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| General Information | |
| Organism | Homo sapiens, human |
| Cell Line Description | CAL-51 is a human breast adenocarcinoma cell line derived from the malignant pleural effusion of a 45-year-old female patient with metastatic breast cancer. CAL-51 cells exhibit an epithelial morphology and grow as an adherent monolayer under standard in vitro culture conditions. Phenotypically, CAL-51 serves as a model for triple-negative breast cancer (TNBC), characterized by the absence of estrogen receptor (ER) and progesterone receptor (PR) expression, as well as the lack of human epidermal growth factor receptor 2 (HER2/ERBB2) overexpression. Genetically, the cell line harbors alterations in key oncogenic driver genes, including mutations in PIK3CA and TP53. Due to its triple-negative phenotype and distinct molecular profile, CAL-51 is widely utilized in translational breast cancer research; it serves as a valuable model for investigating TNBC pathogenesis, evaluating novel targeted therapies and chemotherapeutic agents, analyzing synthetic lethal interactions, and conducting high-throughput genomic or pharmacological screens. |
| Tissue | Breast; derived from metastatic site (Pleural effusion) |
| Disease | Breast Adenocarcinoma; Triple-Negative Breast Cancer (TNBC) |
| Morphology | Epithelial |
| Gender | Female |
| Age | 45 years |
| Product Format | Frozen |
| Growth Mode | Adherent |
| Biosafety Level | 1 (Biosafety classification is based on U.S. Public Health Service Guidelines) |
| Applications | 1. Research on the biological characteristics and metastatic mechanisms of triple-negative breast cancer (TNBC); 2. In vitro screening and evaluation of novel chemotherapy and targeted drug candidates; 3. Research on synthetic lethality and PI3K pathway inhibition; 4. Genome-wide functional knockout and high-throughput pharmacological screening. |
| Shipped In | Dry ice |
| Storage Temperature | −196°C |
| Characteristics | |
| Karyotype | Aneuploid |
| Tumorigenic | Yes, tumorigenic in immunocompromised mice |
| Genetic Profile | PIK3CA mutation; TP53 mutation; ER negative (ER-); PR negative (PR-); HER2 negative (HER2-) |
| Mycoplasma Test | Negative |
| Culture Conditions and Handling | |
| Subculturing | 1. Remove and discard the culture medium. 2. Briefly rinse the cell monolayer with PBS (without Ca2+/Mg2+) to completely remove residual serum. 3. Add 1.0 to 2.0 mL of 0.25% trypsin-0.03% EDTA solution and incubate at 37°C until the cell monolayer detaches (typically 2 to 5 minutes). 4. Add fresh complete growth medium to neutralize the trypsin and gently pipette up and down to prepare a single-cell suspension. 5. Subculture the cell suspension into new culture vessels. |
| Medium Renewal | Every 2 to 3 days |
| Subcultivation Ratio | A split ratio of 1:3 to 1:6 is recommended |
| Culture Conditions | Atmosphere: Air, 95%; CO2, 5%; Temperature: 37°C |
| Cryopreservation | Complete growth medium supplemented with 5% to 10% (v/v) DMSO |
| Cat.No. | Product Name | Price |
|---|---|---|
| CSC-RR0333 | GFP Reporter Cell Line - CAL-51 | Inquiry |
| CSC-RO01450 | OVAL Stable Cell Line - CAL51 | Inquiry |
| CSC-RO01703 | GFP/OVAL Stable Cell Line - CAL51 | Inquiry |
| CSC-RO01956 | Luc/OVAL Stable Cell Line - CAL51 | Inquiry |
| CSC-RO02209 | GFP/Luc/OVAL Stable Cell Line - CAL51 | Inquiry |
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