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Frequently Asked Questions: LAM PCR Service

  1. What is the LAM PCR?
  2. What are the advantages of multiarm LAM PCR compared to standard single-arm LAM-PCR?
  3. How long does it take to finish the full service?
  4. What forms of sample do you accept (frozen cells or purified DNA)?
  5. Would it be possible to send you frozen cells and you isolated the DNA?

1. What is the LAM PCR?
Answer: LAM–PCR is short for linear - amplification mediated PCR. It is a technology which is used for identifying and characterizing unknown flanking DNA adjacent to known DNA of any origin. Recently, LAM-PCR has been developed to localize viral vector integration sites (IS) within the host genome.

2. What are the advantages of multiarm LAM PCR compared to standard single-arm LAM-PCR?
Answer:Firstly, multiarm LAM-PCR yields data at a higher rate than the individual arms, regardless of the sampling size. Secondly, multiarm LAM-PCR uses more than one restriction enzymes to digest the double strand DNA which decreases the detection bias resulting from the choice of restriction sites used to anchor the PCR. Thirdly, multiarm LAM PCR introduces an additional digest step which can eliminate the internal control band and a major source of noninformative clones in the junction library.

3. How long does it take to finish the full service?
Answer: Creative Biogene can provide the fastest turnaround time of any supplier in the industry. But the exact lead time depends on your detailed information and request of the project.

4. What forms of samples do you accept (frozen cells or purified DNA)?
Answer: Compared to other forms of samples, we prefer the purified DNA. Since the purified DNA would be much more stable than tissue or blood samples.

5. Would it be possible to send you frozen cells and you isolated the DNA?
Answer: Yes, we do purify DNA from blood and tissue samples, but this service needs an extra cost. You can get the detailed quotation of the full service by sending inquiry Email to us.

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