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| General Information | |
| Organism | Homo sapiens, human |
| Cell Line Description | HSC-3 cell line was isolated from both the primary tumor and metastatic lymph node tissues of a 64-year-old Japanese male patient diagnosed with advanced, invasive squamous cell carcinoma of the tongue. HSC-3 cells exhibit an epithelial-like morphology and grow as an adherent monolayer. In the fields of oncology, oral pathology, and translational cancer biology, HSC-3 is globally recognized as a premier model for studying metastatic oral squamous cell carcinoma (OSCC). Compared to its counterpart HSC-2, HSC-3 demonstrates significantly higher invasive potential, robust secretion of matrix metalloproteinases (MMPs), and a propensity for rapid lymph node metastasis, making it a vital tool for investigating tumor cell invasion, invadopodia formation, and the epithelial-mesenchymal transition (EMT) process. |
| Tissue | Head and Neck; derived from Oral Cavity (Tongue) |
| Cell Type | Epithelial cell |
| Population / Ancestry | Japanese (East Asian) |
| Disease | Tongue Squamous Cell Carcinoma (OSCC) / Oral Cavity Cancer |
| Morphology | Epithelial-like; polygonal cells growing as an adherent monolayer |
| Gender | Male |
| Age | 64 years |
| Product Format | Frozen |
| Growth Mode | Adherent |
| Biosafety Level | 1 (Biosafety classification matches standard international parameters for non-infectious human tumor cells) |
| Applications | 1. A gold-standard in vitro model for studying the invasion, extracellular matrix remodeling, and mechanical properties of invadopodia in oral squamous cell carcinoma (OSCC); 2. In-depth analysis of matrix metalloproteinase (specifically MMP-2 and MMP-9) secretion, signaling pathways, and the dynamics of cell-matrix adhesion; 3. High-throughput preclinical screening of novel chemotherapeutic agents, EGFR inhibitors (such as cetuximab), and anti-metastatic targeted therapies; 4. Investigation of the molecular mechanisms underlying radioresistance, chemotherapy-induced cell death, and hypoxia-driven metastatic cascades; 5. Establishment of reliable orthotopic tongue cancer or subcutaneous xenograft models in immunodeficient mice to study regional lymph node metastasis. |
| Shipped In | Dry ice |
| Storage Temperature | −196°C |
| Characteristics | |
| Tumorigenic | Yes, highly tumorigenic and invasive; readily forms progressively growing squamous cell carcinomas with high metastatic potential when engrafted subcutaneously or orthotopically into the tongue of athymic nude mice. |
| Transformant | None; immortalized spontaneously from advanced primary and metastatic surgical tumor specimens. |
| Oncogenic Driver & Genomics | 1. TP53 status: Mutant; harbors a typical inactivating mutation in the TP53 gene. 2. EGFR status: Overexpression of functional epidermal growth factor receptor (EGFR). 3. Microsatellite status: Stable (MSS). |
| Karyotype | Human hyperdiploid/hypotriploid karyotype displaying complex structural rearrangements and numerical chromosomal aberrations typical of advanced OSCC. |
| Mycoplasma Test | Negative |
| Culture Conditions and Handling | |
| Subculturing | 1. Completely aspirate and discard the complete culture medium from the culture vessel. 2. Gently rinse the cell monolayer with sterile PBS (without Ca2+/Mg2+) to remove residual serum containing trypsin inhibitors. 3. Add 1.0 to 2.5 mL of 0.25% trypsin-0.53 mM (0.02%) EDTA solution, pre-warmed to the appropriate temperature, to cover the cell layer. 4. Incubate at 37°C for 2 to 5 minutes; monitor under an inverted microscope until the cells round up, detach, and fully slough off. 5. Add an equal volume of complete growth medium to immediately neutralize trypsin activity. 6. Gently pipette the suspension to disperse the cells into a single-cell suspension, then centrifuge at approximately 125 × g for 5 minutes; carefully aspirate the supernatant, gently resuspend the cell pellet in fresh complete medium, and distribute the cells into new culture vessels. |
| Thawing Protocol Note | HSC-3 cells are sensitive to pH changes during early post-thaw recovery. Prior to introducing the thawed vial contents, place the culture vessel containing fresh complete growth medium into the 5% CO2 incubator for at least 15 minutes to allow the medium to equilibrate to its normal physiological pH range (7.0 to 7.6). |
| Medium Renewal | 2 to 3 times per week |
| Subcultivation Ratio | Split confluent cultures (70-80% density) at a standard ratio of 1:3 to 1:8. |
| Culture Conditions | Atmosphere: Air, 95%; CO2, 5%; Temperature: 37°C |
| Cryopreservation | 90% Complete culture growth medium + 10% DMSO (or 50% Basal medium + 40% FBS + 10% DMSO) |
| Cat.No. | Product Name | Price |
|---|---|---|
| CSC-RO01182 | Cas9 Stable Cell Line - HSC3 | Inquiry |
| CSC-RR00679 | Luciferase Reporter Cell Line - HSC3 | Inquiry |
| CSC-RO01601 | OVAL Stable Cell Line - HSC3 | Inquiry |
| CSC-RO01854 | GFP/OVAL Stable Cell Line - HSC3 | Inquiry |
| CSC-RO02107 | Luc/OVAL Stable Cell Line - HSC3 | Inquiry |
| CSC-RO02360 | GFP/Luc/OVAL Stable Cell Line - HSC3 | Inquiry |
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