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Gene Variant Library Service FAQs


Gene Variant Library Service FAQs

  1. What material do I have to supply for library synthesis?
  2. Can a library be subcloned in my vector?
  3. Are the genes sequencing verified?
  4. How are libraries supplied?
  5. What are some examples of what gene variant library can be used for?
  6. Do you offer library design services for randomizing specific residues of the DNA

1、What material do I have to supply for library synthesis?

No material at all. All we need for library creation is the sequence file, submitted electronically, and information about the position and nature of the sites you want to randomize. We can provide a quote for your project through our online ordering system. If needed, you can then relate detailed information about your library request to our production scientists prior to starting the project.

2、Can a library be subcloned in my vector?

Yes. For this purpose customers either send their own, individual vector or send us a commercially available vector.

3、Are the genes sequencing verified?

Creative Biogene conducts sequence verification during the gene libray synthesis process to ensure your synthesis product is as intended. For all gene variant library synthesis orders, we provide electronic data packages in parallel with your constructs. The data package includes the original sequencing data files from construct verification to facilitate your independent review.

4、How are libraries supplied?

Two standard options are available:

  1. As a set of linear DNA fragments, ready for restriction enzyme digestion and subcloning.
  2. Subcloned into any vector and delivered as a bacterial strain and plasmid preparation.

5、What are some examples of what gene variant library can be used for?

• Enhance or modulate protein stability
• Modify or combine enzyme properties
• Increase binding affinities of receptors, ligands and antibodies
• Optimize or alter signal peptide efficiencies
• Destroy protein function while retaining immunogenicity
• Combine and select natural polymorphisms
• Increase protein half life

6、Do you offer library design services for randomizing specific residues of the DNA?

Yes, we offer digitally designed gene libraries. We offer random mutation libraries as well as alanine scan library, degenerate codon library, triplet codon library, site saturation library, randomized mutant libraries etc.

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