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| General Information | |
| Organism | Homo sapiens, human |
| Cell Line Description | SNU-449 is a human hepatocellular carcinoma cell line derived from a primary liver cancer specimen obtained from a 52-year-old Asian male patient. The cell line exhibits an epithelial morphology, grows as an adherent monolayer in culture, and retains the core phenotypic characteristics of human hepatocellular carcinoma. Cytogenetically, the cell line displays an aneuploid karyotype characterized by complex numerical and structural chromosomal abnormalities. At the molecular level, SNU-449 harbors somatic mutations in key liver cancer-associated genes—including the tumor suppressor gene TP53—but lacks hepatitis virus integration. Due to its well-defined malignant biological background and favorable growth characteristics, SNU-449 has become a widely used in vitro model for investigating the mechanisms of hepatocarcinogenesis and signaling pathways, evaluating novel small-molecule drugs, exploring mechanisms of chemotherapy resistance, and conducting high-throughput functional genomic screens in oncology research. |
| Tissue | Liver |
| Disease | Hepatocellular Carcinoma; Liver Cancer |
| Morphology | Epithelial |
| Gender | Male |
| Age | 52 years |
| Product Format | Frozen |
| Growth Mode | Adherent |
| Biosafety Level | 1 (Biosafety classification is based on U.S. Public Health Service Guidelines) |
| Applications | 1. Research on the biology of hepatocellular carcinoma (HCC) and the molecular pathways underlying hepatocarcinogenesis; 2. Screening and evaluation of candidate chemotherapeutic and targeted therapeutic agents; 3. Investigation of mechanisms underlying chemotherapy resistance and tumor invasion in liver cancer; 4. Functional genomic analysis, target identification, and validation via CRISPR/Cas9 screening. |
| Shipped In | Dry ice |
| Storage Temperature | −196°C |
| Characteristics | |
| Karyotype | Aneuploid |
| Tumorigenic | Yes, tumorigenic in immunocompromised mice |
| Genetic Profile | TP53 mutation; CTNNB1 wild-type |
| Mycoplasma Test | Negative |
| Culture Conditions and Handling | |
| Subculturing | 1. Remove and discard the culture medium. 2. Briefly rinse the cell monolayer with PBS (without Ca2+/Mg2+) to completely remove residual serum. 3. Add 1.0 to 2.0 mL of 0.25% trypsin-0.03% EDTA solution and incubate at 37°C until the cell monolayer detaches (typically 3 to 6 minutes). 4. Add fresh complete growth medium to neutralize the trypsin and gently pipette up and down to create a single-cell suspension. 5. Subculture the cell suspension into new culture vessels. |
| Medium Renewal | Every 2 to 3 days |
| Subcultivation Ratio | A split ratio of 1:3 to 1:6 is recommended |
| Culture Conditions | Atmosphere: Air, 95%; CO2, 5%; Temperature: 37°C |
| Cryopreservation | Complete growth medium supplemented with 5% to 10% (v/v) DMSO |
| Cat.No. | Product Name | Price |
|---|---|---|
| CSC-RO0162 | Cas9 Stable Cell Line - SNU-449 | Inquiry |
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