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| General Information | |
| Organism | Chlorocebus aethiops, African green monkey |
| Cell Line Description | COS-1 is an adherent, fibroblast-like nonhuman primate kidney cell line derived from CV-1 cells by transformation with an origin-defective SV40 mutant. The cells contain a single integrated copy of the complete SV40 early region and constitutively express wild-type T antigen. This background enables transfection with vectors that require SV40 T-antigen activity and supports lytic SV40 growth and propagation of early-region deletion mutants. COS-1 is therefore suited to transient gene-expression, host–vector, viral replication, protein-function, and three-dimensional culture studies. |
| Cell Type | SV40-transformed kidney-derived fibroblast-like cell |
| Tissue of Origin | Kidney |
| Disease | No associated disease |
| Sex | Male |
| Age at Sampling | Adult |
| Parent Cell Line | CV-1 |
| Morphology | Fibroblast-like |
| Growth Mode | Adherent monolayer |
| Product Format | Frozen |
| Applications | 1. Transient transfection and heterologous gene-expression studies 2. Vectors requiring SV40 T-antigen activity 3. SV40 replication, mutant complementation, and virus–host interaction studies 4. Propagation of recombinant SV40 populations 5. Mammalian host–vector system development 6. Protein trafficking, receptor, signaling, and promoter-function assays 7. Three-dimensional cell culture 8. Cytotoxicity and cellular uptake studies 9. Nonhuman primate kidney cell biology 10. Comparative cell-cycle and transformation research with explicit SV40-related controls |
| Storage Conditions | Store below −130°C, preferably in the vapor phase of liquid nitrogen |
| Characteristics | |
| Transformation Method | Transformation of CV-1 cells with the pSV6-1 origin-defective SV40 mutant encoding wild-type T antigen |
| Integrated Viral Sequence | A single integrated copy of the complete SV40 early region |
| Replication-Origin Defect | The transforming SV40 construct contains a 6 bp deletion in the replication origin, preventing autonomous replication of the integrated construct. |
| Expressed Viral Protein | SV40 T antigen |
| Additional Marker Profile | EBNA, Fc receptors, and complement receptors are negative in the referenced characterization. |
| Virus Susceptibility | Permissive for lytic SV40 growth; supports ts A209 replication at 40°C and propagation of SV40 mutants containing deletions in the early region |
| Karyotype | Hypotetraploid simian karyotype with structural rearrangements: modal chromosome count 102, range 95–108, with approximately 4% polyploid cells in the referenced cytogenetic analysis |
| Population Doubling Time | Approximately 48 hours under the referenced culture conditions; growth rate can vary with passage, inoculum, serum, and laboratory conditions. |
| Mycoplasma Test | Negative |
| Culture Conditions and Handling | |
| Culture Conditions | 37°C in a humidified atmosphere containing 5% CO₂ |
| Medium Renewal | Replace the medium 2–3 times per week |
| Subcultivation Ratio | A split ratio of 1:4 to 1:8 is recommended for the detailed handling protocol |
| Dissociation Reagent | 0.25% (w/v) trypsin with 0.53 mM EDTA |
| Subculturing | 1. Remove and discard the culture medium. 2. Briefly rinse the cell layer with 0.25% trypsin–0.53 mM EDTA to remove residual serum. 3. Add 2.0–3.0 mL dissociation solution per 75 cm² flask. 4. Observe under an inverted microscope until the monolayer disperses, typically within 5–10 minutes. Do not strike or shake the flask while waiting; place difficult cultures at 37°C to assist detachment. 5. Add 6.0–8.0 mL complete growth medium and gently pipette to obtain a uniform suspension. 6. Dispense the suspension into new vessels at a 1:4 to 1:8 split ratio. 7. Incubate at 37°C and 5% CO₂. |
| Thawing and Recovery | Thaw rapidly with gentle agitation in a 37°C water bath for approximately 2 minutes. Decontaminate the vial exterior with 70% ethanol and continue aseptically. Transfer the contents to 9.0 mL complete medium and centrifuge at approximately 125 × g for 5–7 minutes. Discard the supernatant, resuspend the pellet in complete medium, seed a 25 cm² flask, and incubate at 37°C and 5% CO₂. Pre-equilibrating the medium for at least 15 minutes helps restore the recommended pH range of 7.0–7.6. |
| Cryopreservation Medium | Complete growth medium supplemented with 5% (v/v) DMSO |
| Confluence Control | Maintain cultures below full confluence. Overconfluent cultures can form syncytia. |
| Handling Notes | Standardize passage number, seeding density, serum lot, confluence, and time after transfection across experiments. COS-1 can amplify SV40-origin-dependent constructs through constitutive T-antigen activity, so include vector-only and replication-competent controls when copy number or expression level affects interpretation. Verify species identity and mycoplasma status periodically. |
| Cat.No. | Product Name | Price |
|---|---|---|
| CSC-RR0090 | tGFP Reporter Stable Cell Line-COS-1 | Inquiry |
| CSC-RR00752 | Luciferase Reporter Cell Line - cos-1 | Inquiry |
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