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| General Information | |
| Organism | Homo sapiens, human |
| Cell Line Description | Ramos is a human B-lymphoblastoid cell line established in 1975. It was isolated from the ascites of a 3-year-old Caucasian male diagnosed with American-type Burkitt lymphoma. Ramos cells grow exclusively in suspension, appearing as single round cells or loose multicellular clusters. In the fields of immunology, virology, and hematology, Ramos is recognized as the "gold standard" model for EBV-negative B cells. Because the cell line lacks an endogenous Epstein-Barr virus (EBV) genome yet is highly susceptible to EBV infection in vitro, it serves as an indispensable control system for dissecting the process of EBV-driven oncogenic transformation. Furthermore, given its expression of a functional B-cell receptor (BCR) and specific surface markers, Ramos is a cornerstone model for studying B-cell signal transduction, receptor activation, and antibody-mediated signaling networks. |
| Tissue | Blood / Lymphoid; derived from pleural/peritoneal metastatic site (Ascites) |
| Cell Type | B Lymphoblast |
| Population / Ancestry | Caucasian |
| Disease | Burkitt Lymphoma |
| Morphology | Lymphoblast-like; round cells |
| Gender | Male |
| Age | 3 years |
| Product Format | Frozen |
| Growth Mode | Suspension |
| Biosafety Level | 1 (Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.) |
| Applications | 1. An indispensable in vitro model for studying B-cell receptor (BCR) signaling, calcium mobilization, and tyrosine kinase activation cascades; 2. Standard target cells for investigating Epstein-Barr virus (EBV) infection dynamics, viral entry mechanisms, and the process of permanent transformation into EBV-positive cell lines; 3. Elucidating B-cell activation pathways mediated by interleukins (particularly IL-4 receptor-mediated signaling) and CD23/CD40; 4. Preclinical drug screening for B-cell malignancies and lymphomas, focusing on targeted monoclonal antibodies and apoptosis-inducing agents; 5. Establishing robust B-cell lymphoma xenograft models in immunodeficient mice. |
| Shipped In | Dry ice |
| Storage Temperature | −196°C |
| Characteristics | |
| Tumorigenic | Yes, highly tumorigenic; forms rapidly growing Burkitt-like B-cell tumors in highly immunocompromised host models. |
| Transformant | None; immortalized spontaneously from advanced, malignant pediatric clinical tumor tissue. |
| Oncogenic Driver | t(8;14) Chromosomal Translocation. Harbors the hallmark Burkitt lymphoma translocation which fuses the MYC proto-oncogene on chromosome 8 in-frame with the immunoglobulin heavy-chain (IGH) locus on chromosome 14, driving constitutive, high-level expression of MYC. |
| Viral Profile | EBV-negative (Epstein-Barr virus negative) but retains full susceptibility to in vitro infection and subsequent transformation. |
| Karyotype | Human hypodiploid/pseudodiploid karyotype. Typical modal chromosome number is 45 or 46. |
| Mycoplasma Test | Negative |
| Culture Conditions and Handling | |
| Subculturing | 1. Since these cells grow entirely in suspension (as single cells or loose aggregates), no trypsinization is required. 2. Gently shake or swirl the culture vessel to ensure the cells are evenly suspended. 3. Transfer the cell-containing medium to a sterile conical centrifuge tube. 4. Centrifuge at approximately 125 × g (or 1000 rpm) for 5 to 7 minutes. 5. Carefully aspirate or decant the supernatant, leaving the cell pellet undisturbed. 6. Gently resuspend the cell pellet in pre-warmed, fresh complete medium. |
| Thawing Protocol Note | Ramos cells are highly sensitive to the initial environment and local pH levels following thawing. To ensure successful recovery, culture flasks containing fresh complete medium should be pre-incubated in a 5% CO2 incubator for at least 15 minutes prior to seeding the thawed cell suspension; this ensures the medium's pH stabilizes within the physiological range of 7.0 to 7.6. |
| Medium Renewal | 2 to 3 times per week (or complete fluid change as needed depending on cell density and medium acidification) |
| Subcultivation Ratio | Split saturated suspension cultures at a ratio of 1:2 to 1:4. |
| Culture Conditions | Atmosphere: Air, 95%; CO2, 5%; Temperature: 37°C |
| Cryopreservation | 90% Complete culture medium + 10% DMSO (or 70% Basal medium + 20% FBS + 10% DMSO) |
| Cat.No. | Product Name | Price |
|---|---|---|
| CSC-RR0641 | Luciferase Reporter Cell Line - Ramos | Inquiry |
| CSC-RO01639 | OVAL Stable Cell Line - Ramos | Inquiry |
| CSC-RO01892 | GFP/OVAL Stable Cell Line - Ramos | Inquiry |
| CSC-RO02145 | Luc/OVAL Stable Cell Line - Ramos | Inquiry |
| CSC-RO02398 | GFP/Luc/OVAL Stable Cell Line - Ramos | Inquiry |
| CSC-RO02652 | Cas9 Stable Cell Line - Ramos | Inquiry |
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