Our promise to you:
Guaranteed product quality, expert customer support.
Please following the procedure in Genomic Extraction Kit
Or using the Direct PCR Kit to treat cell samples
Step3：Nest PCR for Target site
First round PCR: 20 cycles
Second round PCR: 30-40cycles
Around 500bp length
Step4：PCR product Annealing
Please following the procedure in Knockout Detection Kit
Or using T7E1 enzyme for incubation in 37℃ for 30min
|Data shall be visualized by 1.2%-1.5% agarose gel|
Note: direct PCR product could be used for T7E1 detection.
After treated with T7E1, reactions should be electrophoresed by high concentration agarose gels.
Creative Biogene is a US-based manufacturer and provider of genomics and proteomics products and services for academic and governmental research institutes, pharmaceutical and biotechnology industry.