SNU-C1 Cell Line

Brief Introduction
Product List

General Information
Organism	Homo sapiens, human
Cell Line Description	SNU-C1 is a human colorectal adenocarcinoma cell line established at Seoul National University in 1984. It was derived from the ascites of a 71-year-old Korean male patient, whose primary tumor was a moderately differentiated adenocarcinoma of the colon. It is one of the earlier members of the SNU series of colorectal cancer cell lines. In culture, these cells grow in suspension, forming round cell clusters. SNU-C1 harbors a homozygous TP53 p.Ser166Ter nonsense mutation and an in-frame APIP–SLC1A2 fusion; it is microsatellite stable (MSS). This cell line is also a widely cited model for studying chromothripsis, characterized by hundreds of clustered chromosomal rearrangements concentrated on chromosome 15. It is extensively used in research areas including gastrointestinal cancer biology, large-scale pharmacogenomics and CRISPR-based dependency screening, molecular subtyping of colorectal cancer, proteogenomic analysis, and cytogenetic studies of large-scale genomic rearrangements.
Tissue	Colon; derived from metastatic site (peritoneum, ascitic fluid)
Disease	Colon Adenocarcinoma (Colorectal Cancer); moderately differentiated
Morphology	Round cells growing as floating aggregates and clusters
Gender	Male
Age	71 years
Ethnicity	Korean (Asian)
Product Format	Frozen
Growth Mode	Suspension
Biosafety Level	1 (Biosafety classification is based on U.S. Public Health Service Guidelines)
Applications	1. Biological research on gastrointestinal cancers based on the SNU colorectal cancer cell line atlas; 2. Large-scale pharmacogenomics and drug response profiling; 3. Genome-wide loss-of-function dependency screening using CRISPR and shRNA; 4. Cytogenetic research on chromothripsis and large-scale chromosomal rearrangements; 5. Molecular subtyping and proteogenomic research in colorectal cancer.
Shipped In	Dry ice
Storage Temperature	−196°C
Characteristics
Doubling Time	Approximately 31 hours
Karyotype	Near-triploid (ploidy approximately 3.2–3.4); modal chromosome number not reported. Canonical chromothripsis model with clustered rearrangements concentrated on chromosome 15
Microsatellite Instability	Stable (MSS)
Genetic Profile	TP53 p.Ser166Ter (c.497C>A), homozygous; APIP–SLC1A2 in-frame gene fusion
Expression Markers	Epithelial origin confirmed by quantitative proteome profiling
Mycoplasma Test	Negative
Culture Conditions and Handling
Subculturing	1. Collect the cell suspension into a centrifuge tube and centrifuge at approximately 200 × g for 5 minutes. 2. Carefully discard the supernatant. 3. Resuspend the cell pellet in fresh complete growth medium and gently pipette to break up any cell aggregates. 4. Count the cells and adjust to the recommended seeding density with fresh medium. 5. Aliquot the cell suspension into new culture vessels.
Medium Renewal	Every 3 to 5 days
Subcultivation Ratio	Dilute the cell suspension with fresh complete growth medium at a ratio of 1:2 to 1:4; refer to the batch-specific information for the recommended dilution.
Culture Conditions	Atmosphere: Air, 95%; CO2, 5%; Temperature: 37°C
Cryopreservation	Complete growth medium supplemented with 5% (v/v) DMSO

Cat.No.	Product Name	Price
CSC-RO0175	Cas9 Stable Cell Line - SNU-C1	Inquiry

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* For research use only. Not intended for any clinical use.

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