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| General Information | |
| Organism | Rattus norvegicus, rat |
| Cell Line Description | RIN-m5F is a conditionally stable mouse pancreatic β-cell clone derived from X-ray irradiation-induced transformation of pancreatic islet tissue. Isolated by A.F. Gazdar, H. Oie, and colleagues, it is a specific clonal derivative of the parental RIN-m mouse islet cell line. This cell line originates from orthotopic pancreatic tumors in adult male New England Deaconess Hospital (NEDH) rats. RIN-m5F is a globally recognized model system for diabetes research and a primary tool for evaluating pancreatic β-cell biology, glucose-stimulated pathways, and insulin biokinetics. Unlike the parental cell line, RIN-m5F cells primarily produce and secrete insulin, while exhibiting high basal levels of L-DOPA decarboxylase (a classic marker of amine precursor uptake and decarboxylation/APUD activity), but do not produce somatostatin. |
| Tissue | Pancreas; Islets of Langerhans |
| Cell Type | Pancreatic Beta Cell |
| Disease | Rat Insulinoma |
| Morphology | Epithelial-like |
| Gender | Male |
| Age | 666 days (Adult) |
| Product Format | Frozen |
| Growth Mode | Adherent |
| Biosafety Level | 1 (Biosafety classification is based on U.S. Public Health Service Guidelines) |
| Applications | 1. In vitro screening and construction of pathogenesis models for type 1 and type 2 diabetes. 2. Elucidation of cellular mechanisms regulating insulin synthesis, packaging, and secretion kinetics. 3. Testing for potential protective compounds against streptozotocin (STZ)-induced oxidative stress and cytotoxicity. 4. Analysis of gene expression changes in β-cell transcription factors (including PDX-1, Glut-2, and GCK). 5. Investigation of cytokine-mediated β-cell damage pathways and inflammatory cascades. |
| Shipped In | Dry ice |
| Storage Temperature | −196°C (Liquid nitrogen vapor phase; storage at −70°C results in rapid viability loss) |
| Characteristics | |
| Tumorigenic | Yes, retains tumorigenic capacity in syngeneic or immunocompromised host systems |
| Secretory Profile | Insulin-positive; L-dopa decarboxylase-positive; Somatostatin-negative |
| Transformant | Malignantly transformed via experimental X-Ray irradiation of islet architecture |
| Growth Kinetics | Moderate to rapid adherent monolayer expansion; exhibits heightened sensitivity to excessive alkalinity during initial handling phases |
| Mycoplasma Test | Negative |
| Culture Conditions and Handling | |
| Subculturing | 1. Completely remove and discard the used culture medium. 2. Gently wash the cell monolayer with Dulbecco's phosphate-buffered saline (DPBS) free of Ca²⁺/Mg²⁺ to remove residual serum components. 3. Add 1.0 to 2.0 mL of 0.25% trypsin-0.53 mM EDTA solution to completely submerge the cell layer. 4. Incubate at 37°C for 1 to 2 minutes; observe carefully under an inverted microscope. These cells may adhere loosely; stop incubation immediately once 90% of the cells become rounded and begin to detach. 5. Immediately add 4.0 to 6.0 mL of preheated complete culture medium to completely neutralize trypsin activity. 6. Centrifuge at approximately 200 × g (or 1000 rpm) for 4 to 5 minutes, decant the liquid, gently resuspend in fresh culture medium, and aliquot into new containers. |
| Thawing Protocol Note | During the recovery process after thawing, it is crucial to avoid excessively alkaline culture medium. Before adding thawed cells, the culture vessel containing complete culture medium should be placed in an incubator for at least 15 minutes to allow the pH of the medium to balance to the normal physiological range (7.0 to 7.6). |
| Medium Renewal | 2 to 3 times per week |
| Subcultivation Ratio | A split ratio of 1:2 to 1:4 is standardly recommended for stable maintenance. |
| Culture Conditions | Atmosphere: Air, 95%; CO2, 5%; Temperature: 37°C |
| Cryopreservation | 90% Complete growth medium + 10% DMSO (or 50% Basal medium + 40% FBS + 10% DMSO) |
| Cat.No. | Product Name | Price |
|---|---|---|
| CSC-RO02595 | Cas9 Stable Cell Line - RIN-M5F | Inquiry |
| CSC-RR00917 | GFP Reporter Cell Line - RIN-m5F | Inquiry |
| CSC-RR00969 | Luciferase Reporter Cell Line - RIN-m5F | Inquiry |
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