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| Delivery methods: Lentivirus Transduction Plasmid transfection Ribonucleoprotein delivery |
| Please following the procedure in Genomic Extraction Kit Or using the Direct PCR Kit to treat cell samples |
| Nest PCR First round PCR: 20 cycles 800-1000bp length Second round PCR: 30-40cycles Around 500bp length |
| Annealing procedure ![]() |
| Please following the procedure in Knockout Detection Kit Or using T7E1 enzyme for incubation in 37℃ for 30min |
| Data shall be visualized by 1.2%-1.5% agarose gel |
Note: direct PCR product could be used for T7E1 detection.
After treated with T7E1, reactions should be electrophoresed by high concentration agarose gels.
Creative Biogene is a US-based manufacturer and provider of genomics and proteomics products and services for academic and governmental research institutes, pharmaceutical and biotechnology industry.