MDBK Cell Line

Brief Introduction
Product List

General Information
Organism	Bos taurus, cow
Cell Line Description	MDBK is a continuous bovine kidney epithelial cell line established by S.H. Madin and N.B. Darby in 1957. The cell line was derived from normal kidney tissue of a healthy adult steer (a castrated male bovine). MDBK cells grow as an adherent monolayer with tight intercellular junctions, exhibiting a characteristic "cobblestone" epithelial morphology. MDBK is recognized as a cornerstone model system in the fields of veterinary medicine, virology, and biotechnology. It is widely used for the isolation, diagnostic propagation, and industrial-scale vaccine production of various important veterinary viruses, particularly bovine viral diarrhea virus (BVDV) and bovine herpesvirus type 1 (BHV-1). Additionally, MDBK serves as a common non-human host system for assaying interferon activity and studying viral replication kinetics.
Tissue	Kidney; derived from in situ renal parenchyma
Cell Type	Epithelial cell
Breed / Strain	Hereford
Disease	Normal / Non-malignant tissue origin (Spontaneously immortalized)
Morphology	Epithelial-like; uniform polygonal cells growing in tightly packed configurations
Gender	Male
Age	Adult
Product Format	Frozen
Growth Mode	Adherent
Biosafety Level	1 (Biosafety classification matches global standard laboratory parameters for non-infectious agricultural bioproducts)
Applications	1. Diagnostic isolation, propagation for research purposes, and commercial vaccine production for Bovine Herpesvirus 1 (BHV-1), Bovine Viral Diarrhea Virus (BVDV), and Vesicular Stomatitis Virus (VSV); 2. Standard target cell substrates for the quantitative comparative biological assay of human and bovine Type I interferons (IFN-α/β); 3. Modeling of renal epithelial cell transport mechanisms, dynamic changes in polarity, and membrane permeability kinetics; 4. Investigation of cytotoxicity, networks of environmental heavy metal exposure, and veterinary drug-induced nephrotoxicity; 5. Evaluation of adhesion mechanisms of livestock pathogens and their interactions with host cell structures.
Shipped In	Dry ice
Storage Temperature	−196°C (Liquid nitrogen vapor phase only)
Characteristics
Tumorigenic	No, non-tumorigenic under standard physiological baselines. Does not form tumors when engrafted into standard immunocompromised rodent hosts.
Transformant	None; immortalized via continuous spontaneous cell replication adaptation during historical serially passaged selection.
Viral Sensitivity Status	It is naturally highly susceptible to a variety of viruses, including bovine herpesvirus type 1, bovine viral diarrhea virus (BVDV), vesicular stomatitis virus (Indiana strain), vaccinia virus, and adenovirus.
Growth Kinetics	Rapid, highly robust adherent proliferation; typical population doubling time spans approximately 20 to 30 hours.
Mycoplasma Test	Negative
Culture Conditions and Handling
Subculturing	1. Remove and discard all used complete culture medium from the culture dish. 2. Gently rinse the confluent cell layer with sterile PBS or a 0.25% trypsin-0.53 mM EDTA solution (Ca2+/Mg2+-free) to neutralize residual serum. 3. Aspirate the rinse solution, add 2.0 to 3.0 mL of fresh trypsin-EDTA solution to cover the cell layer, and let it sit. 4. Incubate at 37°C for 2 to 7 minutes; monitor closely under an inverted microscope until the epithelial cell layer completely detaches, rounds up, and becomes mobile. 5. Add 6.0 to 8.0 mL of pre-warmed complete culture medium to the dish and gently pipette the cells up and down to achieve a uniform single-cell suspension. 6. Centrifuge at approximately 125 × g for 5 minutes, discard the supernatant, resuspend the cell pellet uniformly in fresh complete culture medium, and distribute the suspension into new vessels.
Medium Renewal	2 to 3 times per week
Subcultivation Ratio	Split optimal, confluent cultures at a standard ratio of 1:4 to 1:10. This line grows rapidly and tolerates high dilution steps easily.
Culture Conditions	Atmosphere: Air, 95%; CO2, 5%; Temperature: 37°C
Cryopreservation	90% Complete culture medium + 10% DMSO (or 50% Basal medium + 40% FBS + 10% DMSO)

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CSC-RR00935	Luciferase Reporter Cell Line - MDBK	Inquiry

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* For research use only. Not intended for any clinical use.

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