266-6 Cell Line

Brief Introduction
Product List

General Information
Organism	Mus musculus, mouse
Cell Line Description	266-6 is a transformed mouse pancreatic acinar cell line derived from an acinar-cell tumor in an adult animal. The tumor was induced by pancreas-directed expression of SV40 large T antigen under control of an elastase I regulatory sequence. The cells grow as an adherent epithelial-like monolayer, retain a partially differentiated acinar phenotype, express multiple digestive-enzyme transcripts, and respond selectively to secretagogue stimulation. These properties make 266-6 a practical model for pancreatic acinar-cell biology, exocrine signaling, gene regulation, inflammatory and metabolic stress, pancreatic tumor research, and transfection-based functional studies.
Cell Type	Mouse pancreatic acinar cell
Tissue of Origin	Pancreas
Disease	Pancreatic acinar-cell tumor / neoplasm
Age at Sampling	Adult
Sex	Not reported
Year Established	1985
Morphology	Epithelial-like; round, irregular, and polygonal cells have been reported
Transformation	Tumor induction involved an elastase I/SV40 T-antigen fusion construct. The established cells also carry an elastase I/neomycin transgene.
Product Format	Frozen
Growth Mode	Adherent
Biosafety Level	Biosafety Level 2 is reported because the cells contain papovavirus/SV40-derived sequences; confirm local handling requirements through a risk assessment before use
Applications	1. Pancreatic acinar-cell differentiation and exocrine-function research 2. Digestive-enzyme expression and secretory-pathway studies 3. Cholecystokinin- and muscarinic receptor signaling assays 4. Pancreatic acinar-cell tumor biology and oncogenic-transformation research 5. Pancreatitis-associated inflammatory and cellular-stress models 6. Nutrient and vitamin transport, metabolism, and gene-regulation studies 7. Transfection, enhancer, promoter, and functional-genomics workflows 8. Three-dimensional pancreatic cell-culture studies and compound screening
Storage Conditions	Store below −130°C, preferably in the vapor phase of liquid nitrogen, for long-term preservation
Characteristics
Differentiation Status	Retains a partially differentiated pancreatic acinar-cell phenotype rather than a fully mature primary-acinar phenotype
Digestive-Enzyme Expression	Expresses detectable transcript levels for multiple digestive enzymes.
Receptor Expression	Muscarinic acetylcholine receptor expression has been reported
Secretagogue Response	Responds to carbachol and cholecystokinin under reported assay conditions
Nonresponsive Agonists	No response to substance P, secretin, or vasoactive intestinal peptide was reported in the original characterization context
Mycoplasma Test	Negative
Culture Conditions and Handling
Culture Conditions	37°C in a humidified atmosphere containing 5% CO₂
Culture Vessel Coating	Use tissue-culture vessels coated with 0.1% gelatin. Apply approximately 0.4–0.5 mL coating solution per cm², refrigerate for 10–15 minutes, remove the coating solution, rinse gently with complete medium, and use immediately. Prepared coated vessels may be stored at 2–8°C for no more than 5 days.
Recommended Seeding Density	Approximately 2–3 × 10⁴ viable cells/cm² is reported in one expansion protocol. Optimize for vessel format, coating quality, passage history, and experimental endpoint.
Medium Renewal	Replace the medium 2–3 times per week.
Passage Endpoint	Subculture before prolonged over-confluence. One handling protocol passages cultures at approximately 95% confluence; use a consistent endpoint for comparative experiments.
Subculturing	1. Remove the spent medium and briefly rinse the monolayer with 0.25% trypsin–0.53 mM EDTA to remove residual serum. 2. Add approximately 2–3 mL of trypsin–EDTA to a T-75 flask and monitor under an inverted microscope. 3. Incubate until the cell layer disperses, typically within 5–15 minutes; difficult cultures may be placed at 37°C. Avoid hitting or shaking the flask during detachment. 4. Add approximately 6–8 mL of complete medium and gently pipette to collect the cells. 5. Centrifuge at approximately 125 × g for 5–10 minutes to remove the dissociation reagent. 6. Discard the supernatant, gently resuspend the pellet in fresh complete medium, and seed into newly prepared 0.1% gelatin-coated vessels. 7. Return the cultures to 37°C and 5% CO₂.
Subcultivation Ratio	A split ratio of 1:3 to 1:4 is recommended in the reference protocol; a separate lot-specific protocol reports 1:2 to 1:4
Thawing and Recovery	Pre-equilibrate complete medium in the incubator for at least 15 minutes. Thaw the vial rapidly by gentle agitation in a 37°C water bath for approximately 2 minutes while keeping the cap above the waterline. Disinfect the exterior, then centrifuge at approximately 125 × g for 5–7 minutes to remove the cryoprotectant. Gently resuspend the pellet, seed into a 0.1% gelatin-coated T-75 flask with complete medium, and incubate at 37°C and 5% CO₂.
Cryopreservation	Use complete growth medium supplemented with 5% DMSO. Freeze at a controlled rate before transfer to the vapor phase of liquid nitrogen.

Cat.No.	Product Name	Price
CSC-RO01156	Cas9 Stable Cell Line - 266-6	Inquiry
CSC-RR00655	Luciferase Reporter Cell Line - 266-6	Inquiry
CSC-RO01419	OVAL Stable Cell Line - 266-6	Inquiry
CSC-RO01672	GFP/OVAL Stable Cell Line - 266-6	Inquiry
CSC-RO01925	Luc/OVAL Stable Cell Line - 266-6	Inquiry
CSC-RO02178	GFP/Luc/OVAL Stable Cell Line - 266-6	Inquiry

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* For research use only. Not intended for any clinical use.

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