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| General Information | |
| Organism | Homo sapiens, human |
| Cell Line Description | KYSE-180 is a human esophageal squamous cell carcinoma (ESCC) cell line established by Yutaka Shimada and colleagues at the First Department of Surgery, Kyoto University Faculty of Medicine. The cell line was derived from a primary tumor located in the mid-thoracic esophagus of a 53-year-old Japanese male patient, who was diagnosed with a well-differentiated ESCC (stage T4N1M0). These cells grow as an adherent monolayer and exhibit an epithelial-like morphology; they have a doubling time of approximately 40 to 50 hours and are tumorigenic in immunodeficient mice. KYSE-180 harbors a homozygous TP53 p.Ile195Thr mutation and exhibits CCND1 (cyclin D1) amplification. The cell line has a hyperdiploid karyotype with a modal chromosome number of 55 and is microsatellite-stable. It is widely used in ESCC research, including studies on radiosensitivity, autocrine nerve growth factor signaling, chromosomal instability and LRP1B, combined radiotherapy and anti-angiogenic therapy, as well as cancer pharmacogenomics and CRISPR-based screening. |
| Tissue | Esophagus, middle intra-thoracic segment (primary tumor, in situ) |
| Disease | Esophageal Squamous Cell Carcinoma (ESCC); well differentiated; T4N1M0, stage 3 |
| Morphology | Epithelial-like |
| Gender | Male |
| Age | 53 years |
| Ethnicity | Japanese |
| Product Format | Frozen |
| Growth Mode | Adherent |
| Biosafety Level | 1 (Biosafety classification is based on U.S. Public Health Service Guidelines) |
| Applications | 1. Research on the biology and tumor progression of esophageal squamous cell carcinoma; 2. Analysis of radiosensitivity profiles in a panel of esophageal cancer cell lines; 3. Investigation of nerve growth factor autocrine loops and neurotrophic factor signaling pathways; 4. Study of chromosomal instability, allelic imbalance, and LRP1B gene silencing; 5. Evaluation of combined treatment using radiotherapy and anti-angiogenic agents; 6. Pharmacogenomic profiling and CRISPR-based functional screening. |
| Shipped In | Dry ice |
| Storage Temperature | −196°C |
| Characteristics | |
| Tumorigenic | Yes, xenotransplantation positive in immunodeficient mice |
| Karyotype | Human hyperdiploid; modal number 55 (range 50–57) <2n>XX with 11% polyploidy and multiple structural abnormalities including del(X)(q23), del(6)(q23), del(11)(q11q14) and i(7q) |
| Genetic Profile | TP53 p.Ile195Thr (c.584T>C), homozygous; cyclin D1 (CCND1) amplification |
| Mycoplasma Test | Negative |
| Viral Status | Reverse transcriptase negative by ELISA; PCR negative for EBV, HBV, HCV, HHV-8, HIV-1, HIV-2, HPV, HTLV-1, HTLV-2, MLV and SMRV |
| Culture Conditions and Handling | |
| Subculturing | 1. Remove and discard the culture medium. 2. Briefly rinse the cell monolayer with PBS (Ca²⁺/Mg²⁺-free) to thoroughly remove residual serum. 3. Add 2.0 to 3.0 mL of 0.25% Trypsin–0.53 mM EDTA solution, and observe under an inverted microscope until the cell monolayer has dispersed (typically takes 2 to 5 minutes). 4. Add 6.0 to 8.0 mL of complete growth medium, and gently pipette to prepare a single-cell suspension. 5. Aliquot the cell suspension into new culture vessels. |
| Medium Renewal | Every 3 to 5 days |
| Subcultivation Ratio | Split confluent cultures at a ratio of 1:5 to 1:10; seed at approximately 2–3 × 10⁶ cells per 80 cm². |
| Culture Conditions | Atmosphere: Air, 95%; CO2, 5%; Temperature: 37°C |
| Cryopreservation | 70% complete growth medium + 20% FBS + 10% DMSO |
| Cat.No. | Product Name | Price |
|---|---|---|
| CSC-RR0343 | GFP Reporter Cell Line - KYSE-180 | Inquiry |
| CSC-RO01501 | OVAL Stable Cell Line - KYSE-180 | Inquiry |
| CSC-RO01754 | GFP/OVAL Stable Cell Line - KYSE-180 | Inquiry |
| CSC-RO02007 | Luc/OVAL Stable Cell Line - KYSE-180 | Inquiry |
| CSC-RO02260 | GFP/Luc/OVAL Stable Cell Line - KYSE-180 | Inquiry |
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