NCI-H146 Cell Line

Brief Introduction
Product List

General Information
Organism	Homo sapiens, human
Cell Line Description	NCI-H146 is a human small-cell lung cancer (SCLC) cell line established in 1979 by A.F. Gazdar and J.D. Minna at the NCI–Navy Medical Oncology Branch. The cell line was derived from a bone marrow metastasis in a 59-year-old white male patient with SCLC. These cells grow in suspension culture as floating multicellular aggregates and are tumorigenic in nude mice. NCI-H146 retains the neuroendocrine features characteristic of SCLC, including high expression of neuron-specific enolase, brain-type creatine kinase, and L-dopa decarboxylase, as well as bombesin-like immunoreactivity; the cells also test positive for keratin and vimentin. Genetically, the cell line harbors a homozygous truncating mutation in RB1, a heterozygous frameshift mutation in TP53, and two truncating mutations in KMT2D; it is classified as microsatellite stable (MSS). NCI-H146 is widely used in SCLC research, including studies on neuropeptide autocrine signaling, BCL-2 family inhibitors, PARP1-targeted therapies, responses to etoposide-platinum combination chemotherapy, and DNA demethylation strategies.
Tissue	Bone marrow (metastatic site)
Disease	Small Cell Lung Cancer (SCLC)
Morphology	Epithelial-like; floating multicellular aggregates
Gender	Male
Age	59 years
Ethnicity	Caucasian
Product Format	Frozen
Growth Mode	Suspension (multicellular aggregates)
Biosafety Level	1 (Biosafety classification is based on U.S. Public Health Service Guidelines)
Applications	1. Small cell lung cancer biology and APUD (amine precursor uptake and decarboxylation) features 2. Neuropeptide autocrine and paracrine signaling, including bombesin/GRP axis research 3. Etoposide, platinum and PARP inhibitor sensitivity and chemoresistance studies 4. Loss of RB1 and TP53 function in SCLC and synthetic-lethal screens 5. Epigenetic and Jumonji demethylase-targeted therapy research in SCLC 6. Pharmacogenomic and proteomic profiling
Shipped In	Dry ice
Storage Temperature	−196°C
Characteristics
Tumorigenic	Yes, forms tumors in nude mice
Karyotype	Near-triploid; modal chromosome number 68 (range 66–71) with at least 15 common marker chromosomes
Microsatellite Instability	Stable (MSS)
Expression Markers	Positive for neuron-specific enolase, brain-type creatine kinase, L-DOPA decarboxylase, bombesin-like immunoreactivity, keratin, vimentin and IGF-II. Negative for neurofilament triplet protein, vasopressin, oxytocin and gastrin-releasing peptide
Mycoplasma Test	Negative
Culture Conditions and Handling
Subculturing	1. Collect the cell suspension into a centrifuge tube and centrifuge at approximately 200 × g for 5 minutes. 2. Carefully discard the supernatant. 3. Resuspend the cell pellet in fresh complete growth medium and gently pipette to break up any cell aggregates. 4. Count the cells and adjust to the recommended seeding density with fresh medium. 5. Aliquot the cell suspension into new culture vessels.
Medium Renewal	2 to 3 times per week
Subcultivation Ratio	Maintain at 1-2 × 10⁵ cells/mL; split at a ratio of 1:2 to 1:6 when aggregates become dense.
Culture Conditions	Atmosphere: Air, 95%; CO2, 5%; Temperature: 37°C
Cryopreservation	Complete growth medium supplemented with 5% (v/v) DMSO

Cat.No.	Product Name	Price
CSC-RO0899	Human CD274 Stable Cell Line - NCI-H146	Inquiry

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