NIT-1 Cell Line

Brief Introduction
Product List

General Information
Organism	Mus musculus, mouse
Cell Line Description	NIT-1 is an immortalized pancreatic β-cell line established in 1990 by Edward H. Leiter and colleagues at The Jackson Laboratory. This cell line was derived from a transgenic non-obese diabetic (NOD) mouse carrying the SV40 large T antigen under the control of the rat insulin promoter (RIP). NIT-1 cells grow as an adherent monolayer and exhibit an epithelioid morphology, frequently forming loose aggregates or clusters. Globally, NIT-1 is recognized as an indispensable model for Type 1 diabetes (T1D) research in the fields of endocrinology, metabolism, and autoimmunity. Derived from the NOD mouse strain—a primary animal model for autoimmune diabetes—NIT-1 cells naturally express β-cell autoantigens (such as insulin and GAD65), making them a vital tool for studying autoimmune-mediated β-cell destruction, cytokine toxicity, and the mechanisms of insulin secretion.
Tissue	Pancreas; derived from pancreatic islet cells (Beta cells)
Cell Type	Pancreatic Beta Cell
Strain	NOD/Lt (Non-Obese Diabetic)
Disease	Normal / Transgenic Autoimmune Beta-Cell Model (RIP-Tag1)
Morphology	Epithelial-like; grows as adherent clusters
Gender	Female
Age	Adult transgenic mouse (10 to 12 weeks old)
Product Format	Frozen
Growth Mode	Adherent (tends to form multi-layered aggregates)
Biosafety Level	1 or 2 (Depends on regional institutional guidelines regarding integrated SV40 T-antigen viral sequences)
Applications	1. Directly simulate the pathogenesis of autoimmune-mediated β-cell destruction, insulitis, and type 1 diabetes in vitro; 2. Evaluate the cytotoxic effects of cytotoxic T lymphocytes (CTLs) and the key targets of apoptosis mediated by pro-inflammatory cytokines (such as TNF-α, IL-1β, and IFN-γ); 3. Elucidate the pathways of insulin biosynthesis, processing, and glucose-stimulated insulin secretion (GSIS); 4. Screen for novel anti-diabetic compounds, β-cell protective agents, and immunomodulatory drugs; 5. Establish syngeneic or allogeneic β-cell transplantation models using NOD mouse strains.
Shipped In	Dry ice
Storage Temperature	−196°C
Characteristics
Tumorigenic	Yes; forms insulinomas when engrafted into syngeneic or immunocompromised host mice.
Transformant	Simian virus 40 (SV40) large T-antigen driven by the rat insulin promoter (RIP-Tag1).
Secretory Profile	Natively produces and secretes intact insulin and proinsulin. The cells respond to glucose and secretagogues (e.g., glibenclamide, IBMX) with elevated insulin release, though glucose responsiveness may attenuate over extended passaging.
Ultrastructure	Electron microscopy confirms the presence of classic beta-cell secretory granules with dense crystalline cores surrounded by a clear halo.
Growth Kinetics	Slow to moderate adherent expansion; population doubling time spans approximately 36 to 48 hours.
Mycoplasma Test	Negative
Culture Conditions and Handling
Subculturing	1. Remove and discard the spent complete culture medium from the culture vessel. 2. Gently rinse the cell monolayer with sterile PBS (without Ca2+ and Mg2+) to remove residual serum containing trypsin inhibitors. 3. Add 1.0 to 2.5 mL of 0.25% trypsin-0.53 mM (0.02%) EDTA solution, pre-warmed to the appropriate temperature, to cover the cell layer. 4. Incubate at 37°C for 3 to 7 minutes, monitoring closely under an inverted microscope. Gently tapping the culture flask helps detach cell clumps. 5. Add an equal volume of pre-warmed complete growth medium to immediately neutralize trypsin activity. 6. Gently pipette the cell suspension up and down to dissociate cell clumps into a single-cell suspension; then centrifuge at approximately 125 × g for 5 minutes, aspirate the supernatant, resuspend the cells in fresh growth medium, and distribute them into new culture vessels.
Thawing Protocol Note	NIT-1 cells are sensitive to pH changes during early post-thaw recovery. Prior to introducing the thawed vial contents, place the culture vessel containing fresh complete growth medium into the 5% CO2 incubator for at least 15 minutes to allow the medium to equilibrate to its normal physiological pH range (7.0 to 7.6).
Medium Renewal	Every 2 to 3 days
Subcultivation Ratio	Passage cultures at 70%–80% confluence at a standard split ratio of 1:2 to 1:4. Maintain a seeding density of at least 3.0 X 10^4 to 5.0 X 10^4 cells/cm2.
Culture Conditions	Atmosphere: Air, 95%; CO2, 5%; Temperature: 37°C
Cryopreservation	90% Complete culture growth medium + 10% DMSO

Cat.No.	Product Name	Price
CSC-RR00700	Luciferase Reporter Cell Line - NIT-1	Inquiry
CSC-RO01315	Mouse Mettl3 Stable Cell Line - NIT-1	Inquiry

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* For research use only. Not intended for any clinical use.

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