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| General Information | |
| Organism | Homo sapiens, human |
| Cell Line Description | U937 was established by Sundström and Nilsson in 1974 from the pleural effusion of a 37-year-old male patient suffering from diffuse histiocytic lymphoma. It is one of the few human-derived monocytic cell lines available. U937 cells exhibit characteristics of monocytes and can be induced to differentiate into macrophage-like cells with phagocytic capabilities through the use of various inducing agents (such as PMA, Vitamin D3, and IFN-γ). As a classic immunological model, it is widely utilized to investigate monocyte-macrophage differentiation, inflammatory responses, cytokine production, chemotaxis, phagocytosis, and the cytotoxicity of anti-tumor drugs. |
| Tissue | Pleural effusion |
| Disease | Histiocytic Lymphoma |
| Morphology | Monocyte-like; Round, individual cells |
| Gender | Male |
| Age | 37 years |
| Product Format | Frozen |
| Growth Mode | Suspension |
| Biosafety Level | 1 (Biosafety classification is based on U.S. Public Health Service Guidelines) |
| Applications | 1. Investigation of the molecular mechanisms underlying monocyte-to-macrophage differentiation 2. Studies on the expression and regulation of inflammatory mediators (e.g., cytokines, chemokines) 3. Screening of anti-inflammatory drugs and immunomodulators 4. Analysis of apoptosis pathways and assessment of anti-tumor drug sensitivity 5. Studies on viral infections (e.g., replication of HIV or Dengue virus in monocytes) |
| Shipped In | Dry ice |
| Storage Temperature | −196°C |
| Characteristics | |
| Tumorigenic | Yes, in immunocompromised mice |
| Karyotype | Aneuploid; modal number = 55–58; complex chromosomal rearrangements are present. |
| Surface Markers | Expression of CD11b, CD13, CD14 (enhanced after induction), CD15, CD33 |
| Growth Kinetics | The doubling time is approximately 24–30 hours. |
| Mycoplasma Test | Negative |
| Culture Conditions and Handling | |
| Subculturing | 1. Suspension cells do not require enzymatic digestion. 2. Collect the cells by centrifugation (e.g., 1000 rpm for 5 minutes). 3. Discard the supernatant and resuspend the cells in fresh culture medium. 4. Inoculate into a new culture flask. 5. It is recommended to maintain the cell density between 2 x 10^5 and 1 x 10^6 cells/mL. |
| Medium Renewal | Every 2 to 3 days |
| Subcultivation Ratio | 1:3 to 1:5 |
| Culture Conditions | Atmosphere: Air, 95%; CO2, 5%; Temperature: 37°C |
| Cryopreservation | 90% Complete growth medium + 10% DMSO |
| Cat.No. | Product Name | Price |
|---|---|---|
| CSC-RR0114 | GFP Stable Cell Line-U937 | Inquiry |
| RTA-0009 | U937-Type I IFN Assay Cells | Inquiry |
| RTA-0017 | U937-GM-CSF Assay Cells | Inquiry |
| CSC-RR0545 | Luciferase Reporter Cell Line - U937 | Inquiry |
| CSC-RO01577 | OVAL Stable Cell Line - U937 | Inquiry |
| CSC-RO01830 | GFP/OVAL Stable Cell Line - U937 | Inquiry |
| CSC-RO02083 | Luc/OVAL Stable Cell Line - U937 | Inquiry |
| CSC-RO02336 | GFP/Luc/OVAL Stable Cell Line - U937 | Inquiry |
| CSC-RO02651 | Cas9 Stable Cell Line - U-937 | Inquiry |
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