomic profile and stable growth characteristics, NCI-H1437 has become a valuable in vitro model for studying lung adenocarcinoma biology, investigating MET kinase signaling pathways, evaluating novel targeted therapies and chemotherapeutic agents, and conducting functional genomic screens. |
| Tissue | Lung |
| Disease | Lung Adenocarcinoma; Non-Small Cell Lung Cancer (NSCLC) |
| Morphology | Epithelial |
| Gender | Male |
| Age | 60 years |
| Product Format | Frozen |
| Growth Mode | Adherent |
| Biosafety Level | 1 (Biosafety classification is based on U.S. Public Health Service Guidelines) |
| Applications | 1. Research on the pathogenesis of non-small cell lung cancer (NSCLC) and the MET signaling pathway; 2. Evaluation and screening of targeted therapies, small-molecule inhibitors, and chemotherapeutic agents; 3. Research on mechanisms underlying lung cancer cell proliferation, apoptosis, and drug resistance; 4. Functional genomic analysis, target validation, and CRISPR/Cas9 screening and validation. |
| Shipped In | Dry ice |
| Storage Temperature | −196°C |
| Characteristics | |
| Karyotype | Aneuploid |
| Tumorigenic | Yes, tumorigenic in immunocompromised (nude) mice |
| Genetic Profile | TP53 mutation; MET mutation; EGFR wild-type; KRAS wild-type |
| Mycoplasma Test | Negative |
| Culture Conditions and Handling | |
| Subculturing | 1. Remove and discard the culture medium. 2. Briefly rinse the cell monolayer with PBS (without Ca2+/Mg2+) to completely remove residual serum. 3. Add 1.0 to 2.0 mL of 0.25% trypsin-0.03% EDTA solution and incubate at 37°C until the cell monolayer detaches (typically 2 to 5 minutes). 4. Add fresh complete growth medium to neutralize the trypsin and gently pipette up and down to prepare a single-cell suspension. 5. Subculture the cell suspension into new culture vessels. |
| Medium Renewal | Every 2 to 3 days |
| Subcultivation Ratio | A split ratio of 1:3 to 1:6 is recommended |
| Culture Conditions | Atmosphere: Air, 95%; CO2, 5%; Temperature: 37°C |
| Cryopreservation | Complete growth medium supplemented with 5% to 10% (v/v) DMSO |
| Cat.No. | Product Name | Price |
|---|---|---|
| CSC-RO0154 | Cas9 Stable Cell Line - NCI-H1437 | Inquiry |
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