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| General Information | |
| Organism | Homo sapiens, human |
| Cell Line Description | MG-63 is a human osteosarcoma cell line established by A. Billiau and colleagues in 1977. This cell line was derived from the primary bone tumor tissue of a 14-year-old male osteosarcoma patient. MG-63 cells exhibit a fibroblast-like or spindle-shaped morphology and grow in an adherent monolayer. Despite their malignant characteristics, these cells retain many key osteoblast phenotypic features, making them one of the most widely used in vitro models in bone biology research. MG-63 responds strongly to parathyroid hormone (PTH) and synthesizes high levels of interferon. It is used as a standard model for analyzing osteogenic processes, bone remodeling mechanisms, biocompatibility of biomaterials (e.g., orthopedic implant coatings), and testing novel bone-targeted therapies. |
| Tissue | Bone |
| Disease | Osteosarcoma |
| Morphology | Fibroblast-like; Spindle-shaped |
| Gender | Male |
| Age | 14 years |
| Product Format | Frozen |
| Growth Mode | Adherent |
| Biosafety Level | 1 (Biosafety classification is based on U.S. Public Health Service Guidelines) |
| Applications | 1. Research on osteoblast differentiation, matrix mineralization, and bone tissue engineering 2. Biocompatibility testing and cell adhesion analysis of orthopedic and dental biomaterials 3. Research on human interferon production, signaling cascades, and antiviral cell biology 4. Screening for novel chemotherapy regimens and targeted drugs for the treatment of childhood osteosarcoma 5. Construction of a human osteosarcoma xenograft model in immunodeficient mice |
| Shipped In | Dry ice |
| Storage Temperature | −196°C (Liquid nitrogen vapor phase) |
| Characteristics | |
| Tumorigenic | Yes, reliably forms osteolytic or fibroblastic tumors in immunocompromised mice |
| Karyotype | Aneuploid/heteroploid; the karyotype is extremely unstable, and the chromosome number is roughly in the region close to triploid or tetraploid (mode is about 63 to 69). |
| Genetic Profile | 1. TP53 status: rearranged/altered (p53 protein expression levels are unstable or abnormal) 2. RB1 status: retained, but signaling pathways are often dysregulated 3. Inducibility: highly sensitive to 1,25-dihydroxyvitamin D3 (1,25(OH)2D3), which upregulates alkaline phosphatase (ALP) activity and osteocalcin secretion. |
| Growth Kinetics | Rapid proliferation; doubling time typically spans 28 to 36 hours under standard maintenance conditions. |
| Mycoplasma Test | Negative |
| Culture Conditions and Handling | |
| Subculturing | 1. Remove and discard the used culture medium. 2. Gently wash the cell monolayer with Dulbecco's phosphate-buffered saline (DPBS) free of Ca²⁺/Mg²⁺ to neutralize any residual serum components. 3. Add 2.0 to 3.0 mL of 0.25% trypsin-0.53 mM EDTA solution to completely submerge the cell layer. 4. Incubate at 37°C for 2 to 6 minutes; observe under an inverted microscope until the cell layer has completely detached and is round. 5. Add an equal or greater volume of complete culture medium to the culture dish to thoroughly neutralize trypsin activity. 6. Aspirate the culture dish, centrifuge at 200–300 × g for 5 minutes, discard the supernatant, gently resuspend the cell clumps in fresh culture medium, and aliquot into new culture dishes. |
| Medium Renewal | 2 to 3 times per week |
| Subcultivation Ratio | A split ratio of 1:3 to 1:6 is standardly recommended for optimal maintenance. |
| Culture Conditions | Atmosphere: Air, 95%; CO2, 5%; Temperature: 37°C |
| Cryopreservation | 90% Complete growth medium + 10% DMSO (or 50% Basal medium + 40% FBS + 10% DMSO) |
| Cat.No. | Product Name | Price |
|---|---|---|
| CSC-RT0115 | CD276 Knockout Cell Line-MG63 | Inquiry |
| CSC-RO02642 | Cas9 Stable Cell Line - MG-63 | Inquiry |
| CSC-RR01212 | Luciferase Reporter Cell Line - MG63 | Inquiry |
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