Luciferase Reporter Systems Make Tumor Research Twice as Easy

While it is true that some tumors or diseases are due to alterations in the genetic information of the genome itself, epigenetic regulation can sometimes better elucidate the mechanisms of tumor or disease development. As the subject progresses, especially after the phenotype has been identified, we tend to look at the mechanisms of phenogenesis in the direction of epigenetic regulation, or transcriptional regulation, or protein modification. The relationship between the differential gene and the phenotype is often relatively easy to determine, and the mining of the mechanism is relatively complex! Luciferase reporter is an assay that detects the interaction of transcription factors with DNA in the promoter region of target genes. As a mechanism research tool, if we want to study whether a certain transcription factor can have interaction with a certain target promoter fragment. First, the target gene promoter or other regulatory elements of the gene to be studied are inserted into the front of the luciferase reporter gene to construct a reporter gene plasmid. Then, the cells are co-transfected with the reporter gene plasmid, which can express the transcription factor to be detected; if this transcription factor can activate the target promoter, the luciferase gene will be expressed, and the amount of luciferase expression is directly proportional to the strength of the transcription factor's action; then, a specific luciferase substrate is added, and the luciferase reacts with the substrate to produce fluorescence. The intensity of the fluorescence can be detected to determine the activity of luciferase, so as to determine whether the transcription factor can have an effect with this target promoter fragment.

Introduction of Luciferase Reporter System

Luciferase reporter system (Luciferase reporter) is an assay to detect the interaction of transcription factors with DNA in the promoter region of target genes. As a mechanism research tool, if we want to study whether a certain transcription factor can have interaction with a certain target promoter fragment. First, the target gene promoter or other regulatory elements of the gene to be studied are inserted into the front of the luciferase reporter gene to construct a reporter gene plasmid. Then, the cells are co-transfected with the reporter gene plasmid, which can express the transcription factor to be detected; if this transcription factor can activate the target promoter, the luciferase gene will be expressed, and the amount of luciferase expression is directly proportional to the strength of the transcription factor's action; then, a specific luciferase substrate is added, and the luciferase reacts with the substrate to produce fluorescence. The intensity of the fluorescence can be detected to determine the activity of luciferase, so as to determine whether the transcription factor can have an effect with this target promoter fragment.

Fig. 1 Construction of stable transluciferase (Luc) gene tumor cell lines.

Application of Luciferase Reporter System

Functional studies of regulatory elements (promoters, enhancers, etc.)

Transcription factors, also known as trans-acting factors, can bind to specific sequences in their target promoters to regulate gene expression. These sequences in the target promoters are called cis-acting elements, and the two covalently bind to each other to inhibit or enhance gene expression. The reporter gene needs to be constructed upstream of the target promoter sequence. Co-transfection of the reporter gene plasmid with the plasmid expressing the transcription factor to be detected can be used to quantitatively detect the role of this type of transcription factor in regulating the target promoter through the change of fluorescence value directly.

miRNA target site research

miRNAs, or microRNAs, can fulfill their biological functions by participating in the regulation of their downstream gene translation processes. It is known that all animal miRNAs act on mRNA targets in their 3' UTR region. miRNAs usually bind to the RNA target of the target gene through its seed region, and 2-8 bases at the 5' end to match exactly with the target gene's RNA target, and induce gene repression by cleavage of the target transcription product. Constructing the miRNA's target 3' UTR destination gene downstream of the reporter gene can be used to verify the direct targeting relationship between the miRNA and the target gene.

Signaling pathway research

Classical ADCC/ADCP effects can also be studied by reporter gene method. Classical assays use donor's peripheral blood mononuclear cells PBMC or natural killer cells NK as effector cells, which are difficult to obtain, difficult to prepare, and unstable, which strongly affects their use as an active assay in the development and production of antibody drugs. Genetically engineered cells can be constructed to detect gene transcription mediated by the signaling pathway of NFAT (activated T-cell factor), which occurs first during the activation of the ADCC mechanism of action pathway, as an indicator. For example, using genetically engineered Jurkat cells as effector cells, which stably express the FcrRIIIa receptor (mutant expressing V158 high affinity or F158 low affinity), and the Luciferase gene whose expression is driven by the NFAT response element, the luciferase activity generated by the activation of the NFAT pathway can be quantitatively assayed Function of therapeutic antibody Fc effectors.

Luciferase Reporter Cell Lines

Luciferase reporter cell lines are engineered cell lines that express the luciferase enzyme, which can produce light when it interacts with specific substrates. These reporter cell lines are widely used in various fields of research, including molecular biology, drug discovery, and biotechnology. Creative Biogene scientists have established stable luciferase reporter gene cells in many popular cell lines suitable for various applications based on years of experience and in-depth research.

Cat.No.	Product Name	Price
CSC-RR0041	Luciferase Reporter Stable Cell Line-HEK293	Inquiry
CSC-RR0049	Luciferase Reporter Stable Cell Line-MCF-7	Inquiry
CSC-RR0061	Luciferase Reporter Stable Cell Line-SKOV-3	Inquiry
CSC-RR00610	Luciferase Reporter Cell Line - Farage	Inquiry
CSC-RR0222	TCF/LEF-Luc Reporter Cell Line-HEK293T	Inquiry
CSC-RR0224	Luc Reporter Cell Line-4T1	Inquiry
CSC-RR0234	Luc Reporter Cell Line-B16F10	Inquiry
CSC-RR0237	Luc Reporter Cell Line-CT26	Inquiry
CSC-RR0245	Luc Reporter Cell Line-LL/2(LLC)	Inquiry
CSC-RR0251	Luc Reporter Cell Line-A375	Inquiry

View more of our luciferase reporter cell lines products!

By introducing these luciferase reporter cell lines into your experiments, you can gain valuable insights into the mechanisms underlying various biological processes. For example, you can assess the activity of specific promoters by measuring luciferase expression levels, or you can monitor the activation of signaling pathways by examining changes in luciferase activity. Additionally, luciferase reporter cell lines can be utilized in high-throughput screening assays to identify potential drug candidates or to evaluate the effects of various compounds on cellular processes. The quantitative nature of luciferase readouts allows for precise and reliable measurements, making these cell lines valuable tools in drug discovery and development. Kinase Stable Cell Lines.

* For research use only. Not intended for any clinical use.

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Luciferase Reporter Systems Make Tumor Research Twice as Easy

Introduction of Luciferase Reporter System

Application of Luciferase Reporter System

Luciferase Reporter Cell Lines

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