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Please following the procedure in Genomic Extraction Kit
Or using the Direct PCR Kit to treat cell samples
Step3：Nest PCR for Target site
First round PCR: 20 cycles
Second round PCR: 30-40cycles
Around 500bp length
Step4：PCR product Annealing
Please following the procedure in Knockout Detection Kit
Or using T7E1 enzyme for incubation in 37℃ for 30min
|Data shall be visualized by 1.2%-1.5% agarose gel|
Note: direct PCR product could be used for T7E1 detection.
After treated with T7E1, reactions should be electrophoresed by high concentration agarose gels.