T7E1 Assay Protocol
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T7E1 Assay Protocol
| Delivery methods: Lentivirus Transduction Plasmid transfection Ribonucleoprotein delivery |
| Please following the procedure in Genomic Extraction Kit Or using the Direct PCR Kit to treat cell samples |
| Nest PCR First round PCR: 20 cycles 800-1000bp length Second round PCR: 30-40cycles Around 500bp length |
| Annealing procedure  |
| Please following the procedure in Knockout Detection Kit Or using T7E1 enzyme for incubation in 37℃ for 30min |
| Data shall be visualized by 1.2%-1.5% agarose gel |
Step1：CRISPR Delivery
Step2：Genome Extraction
Step3：Nest PCR for Target site
Step4：PCR product Annealing
Step5：T7E1 treatment
Step6：Gel electrophoresisNote: direct PCR product could be used for T7E1 detection.
After treated with T7E1, reactions should be electrophoresed by high concentration agarose gels.