MOC2 Cell Line

Brief Introduction
Product List

General Information
Organism	Mus musculus, mouse
Cell Line Description	MOC2 is a highly aggressive murine oral squamous cell carcinoma (OSCC) cell line established by R. Uppaluri and colleagues. This cell line was generated through chemical carcinogenesis by inducing tumors in female C57BL/6 mice using 7,12-dimethylbenz[a]anthracene (DMBA). Within the widely utilized MOC preclinical model series—which also includes the relatively less aggressive MOC1—MOC2 exhibits a phenotypic profile characterized by poor differentiation, high invasiveness, and immunosuppression. MOC2 tumors are typified by low basal and inducible expression levels of MHC Class I molecules; this renders them weakly immunogenic and confers profound resistance to standard immune checkpoint blockade therapies, such as anti-PD-1 treatment. Given that MOC2 is fully syngeneic with the immunocompetent C57BL/6 mouse strain, it has emerged globally as the preferred model for investigating the tumor microenvironment of head and neck squamous cell carcinoma (HNSCC), mechanisms of immune evasion, tumor-induced orofacial pain, and novel combination immunotherapies.
Tissue	Oral cavity
Disease	Oral Squamous Cell Carcinoma (OSCC) / Head and Neck Cancer
Morphology	Epithelial-like
Gender	Female
Age	Adult
Product Format	Frozen
Growth Mode	Adherent
Biosafety Level	1 (Biosafety classification is based on U.S. Public Health Service Guidelines)
Applications	1. Establishment of a preclinical syngeneic model of aggressive, low-immunogenic head and neck cancer based on C57BL/6 mice. 2. Investigation of the molecular mechanisms driving immune evasion, regulatory T cell (Treg) infiltration, and reduced MHC expression. 3. Evaluation of strategies to overcome resistance to immune checkpoint blockade therapies (anti-PD-1/anti-CTLA-4). 4. Construction of models elucidating the mechanisms of tumor-driven angiogenesis, lymph node metastasis, and extracellular vesicle (EV) signaling. 5. Investigation of tumor-associated pain pathways and neuro-immune interaction mechanisms in orofacial tumors using an orthotopic tongue implantation model.
Shipped In	Dry ice / Liquid nitrogen vapor phase
Storage Temperature	−196°C
Characteristics
Tumorigenic	Yes, highly tumorigenic; spontaneously metastasizes to draining lymph nodes following flank or orthotopic transplantation
Induction Agent	Chemically transformed using 7,12-dimethylbenzanthracene (DMBA)
Immunogenic Status	"Cold" / weakly immunogenic; exhibits downregulation of both basal and inducible MHC Class I molecule expression, and recruits FOXP3+ regulatory T cells in vivo.
Genetic Profile	1. Kras mutation: Carries a solitary mutation at p.Gln61His. 2. Trp53 mutation: Carries a solitary nonsense mutation at p.Glu225Ter.
Growth Kinetics	Rapid in vitro expansion and rapid in vivo kinetics; outpaces MOC1 growth even at a 10-fold lower inoculation dose
Mycoplasma Test	Negative
Culture Conditions and Handling
Subculturing	1. Remove and discard the spent culture medium. 2. Gently rinse the cell monolayer with Ca²⁺/Mg²⁺-free Dulbecco's Phosphate-Buffered Saline (DPBS) to neutralize residual serum. 3. Add 2.0 to 3.0 mL of 0.25% Trypsin–0.53 mM EDTA solution, ensuring the cell layer is completely submerged. 4. Incubate at 37°C for 3 to 7 minutes; observe under an inverted microscope until the cell layer is visibly detached and the cells have rounded up. 5. Add an equal or greater volume of complete culture medium to thoroughly neutralize the trypsin activity. 6. Centrifuge the cell suspension at approximately 200-300 × g for 5 minutes; aspirate the supernatant, gently resuspend the cell pellet in fresh medium, and aliquot into new culture dishes.
Medium Renewal	2 to 3 times per week
Subcultivation Ratio	A split ratio of 1:4 to 1:8 is recommended for routine maintenance.
Culture Conditions	Atmosphere: Air, 95%; CO2, 5%; Temperature: 37°C
Cryopreservation	90% Complete growth medium + 10% DMSO (or 50% Basal medium + 40% FBS + 10% DMSO)

Cat.No.	Product Name	Price
CSC-RO02581	Cas9 Stable Cell Line - MOC2	Inquiry
CSC-RR00885	GFP Reporter Cell Line - MOC2	Inquiry
CSC-RR00934	Luciferase Reporter Cell Line - MOC2	Inquiry

The above is only part of a part of cell line products. If you don't find the cell line you want, Creative Biogene can also provide stable cell line generation service with the best prices and fastest turnaround time for you! Contact us for more information or to request a quote.

* For research use only. Not intended for any clinical use.

Quick Inquiry