Our promise to you:
Guaranteed product quality, expert customer support.
24x7 CUSTOMER SERVICE
CONTACT US TO ORDER
| General Information | |
| Organism | Homo sapiens, human |
| Cell Line Description | Panc 10.05 is a human pancreatic adenocarcinoma cell line established from a primary tumor located in the head of the pancreas. The cells display epithelial morphology and form an adherent monolayer under standard culture conditions. The model carries clinically relevant alterations in KRAS and TP53 and retains epithelial marker expression, making it well suited to studies of pancreatic tumor biology, oncogenic signaling, treatment response, and three-dimensional growth. Its defined molecular background also supports comparative work with other pancreatic cancer models and genetically stratified experimental designs. |
| Tissue | Pancreas; primary tumor from the head of the pancreas |
| Disease | Pancreatic adenocarcinoma; classified as pancreatic ductal adenocarcinoma |
| Morphology | Epithelial |
| Sex | Male |
| Age at Sampling | 81 years |
| Population | White / Caucasian |
| Year of Origin | 1992 |
| Product Format | Frozen |
| Growth Mode | Adherent |
| Biosafety Level | 1 |
| Applications | 1. Pancreatic adenocarcinoma biology and disease-mechanism research 2. Investigation of KRAS-driven signaling and TP53-associated cellular phenotypes 3. Evaluation of anticancer compounds, pathway inhibitors, and combination strategies 4. Three-dimensional culture, tumor growth, and cell-survival studies 5. Genomic, transcriptomic, proteomic, and functional screening workflows 6. Comparative studies using molecularly defined pancreatic cancer models |
| Storage Conditions | Store below −130°C; vapor-phase liquid nitrogen is recommended for long-term preservation |
| Characteristics | |
| Tumorigenic | Yes; capable of forming tumors in immunodeficient mice |
| Genetic Profile | KRAS p.Gly12Asp (G12D; c.35G>A) and TP53 p.Ile255Asn (I255N; c.764T>A) variants have been reported. KRAS G12D provides a defined background for examining RAS-pathway activation, while the TP53 alteration enables investigation of p53-associated tumor phenotypes. |
| Antigen Expression | MHC class I positive; MHC class II negative |
| Epithelial Markers | Cytokeratin 7 and cytokeratin 18 are expressed |
| Population Doubling Time | Approximately 19.2 hours under the reported test conditions. A value of approximately 30 hours has also been reported, and actual growth rate may vary with medium preparation, passage history, seeding density, and laboratory conditions. |
| Plating Efficiency | Reported plating efficiency: approximately 40% |
| Microsatellite Status | Microsatellite stable (MSS) |
| Mycoplasma Test | Negative |
| Culture Conditions and Handling | |
| Culture Conditions | 37°C in a humidified atmosphere containing 5% CO₂ in air |
| Subculturing | 1. Remove the spent medium from the culture vessel. 2. Briefly rinse the cell layer with 0.25% (w/v) trypsin–0.53 mM EDTA to remove residual serum. 3. Add 2.0-3.0 mL of trypsin-EDTA per 75 cm² flask and monitor the culture microscopically until the monolayer disperses, typically within 5-15 minutes. Avoid striking or vigorously shaking the vessel during detachment. 4. Add 6.0–8.0 mL of complete medium and gently pipette to obtain an even cell suspension. 5. Transfer the required aliquot to a new culture vessel and return the culture to 37°C. |
| Subcultivation Ratio | A split ratio of 1:2 to 1:4 is recommended |
| Medium Renewal | Replace the medium every 2 to 3 days |
| Thawing and Recovery | Thaw the vial rapidly with gentle agitation in a 37°C water bath, usually within approximately 2 minutes. Transfer the contents to 9.0 mL of complete medium and centrifuge at approximately 125 × g for 5-7 minutes. Resuspend the pellet in fresh complete medium and seed into a suitable culture flask. Pre-equilibrating the medium helps reduce pH stress during recovery. |
| Cryopreservation | Complete growth medium supplemented with 5% (v/v) DMSO |
| Handling Notes | Maintain strict aseptic technique throughout recovery and routine culture. Avoid prolonged exposure to trypsin, excessive mechanical force, and unnecessary alkalinization of the medium. Monitor morphology, confluence, and contamination status regularly; optimize seeding density and passage timing for the intended assay. |
| Cat.No. | Product Name | Price |
|---|---|---|
| CSC-RO0221 | Cas9 Stable Cell Line - Panc 10.05 | Inquiry |
| CSC-RR0508 | GFP/Luc Reporter Cell Line - Panc 10.05 | Inquiry |
| CSC-RO01634 | OVAL Stable Cell Line - Panc 10.05 | Inquiry |
| CSC-RO01887 | GFP/OVAL Stable Cell Line - Panc 10.05 | Inquiry |
| CSC-RO02140 | Luc/OVAL Stable Cell Line - Panc 10.05 | Inquiry |
| CSC-RO02393 | GFP/Luc/OVAL Stable Cell Line - Panc 10.05 | Inquiry |
The above is only part of a part of cell line products. If you don't find the cell line you want, Creative Biogene can also provide stable cell line generation service with the best prices and fastest turnaround time for you! Contact us for more information or to request a quote.
Copyright © 2026 Creative Biogene. All rights reserved.