HaCat Cell Line

Brief Introduction
Product List

General Information
Organism	Homo sapiens, human
Cell Line Description	The HaCaT cell line is an immortalized human keratinocyte cell line established in 1988. It originates from the long-term spontaneous transformation of adult skin keratinocytes under low-calcium, high-temperature conditions. HaCaT cells are non-tumorigenic and retain full epidermal differentiation capacity, mimicking the layering and keratinization processes of normal skin. Unlike many other immortalized cell lines, HaCaT cells retain a near-normal phenotype, making them a preferred in vitro model for skin biology, toxicology, and wound healing studies. They are widely used to study keratinization, skin inflammation, UV-induced DNA damage, and the efficacy of cosmetics and topical pharmaceuticals.
Tissue	Skin
Cell Type	Keratinocyte (Spontaneously Immortalized)
Morphology	Epithelial-like
Gender	Male
Age	62 years
Product Format	Frozen
Growth Mode	Adherent
Biosafety Level	1 (Biosafety classification is based on U.S. Public Health Service Guidelines)
Applications	1. Skin biology and epidermal differentiation studies 2. Skin toxicology and irritation testing 3. Wound healing and re-epithelialization studies 4. UV damage and photoprotection studies 5. Assessment of skin inflammatory signaling pathways (e.g., psoriasis or dermatitis models)
Shipped In	Dry ice
Storage Temperature	−196°C
Characteristics
Tumorigenic	No, does not form tumors in nude mice
Karyotype	Aneuploid; near-diploid with specific chromosomal alterations
Markers	Expresses typical basal and suprabasal keratins (K1, K10, K5, K14)
Differentiation	Capable of forming a stratified epidermis in organotypic culture
Mycoplasma Test	Negative
Culture Conditions and Handling
Subculturing	1. Remove and discard the culture medium. 2. Quickly wash the cell layer with Ca²⁺/Mg²⁺-free PBS buffer to remove all serum residue. 3. Add 2.0 to 3.0 mL of 0.25% trypsin-0.53 mM EDTA solution and observe under an inverted microscope until the cell layer disperses (usually 5 to 10 minutes). 4. Add complete culture medium to neutralize the trypsin and gently pipette to obtain a single-cell suspension. 5. Aliquot the cell suspension into new culture dishes.
Medium Renewal	2 to 3 times per week
Subcultivation Ratio	A split ratio of 1:3 to 1:8 is recommended
Culture Conditions	Atmosphere: Air, 95%; CO2, 5%; Temperature: 37°C
Cryopreservation	Complete growth medium supplemented with 5% to 10% (v/v) DMSO

Cat.No.	Product Name	Price
CSC-RR0335	GFP Reporter Cell Line - Hacat	Inquiry
CSC-RT2590	Human GJB2 Knockout Cell Line-HaCat	Inquiry
CSC-RR00767	Luciferase Reporter Cell Line - hacat	Inquiry
CSC-RO02530	Human TET2 Stable Cell Line - Hacat	Inquiry
CSC-RO02613	Cas9 Stable Cell Line - HaCaT	Inquiry

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* For research use only. Not intended for any clinical use.

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