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| General Information | |
| Organism | Homo sapiens, human |
| Cell Line Description | Nthy-ori 3-1 is an immortalized human thyroid follicular epithelial cell line established by transfecting normal primary thyrocytes with an origin-defective SV40 genome. The cells retain selected differentiated thyroid functions, including iodide trapping and thyroglobulin production, while displaying growth-factor independence and low-frequency anchorage-independent growth. The original characterization found no tumor formation in nude mice. Because the line contains integrated SV40 large T antigen and is classified as transformed, it serves as an immortalized thyroid epithelial reference rather than a direct substitute for primary normal thyrocytes. Nthy-ori 3-1 is suitable for studies of thyroid growth control, differentiation, iodide handling, gene transfer, viral interactions, signaling, and comparative thyroid disease biology. |
| Cell Type | Human thyroid follicular epithelial cell |
| Tissue of Origin | Thyroid gland |
| Disease | No associated disease; derived from normal thyroid tissue before immortalization |
| Sex | Female |
| Age at Sampling | 35 years |
| Parent Cell Line | HTori-3 |
| Immortalization | Transfection with a plasmid containing an origin-defective SV40 genome (pSVori−), resulting in integration of SV40 large T antigen |
| Morphology | Epithelial |
| Growth Mode | Adherent |
| Product Format | Frozen |
| Containment Guidance | Handle at Containment Level 2 under the referenced handling framework. Perform a local risk assessment and follow applicable requirements for human-derived, genetically modified cell cultures. |
| Applications | 1. Human thyroid follicular epithelial biology 2. Thyroid growth control and cell-cycle studies 3. Thyroid differentiation, iodide uptake, and thyroglobulin biology 4. PAX8, thyroglobulin, thyroid peroxidase, and sodium/iodide symporter research 5. Gene-transfer and transfection studies 6. Virus–host interaction studies 7. Endocrine, metabolic, oxidative-stress, and signaling research 8. Comparison with thyroid tumor cell lines, with explicit recognition of SV40-mediated transformation 9. Transcriptomic, metabolomic, and genomic-variation studies |
| Characteristics | |
| Transformation Status | SV40-transformed, continuously proliferating thyroid follicular epithelial cell line with integrated large T antigen |
| Differentiated Thyroid Functions | Retains iodide-trapping activity and thyroglobulin production |
| Thyroid Marker and Functional Profile | Control Nthy-ori 3-1 cultures express PAX8, thyroglobulin, thyroid peroxidase, and the sodium/iodide symporter and demonstrate iodide uptake in the referenced functional study. |
| Growth-Factor Dependence | Growth-factor independent in the original characterization |
| Anchorage-Independent Growth | Capable of anchorage-independent growth at low frequency |
| Tumorigenicity | Non-tumorigenic in nude mice in the original characterization |
| Model Limitation | Nthy-ori 3-1 originates from normal thyroid tissue but is not a primary normal-cell model. SV40-mediated immortalization, integrated large T antigen, and long-term culture can alter growth regulation and experimental responses; primary thyrocytes or additional non-transformed models should be included when physiological normality is essential. |
| Culture Conditions and Handling | |
| Culture Conditions | 37°C in a humidified atmosphere containing 5% CO₂ |
| Passage Endpoint | Subculture at 70–80% confluence |
| Subcultivation Ratio | A split ratio of 1:3 to 1:6 is recommended |
| Recommended Seeding Density | 2-4 × 10⁴ cells/cm² |
| Dissociation Reagent | 0.05% trypsin or trypsin–EDTA |
| Subculturing | 1. Aspirate the spent medium from a 70–80% confluent culture. 2. Rinse the monolayer with a suitable balanced salt solution to remove residual serum. 3. Add enough 0.05% trypsin or trypsin–EDTA to cover the cell layer. 4. Monitor detachment and gently dislodge the cells as soon as the monolayer releases. 5. Add complete growth medium to stop enzyme activity, collect the cells, and prepare a uniform suspension. 6. Seed new vessels at 2–4 × 10⁴ cells/cm² or at a 1:3 to 1:6 split ratio. 7. Return the cultures to 37°C and 5% CO₂. |
| Handling Notes | Keep cultures below full confluence and standardize seeding density, passage timing, serum lot, and passage number across comparative experiments. Confirm identity against the appropriate STR reference and maintain routine contamination testing. Interpret results in the context of SV40 large T antigen expression, particularly for studies of proliferation, cell-cycle control, transformation, or normal thyroid physiology. |
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| CSC-RR00701 | Luciferase Reporter Cell Line - Nthy-ori 3-1 | Inquiry |
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