SCC-9 Cell Line

Brief Introduction
Product List

General Information
Organism	Homo sapiens, human
Cell Line Description	SCC-9 is a human squamous cell carcinoma cell line derived from the tongue of a 25-year-old male patient. The cells display an epithelial morphology, grow adherently in culture, and are widely utilized in head and neck cancer research, particularly for studying oral cavity squamous cell carcinoma biology. SCC-9 cells exhibit gene expression of epidermal keratins as well as low levels of involucrin. They harbor genomic alterations, including mutations in the TP53 tumor suppressor gene. The line demonstrates robust tumorigenic capacity, forming tumours within 21 days at high frequency when inoculated subcutaneously into immunocompromised mice. While growth can be enhanced using feeder layers under specific conditions, SCC-9 cells generally do not grow well in semi-solid media. This line serves as a valuable model for investigating oral oncogenesis, evaluating novel chemotherapeutic agents, and conducting target identification through genome-wide screening strategies.
Tissue	Tongue
Disease	Squamous Cell Carcinoma
Morphology	Epithelial
Gender	Male
Age	25 years
Product Format	Frozen
Growth Mode	Adherent
Biosafety Level	1 (Biosafety classification is based on U.S. Public Health Service Guidelines)
Applications	1. Research on the biology and mechanisms of tumorigenesis in oral squamous cell carcinoma (OSCC) 2. In vitro screening and evaluation of candidate chemotherapeutic or targeted drugs 3. Establishment of nude mouse tumor xenograft models 4. Genome-wide CRISPR/Cas9 functional knockout screening
Shipped In	Dry ice
Storage Temperature	−196°C
Characteristics
Karyotype	Aneuploid
Tumorigenic	Yes, tumors develop within 21 days at 100% frequency (5/5) in nude mice inoculated subcutaneously with 10⁷ cells
Genetic Profile	TP53 mutation; CDKN2A alteration
Expression Markers	Expresses epidermal keratins; low levels of involucrin
Mycoplasma Test	Negative
Culture Conditions and Handling
Subculturing	1. Remove and discard the culture medium. 2. Briefly rinse the cell monolayer with PBS (without Ca2+/Mg2+) to completely remove residual serum. 3. Add 1.0 to 2.0 mL of 0.25% trypsin-0.03% EDTA solution and incubate the culture vessel at room temperature or 37°C until the cell monolayer detaches. 4. Add fresh complete medium to neutralize the trypsin and gently pipette up and down to prepare a single-cell suspension. 5. Subculture the cell suspension into new culture vessels.
Medium Renewal	Every 2 to 3 days
Subcultivation Ratio	A split ratio of 1:3 to 1:6 is recommended
Culture Conditions	Atmosphere: Air, 95%; CO2, 5%; Temperature: 37°C
Cryopreservation	Complete growth medium supplemented with 5% to 10% (v/v) DMSO (or 90% FBS + 10% DMSO)

Cat.No.	Product Name	Price
CSC-RT0114	NGFR Knockout Cell Line-SCC-9	Inquiry

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* For research use only. Not intended for any clinical use.

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