HC11 Cell Line

Brief Introduction
Product List

General Information
Organism	Mus musculus, mouse
Cell Line Description	HC11 is a clonal mouse mammary epithelial cell line derived from the COMMA-D cell line (which originated from mid-pregnant BALB/c mouse mammary tissue). HC11 cells exhibit an epithelial morphology and grow as an adherent monolayer under standard in vitro culture conditions. A distinctive feature of this cell line is its retained capacity for functional differentiation in response to lactogenic hormones; upon combined induction with prolactin, dexamethasone, and insulin, HC11 cells rapidly differentiate and express milk protein genes, particularly the β-casein gene. Genetically, the cell line harbors a point mutation in the Trp53 gene that leads to immortalization, yet it maintains near-normal, non-tumorigenic biological characteristics under standard conditions. HC11 serves as a highly valuable and physiologically relevant model for studying mammary gland development, lactogenic signaling (such as the STAT5 pathway), mechanisms of hormone-regulated gene expression, and early events in mammary tumorigenesis.
Tissue	Mammary gland
Disease	Normal-derived immortalized cell line
Morphology	Epithelial
Gender	Female
Age	1 year
Product Format	Frozen
Growth Mode	Adherent
Biosafety Level	1 (Biosafety classification is based on U.S. Public Health Service Guidelines)
Applications	1. Research on mammary gland development and prolactin signaling (STAT5/JAK2 pathway); 2. Research on the regulation of milk protein gene expression (e.g., β-casein synthesis); 3. In vitro models for studying lactational differentiation and epithelial morphogenesis; 4. Functional analysis of the early stages of oncogenic transformation in mammary epithelial cells.
Shipped In	Dry ice
Storage Temperature	−196°C
Characteristics
Karyotype	Aneuploid
Tumorigenic	Non-tumorigenic under standard conditions; can form tumors upon specific oncogenic transformation
Genetic Profile	Trp53 point mutation
Expression Markers	Expresses cytokeratins; secretes beta-casein upon induction with prolactin, dexamethasone, and insulin
Mycoplasma Test	Negative
Culture Conditions and Handling
Subculturing	1. Remove and discard the culture medium. 2. Briefly rinse the cell monolayer with PBS (without Ca2+/Mg2+) to completely remove residual serum. 3. Add 1.0 to 2.0 mL of 0.25% trypsin-0.03% EDTA solution and incubate at 37°C until the cell monolayer detaches (typically 2 to 5 minutes). 4. Add fresh complete growth medium to neutralize the trypsin and gently pipette up and down to prepare a single-cell suspension. 5. Subculture the cell suspension into new culture vessels.
Medium Renewal	Every 2 to 3 days
Subcultivation Ratio	A split ratio of 1:3 to 1:6 is recommended
Culture Conditions	Atmosphere: Air, 95%; CO2, 5%; Temperature: 37°C
Cryopreservation	Complete growth medium supplemented with 5% to 10% (v/v) DMSO

Cat.No.	Product Name	Price
CSC-RO01340	Human Serp1 Stable Cell Line - HC11	Inquiry

The above is only part of a part of cell line products. If you don't find the cell line you want, Creative Biogene can also provide stable cell line generation service with the best prices and fastest turnaround time for you! Contact us for more information or to request a quote.

* For research use only. Not intended for any clinical use.

Quick Inquiry