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| General Information | |
| Organism | Homo sapiens, human |
| Cell Line Description | ECA-109 is a human esophageal squamous cell carcinoma cell line with adherent epithelial-like growth. It has been used extensively to investigate tumor-cell proliferation, apoptosis, invasion, three-dimensional growth, xenograft formation, molecular signaling, and acquired drug resistance in esophageal cancer. Because some distributed stocks have been reported to contain HeLa contamination, research use should be restricted to material supported by a lot-specific STR profile and current contamination testing. |
| Cell Type | Human esophageal squamous carcinoma cell |
| Tissue of Origin | Esophagus |
| Disease | Esophageal squamous cell carcinoma |
| Sex | Female |
| Age at Sampling | Not reported |
| Population | Chinese |
| Morphology | Epithelial-like |
| Product Format | Frozen |
| Growth Mode | Adherent |
| Applications | 1. Esophageal squamous cell carcinoma biology and disease-mechanism research 2. Cell proliferation, apoptosis, migration, adhesion, and invasion assays 3. Three-dimensional spheroid and organ-culture invasion studies 4. Xenograft growth, angiogenesis, and therapeutic-response research 5. Anticancer drug screening and mechanism-of-action studies 6. Cisplatin- and paclitaxel-resistance research using separately derived resistant sublines 7. Molecular signaling, gene-regulation, and functional-genomics workflows 8. Comparative studies with independently authenticated esophageal cancer models |
| Storage Conditions | Store below −150°C, preferably in the vapor phase of liquid nitrogen, for long-term preservation |
| Characteristics | |
| STR Profile | A human-specific STR profile is available. Reported alleles include AMEL: X; CSF1PO: 10; D13S317: 11; D16S539: 12; D18S51: 12,16; D21S11: 30; D3S1358: 17; D5S818: 12; D7S820: 8,12; D8S1179: 13,14; FGA: 25; Penta D: 9; Penta E: 16; TH01: 9; TPOX: 8; and vWA: 16,17. |
| Invasive Phenotype | The line has been used to study invasive behavior in three-dimensional spheroidal aggregate and organ-culture systems, supporting applications in cell-adhesion, migration, and invasion research. |
| Xenograft Capability | ECA-109 cells have been used to generate tumors in immunodeficient mice, supporting in vivo studies of tumor growth, proliferation, invasiveness, angiogenesis, and treatment response. |
| Drug-Resistance Derivatives | Cisplatin-resistant and paclitaxel-resistant derivatives have been established from ECA-109. These are distinct selected sublines; their resistance phenotypes and doubling times should not be assigned to the parental culture. |
| Mycoplasma Test | Negative |
| Culture Conditions and Handling | |
| Culture Conditions | 37°C in a humidified atmosphere containing 5% CO₂ |
| Passage Endpoint | Subculture at approximately 80–90% confluence; avoid prolonged over-confluence |
| Subculturing | 1. Pre-warm complete medium and balanced salt solution to 37°C. 2. Remove the spent medium and rinse the adherent monolayer gently once or twice with calcium- and magnesium-free balanced salt solution. 3. Add sufficient 0.05% trypsin–EDTA to cover the cell layer and incubate at room temperature or 37°C. 4. Examine the culture every 30–60 seconds and terminate dissociation when cells contract, round up, and can be gently dislodged; the reported digestion time is generally 1–5 minutes. 5. Add complete medium to stop enzyme activity and gently pipette to obtain an even cell suspension while minimizing bubbles. 6. Transfer the required aliquot to fresh culture vessels and return to 37°C and 5% CO₂. |
| Subcultivation Ratio | A split ratio of approximately 1:2 to 1:5 is supported by the reported routine passage procedure; adjust according to growth rate and experimental needs |
| Thawing and Recovery | Thaw the vial rapidly in a 37°C water bath while keeping the cap above the waterline; do not vortex. Disinfect the exterior before opening. Centrifuge the thawed suspension at approximately 500 × g for 2–5 minutes, remove the supernatant without disturbing the pellet, gently resuspend in fresh complete medium, and seed into a suitable vessel. Replace the medium on the following day according to attachment and recovery. |
| Cryopreservation | A reported freezing formulation consists of high-glucose DMEM with 20% fetal bovine serum and 10% DMSO. Freeze at a controlled rate before transfer to liquid nitrogen. |
| Cat.No. | Product Name | Price |
|---|---|---|
| CSC-RR0618 | Luciferase Reporter Cell Line - ECA-109 | Inquiry |
| CSC-RO01586 | OVAL Stable Cell Line - ECA-109 | Inquiry |
| CSC-RO01839 | GFP/OVAL Stable Cell Line - ECA-109 | Inquiry |
| CSC-RO02092 | Luc/OVAL Stable Cell Line - ECA-109 | Inquiry |
| CSC-RO02345 | GFP/Luc/OVAL Stable Cell Line - ECA-109 | Inquiry |
| CSC-RR01190 | GFP/Luc Reporter Cell Line - ECA109 | Inquiry |
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