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| General Information | |
| Organism | Mus musculus, mouse |
| Cell Line Description | MH-S is a continuously passaged murine alveolar macrophage cell line established by I. Mbawuike and H.B. Herscowitz in 1989. The cell line was derived from a cell population obtained via bronchoalveolar lavage (BAL) of a 7-week-old male mouse, followed by purification and selection based on plastic adherence. Researchers successfully immortalized the cell line using the Simian Virus 40 (SV40) oncogene. MH-S cultures exhibit a mixed phenotype, comprising both tightly adherent and suspension-growing cell populations. In the fields of immunology and respiratory research, MH-S is regarded as the international "gold standard" in vitro model for studying alveolar macrophage biology. These cells retain key functional characteristics of alveolar macrophages—including robust phagocytic activity, a lack of contact inhibition, esterase positivity, and the ability to specifically suppress splenic lymphocyte responses—making them an indispensable tool for investigating respiratory host defense mechanisms and pulmonary pathology. |
| Tissue | Lung |
| Cell Type | Alveolar Macrophage |
| Strain | BALB/c (specifically BALB/cJ) |
| Disease | Normal / Non-malignant tissue origin (Immortalized engineered line) |
| Morphology | Macrophage/Lymphoblast-like; irregular shape with visible pseudopodia |
| Gender | Male |
| Age | 7 weeks |
| Product Format | Frozen |
| Growth Mode | Mixed: Adherent and Suspension (cells expand simultaneously as a attached sheet and floating clusters) |
| Biosafety Level | 1 (Contains integrated SV40 DNA sequences but is verified negative for viral shedding) |
| Applications | 1. A "gold standard" model for simulating pulmonary immune responses, lung inflammation, and respiratory distress syndrome; 2. Investigation of host-pathogen interactions involving respiratory viruses, intracellular bacteria, and fungi; 3. In-depth analysis of the mechanisms underlying alveolar macrophage activation, phagocytosis, and stimulus-induced cytokine expression; 4. Evaluation of the toxicological characteristics and inflammatory effects of airborne particulate matter and nanomaterials; 5. Preclinical screening of novel anti-inflammatory drugs and immunomodulators targeting the lungs. |
| Shipped In | Dry ice |
| Storage Temperature | −196°C (Liquid nitrogen vapor phase only) |
| Characteristics | |
| Tumorigenic | No, non-tumorigenic under standard physiological baselines. |
| Transformant | Simian virus 40 (SV40) large T-antigen integration. |
| Secretory Capability | Constitutively synthesizes and secretes low levels of Interleukin-1 (IL-1). Secretion profiles are markedly amplified upon exposure to bacterial lipopolysaccharide (LPS). |
| Enzyme Cytochemistry | Demonstrates structural fidelity highly consistent with the primary tissue: positive for non-specific esterase (>98%) and completely negative for peroxidase activity. |
| Antigen Expression | Endogenously expresses the core macrophage marker CD11b (Mac-1), macrophage class II antigens (I-A/MHC class II), and the SV40 large T antigen. |
| Mycoplasma Test | Negative |
| Culture Conditions and Handling | |
| Subculturing | 1. Do not discard the suspended cells; the suspension contains a significant number of viable, active cells. 2. Collect all the spent medium containing the suspended cell population and transfer it to a sterile conical centrifuge tube. 3. Briefly rinse the remaining adherent cell layer with 0.25% Trypsin-0.53 mM EDTA solution to neutralize residual serum components; discard the rinse solution. 4. Add 1.0 to 2.0 mL of fresh Trypsin-EDTA solution and incubate at room temperature or 37°C until the adherent cells round up and detach. 5. Combine the detached cells with the previously collected medium containing suspended cells to stop the enzymatic reaction. 6. Centrifuge the mixture at approximately 125 × g for 5 to 7 minutes. Discard the supernatant, gently resuspend the cell pellet in fresh complete medium, and seed into a new culture vessel. |
| Medium Renewal | 2 to 3 times per week |
| Subcultivation Ratio | Split optimal, dense cultures at a standard ratio of 1:4 to 1:6. |
| Culture Conditions | Atmosphere: Air, 95%; CO2, 5%; Temperature: 37°C |
| Cryopreservation | 90% Complete culture medium + 10% DMSO (or 55% Complete medium + 40% FBS + 5% DMSO) |
| Cat.No. | Product Name | Price |
|---|---|---|
| CSC-RO02571 | Cas9 Stable Cell Line - MH-S | Inquiry |
| CSC-RR00881 | GFP Reporter Cell Line - MH-S | Inquiry |
| CSC-RR00930 | Luciferase Reporter Cell Line - MH-S | Inquiry |
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