LA795 Cell Line

Brief Introduction
Product List

General Information
Organism	Mus musculus, mouse
Cell Line Description	LA-795 is a murine lung adenocarcinoma cell line, established in China in 1979. It originated from a spontaneous papillary lung tumor that developed in a male T739 inbred mouse. Following serial subcutaneous passage in syngeneic T739, 615, and KM mice, the tumor line exhibited a 100% rate of pulmonary metastasis; in 1982, an adherent cell line was isolated from this tumor via primary culture. The cells grow as an adherent monolayer and exhibit an epithelioid, rounded morphology. Their karyotype is hypotetraploid, with a modal chromosome number of approximately 66–69. LA-795 retains the metastatic phenotype of the parental tumor and is tumorigenic in immunocompetent syngeneic mice. Consequently, it is highly valuable for studying spontaneous hematogenous metastasis, tumor-stroma interactions, and for in vivo fluorescence imaging using variants labeled with eGFP (enhanced green fluorescent protein). This cell line serves as a critical syngeneic model for screening anti-angiogenic agents and immunomodulatory compounds, and it is widely used in research concerning lung cancer invasion, chromosomal instability, and the preclinical evaluation of anti-tumor drugs.
Tissue	Lung (spontaneous tumor)
Disease	Mouse Lung Adenocarcinoma
Morphology	Epithelial-like; round cells
Gender	Male
Age	Adult (spontaneous tumor in T739 inbred mouse)
Ethnicity	T739 inbred strain
Product Format	Frozen
Growth Mode	Adherent
Biosafety Level	1 (Biosafety classification is based on U.S. Public Health Service Guidelines)
Applications	1. Syngeneic subcutaneous and orthotopic lung-cancer models with 100% spontaneous lung metastasis in T739 mice 2. Investigation of tumor invasion, metastasis and tumor–stroma interaction 3. Cytogenetic and chromosomal-instability studies in murine lung cancer 4. eGFP-labeled LA-795 variants for in vivo fluorescence imaging of metastasis 5. Preclinical efficacy evaluation of antineoplastic agents in immunocompetent hosts 6. Tumor immunology studies in the syngeneic T739 background
Shipped In	Dry ice
Storage Temperature	−196°C
Characteristics
Tumorigenic	Yes; forms tumors in syngeneic T739, 615 and KM mice, with 100% spontaneous lung metastasis in T739
Karyotype	Hypotetraploid; modal chromosome number 66–69, with recurrent marker chromosomes 6q+ and 15q+ and non-random loss of chromosomes 4 and 14
Genetic Profile	p53 pathway alterations associated with the LA-795 tumor model
Mycoplasma Test	Negative
Culture Conditions and Handling
Subculturing	1. Remove and discard the culture medium. 2. Briefly rinse the cell monolayer with PBS (Ca²⁺/Mg²⁺-free) to thoroughly remove residual serum. 3. Add 2.0 to 3.0 mL of 0.25% Trypsin–0.53 mM EDTA solution, and observe under an inverted microscope until the cell monolayer has dispersed (typically takes 2 to 5 minutes). 4. Add 6.0 to 8.0 mL of complete growth medium, and gently pipette to prepare a single-cell suspension. 5. Aliquot the cell suspension into new culture vessels.
Medium Renewal	At subculturing, or 2 to 3 times per week
Subcultivation Ratio	A split ratio of 1:3 is recommended when the monolayer reaches approximately 80% confluence.
Culture Conditions	Atmosphere: Air, 95%; CO2, 5%; Temperature: 37°C
Cryopreservation	95% complete growth medium + 5% DMSO

Cat.No.	Product Name	Price
CSC-RR01204	Nluc Reporter Cell Line - LA795	Inquiry

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