KYSE-150 Cell Line

Brief Introduction
Product List

General Information
Organism	Homo sapiens, human
Cell Line Description	KYSE-150 is an esophageal squamous cell carcinoma (ESCC) cell line established in 1984 by Professor Shimada at Kyoto University, Japan. This cell line was derived from a primary tumor located in the upper esophagus (cervical segment) of a 49-year-old female patient; the tumor was classified as a poorly differentiated squamous cell carcinoma. The patient had undergone radiotherapy prior to tumor resection. KYSE-150 is one of the most widely utilized models within the KYSE series, distinguished by its robust proliferative capacity, highly aneuploid genome, and pronounced tumorigenicity. It exhibits an exceptionally high tumor formation rate in nude mice and is frequently employed in studies investigating the mechanisms of esophageal carcinogenesis, radiosensitivity, chemoresistance, and the screening of novel targeted therapies.
Tissue	Esophagus
Disease	Esophageal Squamous Cell Carcinoma (ESCC)
Morphology	Epithelioid; grows as an adherent monolayer
Gender	Female
Age	49 years
Product Format	Frozen
Growth Mode	Adherent
Biosafety Level	1 (Biosafety classification is based on U.S. Public Health Service Guidelines)
Applications	1. Research on the Pathogenesis, Invasion, and Metastasis of Esophageal Squamous Cell Carcinoma (ESCC) 2. Research on Tumor Radiosensitivity and DNA Damage Repair 3. Screening for Targeted Therapies Directed Against EGFR, Cyclin D1, or Other Dysregulated Pathways 4. Establishment of Allogeneic (Human-Derived) Tumor Xenograft Models 5. Investigation of the Impact of Epigenetic Alterations (e.g., p16 Gene Methylation) on the Cell Cycle
Shipped In	Dry ice
Storage Temperature	−196°C
Characteristics
Tumorigenic	Yes, highly tumorigenic in nude mice
Karyotype	Hypertriploid, characterized by complex chromosomal rearrangements and a high level of polyploidy (approximately 12% are polyploid).
Genetic Profile	1. Amplification: Amplification of the c-erb-B (approx. 8-fold) and Cyclin D1 (approx. 4-fold) genes. 2. Mutation: Presence of TP53 mutations. 3. Epigenetics: Silencing of the p16 (INK4a) gene due to CpG island methylation.
Growth Kinetics	The doubling time is approximately 13.7 to 25 hours (extremely rapid proliferation).
Mycoplasma Test	Negative
Culture Conditions and Handling
Subculturing	1. Discard the old culture medium. 2. Wash the cell monolayer with Ca²⁺/Mg²⁺-free PBS. 3. Add 0.25% Trypsin – 0.53 mM EDTA, and incubate at room temperature or 37°C for 2–5 minutes until the cells detach. 4. Add serum-containing complete medium to neutralize the enzymatic digestion, and gently pipette to create a single-cell suspension. 5. Seed the cells into new culture flasks at the appropriate ratio.
Medium Renewal	2 to 3 times per week
Subcultivation Ratio	The recommended ratio is 1:5 to 1:20 (due to its rapid growth rate, the ratio may be appropriately increased).
Culture Conditions	Atmosphere: Air, 95%; CO2, 5%; Temperature: 37°C
Cryopreservation	90% Complete growth medium + 10% DMSO

Cat.No.	Product Name	Price
CSC-RR0342	GFP Reporter Cell Line - KYSE-150	Inquiry
CSC-RT2649	Human MCM10 Knockout Cell Line-KYSE150	Inquiry
CSC-RO01194	Cas9 Stable Cell Line - KYSE-150	Inquiry
CSC-RR00686	Luciferase Reporter Cell Line - KYSE-150	Inquiry
CSC-RO01500	OVAL Stable Cell Line - KYSE-150	Inquiry
CSC-RO01753	GFP/OVAL Stable Cell Line - KYSE-150	Inquiry
CSC-RO02006	Luc/OVAL Stable Cell Line - KYSE-150	Inquiry
CSC-RO02259	GFP/Luc/OVAL Stable Cell Line - KYSE-150	Inquiry
CSC-RR01005	GFP/Luc Reporter Cell Line - KYSE-150	Inquiry

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