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| General Information | |
| Organism | Homo sapiens, human |
| Cell Line Description | SK-LU-1 is a human lung adenocarcinoma cell line established at the Memorial Sloan Kettering Cancer Center. It was derived from a primary lung tumor resected from a 60-year-old Caucasian female patient and is characterized as poorly differentiated (grade III). The cells grow as an adherent epithelial monolayer and have been extensively utilized in the National Cancer Institute’s (NCI) drug-screening platform, as well as in pharmacological research on non-small cell lung cancer (NSCLC). Genetically, SK-LU-1 harbors a heterozygous KRAS p.G12D activating mutation, a homozygous TP53 p.H193R hotspot mutation, and an ARID1A frameshift mutation that results in a truncated protein; the EGFR gene remains wild-type. The cell line exhibits the alternative lengthening of telomeres (ALT) mechanism, possesses a subtetraploid karyotype, and contains multiple marker chromosomes. SK-LU-1 is tumorigenic in immunotolerant rats and is classified as microsatellite stable (MSS). It is widely used in research concerning the biology of KRAS-driven NSCLC, high-throughput pharmacological screening, CRISPR-based functional genomic screening, and the assessment of drug responses in in vivo xenograft models. |
| Tissue | Lung (primary tumor, in situ) |
| Disease | Lung Adenocarcinoma; grade III, poorly differentiated |
| Morphology | Epithelial |
| Gender | Female |
| Age | 60 years |
| Ethnicity | Caucasian |
| Product Format | Frozen |
| Growth Mode | Adherent |
| Biosafety Level | 1 (Biosafety classification is based on U.S. Public Health Service Guidelines) |
| Applications | 1. KRAS-mutant non-small-cell lung cancer biology and signaling pathway analysis 2. Anti-cancer drug screening and pharmacogenomic studies 3. Investigation of p53 and ARID1A tumor-suppressor loss in lung adenocarcinoma 4. Telomere biology and alternative lengthening of telomeres (ALT) research 5. Tumorigenicity and chemotherapy studies in immunotolerant rats 6. Comparative oncology and in vivo therapeutic efficacy studies |
| Shipped In | Dry ice |
| Storage Temperature | −196°C |
| Characteristics | |
| Tumorigenic | Yes, forms tumors in immunotolerant rats |
| Karyotype | Hypotetraploid; modal chromosome number near 90 with multiple marker chromosomes. Loss of normal chromosome 15; disomic X; no Y chromosome detected |
| Genetic Profile | KRAS p.G12D (c.35G>A), heterozygous; TP53 p.H193R (c.578A>G), homozygous; ARID1A p.N1253fs*16 (c.3753delC), heterozygous |
| Expression Markers | Blood type O; Rh+; serological HLA Aw24, Aw32, B27, Bw41 (molecular HLA-A*24:02, B*40:02, C*02:02) |
| Mycoplasma Test | Negative |
| Culture Conditions and Handling | |
| Subculturing | 1. Remove and discard the culture medium. 2. Briefly rinse the cell monolayer with PBS (Ca²⁺/Mg²⁺-free) to thoroughly remove residual serum. 3. Add 2.0 to 3.0 mL of 0.25% Trypsin–0.53 mM EDTA solution, and observe under an inverted microscope until the cell monolayer has dispersed (typically takes 2 to 5 minutes). 4. Add 6.0 to 8.0 mL of complete growth medium, and gently pipette to prepare a single-cell suspension. 5. Aliquot the cell suspension into new culture vessels. |
| Medium Renewal | 2 times per week |
| Subcultivation Ratio | A split ratio of 1:2 to 1:4 is recommended when the monolayer reaches 70–80% confluence. |
| Culture Conditions | Atmosphere: Air, 95%; CO2, 5%; Temperature: 37°C |
| Cryopreservation | Complete growth medium supplemented with 5% (v/v) DMSO |
| Cat.No. | Product Name | Price |
|---|---|---|
| CSC-RR0285 | GFP Reporter Cell Line - SK-LU-1 | Inquiry |
| CSC-RO01558 | OVAL Stable Cell Line - SK-LU-1 | Inquiry |
| CSC-RO01811 | GFP/OVAL Stable Cell Line - SK-LU-1 | Inquiry |
| CSC-RO02064 | Luc/OVAL Stable Cell Line - SK-LU-1 | Inquiry |
| CSC-RO02317 | GFP/Luc/OVAL Stable Cell Line - SK-LU-1 | Inquiry |
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