Our promise to you:
Guaranteed product quality, expert customer support.
24x7 CUSTOMER SERVICE
CONTACT US TO ORDER
| General Information | |
| Organism | Homo sapiens, human |
| Cell Line Description | BPH-1 is an immortalized human prostatic epithelial cell line established by S.W. Hayward and his colleagues in 1995. This cell line was derived from primary prostatic tissue obtained from a 68-year-old male patient diagnosed with benign prostatic hyperplasia (BPH). Its immortalization was successfully achieved through the transduction of the Simian Virus 40 (SV40) large T antigen, supplemented by a neomycin resistance cassette. BPH-1 is globally recognized as the premier non-cancerous surrogate model for studying prostatic growth and physiology. The cell line naturally exhibits a cytokeratin fingerprint characteristic of prostatic luminal epithelial cells. It is widely utilized to investigate cellular differentiation, interactions with stromal components during tissue remodeling, the molecular mechanisms driving BPH progression, and the metabolic transformations occurring during the early stages of prostatic carcinogenesis. |
| Tissue | Prostate |
| Disease | Benign Prostatic Hyperplasia (BPH) |
| Morphology | Epitheloid / Epithelial-like; grows as an adherent monolayer |
| Gender | Male |
| Age | 68 years |
| Product Format | Frozen |
| Growth Mode | Adherent Monolayer |
| Biosafety Level | 1 (Contains integrated, non-infectious fragments of the SV40 large T antigen; categorized under risk safety group S1 according to European legislation) |
| Applications | 1. Highly cited human cell models for investigating prostate growth, tissue architecture, and the mechanisms of benign proliferation; 2. Deciphering early events in malignant transformation and carcinogenesis using tissue reconstitution models; 3. Assessing dynamic changes in androgen and estrogen metabolic pathways (e.g., the role of aromatase); 4. Investigating stromal-epithelial interactions and paracrine signaling within the prostate microenvironment; 5. Screening for targeted therapeutic agents for lower urinary tract symptoms and the clinical progression of benign prostatic hyperplasia (BPH). |
| Shipped In | Dry ice |
| Storage Temperature | −196°C (Liquid nitrogen vapor phase) |
| Characteristics | |
| Tumorigenic | No, non-tumorigenic in nude or immunocompromised mice under standard isolation baselines. |
| Karyotype | The human karyotype is hypertriploid, with a polyploid proportion of 2.5%; the modal chromosome number fluctuates around 75 (69–79), and the karyotype is <3n>XXY. |
| Genetic Profile | 1. SV40 Transduction: Expresses functional SV40 Large T-antigen. 2. Hormone Receptor Status: Androgen Receptor (AR) negative; Prostate-Specific Antigen (PSA) negative. 3. TP53 Status: Retains/expresses p53 proto-oncogene protein. 4. Microsatellite Status: Stable (MSS). |
| Lineage Markers | Expresses luminal epithelial marker intermediate filaments Cytokeratin 8, 18, and 19, but tests completely negative for basal Cytokeratin 14. |
| Metabolic Profile | It actively metabolizes prostatic androgens and exhibits elevated baseline levels of estradiol (E₂) and aromatase expression compared to typical prostate cancer cell lines. |
| Growth Kinetics | Doubling time is approximately 50 hours under recommended maintenance guidelines. |
| Mycoplasma Test | Negative |
| Culture Conditions and Handling | |
| Subculturing | 1. Aspirate and discard the spent complete culture medium from the culture vessel. 2. Gently rinse the cell monolayer with sterile, Ca^2+/Mg^2+-free PBS to remove any residual traces of serum. 3. Add 2.0 to 3.0 mL of Trypsin-EDTA solution, ensuring it completely covers the cell monolayer. 4. Critical Step: BPH-1 cells exhibit extremely strong adherence and are tightly packed. Standard, short-duration enzymatic digestion alone will not be sufficient to detach them. Please incubate at 37°C for at least 10 minutes to ensure the cells fully round up and disperse. 5. Add an equal volume of complete culture medium to neutralize the enzymatic reaction. 6. Centrifuge to harvest the cells, aspirate the supernatant, carefully resuspend the cells in fresh medium, and then seed them into a new culture vessel. |
| Medium Renewal | Every 2 to 3 days (split confluent flasks every 3 to 7 days) |
| Subcultivation Ratio | Split confluent cultures at a ratio of 1:3 to 1:8 |
| Culture Conditions | Atmosphere: Air, 95%; CO2, 5%; Temperature: 37°C |
| Cryopreservation | 90% Complete growth medium + 10% DMSO (or 50% Basal medium + 40% FBS + 10% DMSO) |
| Cat.No. | Product Name | Price |
|---|---|---|
| CSC-RR0331 | GFP Reporter Cell Line - BPH-1 | Inquiry |
| CSC-RR00627 | Luciferase Reporter Cell Line - BPH-1 | Inquiry |
| CSC-RR00628 | GFP/Luc Reporter Cell Line - BPH-1 | Inquiry |
| CSC-RO01439 | OVAL Stable Cell Line - BPH-1 | Inquiry |
| CSC-RO01692 | GFP/OVAL Stable Cell Line - BPH-1 | Inquiry |
| CSC-RO01945 | Luc/OVAL Stable Cell Line - BPH-1 | Inquiry |
| CSC-RO02198 | GFP/Luc/OVAL Stable Cell Line - BPH-1 | Inquiry |
The above is only part of a part of cell line products. If you don't find the cell line you want, Creative Biogene can also provide stable cell line generation service with the best prices and fastest turnaround time for you! Contact us for more information or to request a quote.
Copyright © 2026 Creative Biogene. All rights reserved.