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| General Information | |
| Organism | Mus musculus, mouse |
| Cell Line Description | P3U1 (P3X63Ag8U.1) is a murine myeloma/plasmacytoma cell line derived from a mineral oil-induced MOPC-21 tumor in a BALB/c mouse. Through subclone selection, a variant was obtained that is resistant to 8-azaguanine and deficient in hypoxanthine-guanine phosphoribosyltransferase (HGPRT). P3U1 is one of the most commonly used fusion partner cell lines for the generation of monoclonal antibody-producing hybridomas; because it neither synthesizes nor secretes immunoglobulin chains and cannot survive in HAT (hypoxanthine-aminopterin-thymidine) selection medium, it facilitates the efficient enrichment of hybridomas following cell fusion. The cell line grows in suspension and exhibits a lymphoblast-like morphology. P3U1 is utilized for the construction of stable transfectomas and is widely applied in research areas such as somatic cell hybridization, the engineering of antibody-secreting cells, and the genetics of B-cell myelomas. As a key reference for HGPRT-deficient selection systems and the characterization of hybridoma fusion partners, P3U1 is extensively employed to establish stable transfectomas that express recombinant antibodies or other target proteins. |
| Tissue | Plasmacytoma/myeloma (mineral-oil-induced) |
| Disease | Mouse Plasmacytoma (Myeloma) |
| Morphology | Lymphoblast-like |
| Gender | Female |
| Age | Adult (plasmacytoma-bearing BALB/c mouse) |
| Ethnicity | BALB/c inbred strain |
| Product Format | Frozen |
| Growth Mode | Suspension |
| Biosafety Level | 1 (Biosafety classification is based on U.S. Public Health Service Guidelines) |
| Applications | 1. Hybridoma fusion partner for monoclonal antibody production 2. HAT selection system: HGPRT-deficient partner that dies in HAT medium 3. B-cell lineage, plasmacytoma and myeloma biology research 4. Antibody engineering and recombinant antibody expression studies 5. Reference cell line for authentication of hybridoma panels 6. In vivo plasmacytoma/tumorigenicity studies in BALB/c mice |
| Shipped In | Dry ice |
| Storage Temperature | −196°C |
| Characteristics | |
| Tumorigenic | Yes; supports tumorigenicity studies in BALB/c mice |
| Karyotype | Aneuploid; parental P3X63Ag8 modal chromosome number approximately 60–65 |
| Genetic Profile | Hypoxanthine–guanine phosphoribosyltransferase (HGPRT) deficient; selected for resistance to 8-azaguanine; does not synthesize or secrete immunoglobulin chains (non-secretor) |
| Mycoplasma Test | Negative |
| Culture Conditions and Handling | |
| Subculturing | 1. Collect the cell suspension into a centrifuge tube and centrifuge at approximately 200 × g for 5 minutes. 2. Carefully discard the supernatant. 3. Resuspend the cell pellet in fresh complete growth medium and gently pipette to break up any cell aggregates. 4. Count the cells and adjust to the recommended seeding density with fresh medium. 5. Aliquot the cell suspension into new culture vessels. |
| Medium Renewal | 3 times per week |
| Subcultivation Ratio | Maintain at 3–9 × 10⁵ cells/mL; do not exceed 1 × 10⁶ cells/mL. Seed new vessels at approximately 4 × 10⁵ cells/mL. |
| Culture Conditions | Atmosphere: Air, 95%; CO2, 5%; Temperature: 37°C |
| Cryopreservation | Complete growth medium supplemented with 5% (v/v) DMSO |
| Cat.No. | Product Name | Price |
|---|---|---|
| CSC-RO0901 | Human TGFB1 Stable Cell Line - P3U1 | Inquiry |
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