143B Cell Line

Brief Introduction
Product List

General Information
Organism	Homo sapiens, human
Cell Line Description	143B is a human malignant bone tumor cell line derived from the parental HOS (human osteosarcoma) cell line. It was originally established from a biopsy sample of a primary osteosarcoma lesion in a 13-year-old Caucasian female patient. A distinctive feature of this cell line is its thymidine kinase-negative (TK-) phenotype, obtained through 5-bromodeoxyuridine (BrdU) selection. 143B cells exhibit a mixed morphology—displaying both epithelioid and spindle-shaped characteristics—and grow as an adherent monolayer with a high proliferative capacity. In global cancer research, 143B serves as a preferred in vivo and in vitro model for studying aggressive osteosarcoma metastasis. Due to its high transfection efficiency and TK- baseline characteristics, it is a key host cell for somatic cell hybridization, mitochondrial DNA depletion experiments, and high-throughput non-viral transfection screening.
Tissue	Bone
Disease	Osteosarcoma
Morphology	Spindle and epithelial-like mixture
Gender	Female
Age	13 years
Product Format	Frozen
Growth Mode	Adherent Monolayer
Biosafety Level	1 (Biosafety classification is based on standard global cell repository criteria)
Applications	1. A flagship model for investigating the cellular mechanisms driving osteosarcoma growth and pulmonary metastasis; 2. A standard genetic host cell line suitable for gene delivery, non-viral vector design, and high-efficiency transfection; 3. Generation of mitochondrial rho^0 cell lines to elucidate the role of mitochondrial DNA in cellular respiration and energy transduction networks; 4. Preclinical screening for next-generation targeted osteosarcoma therapies, cisplatin resistance modulators, and natural killer (NK) cell immunotherapies; 5. Evaluation of cell-free DNA (cfDNA) extraction kits and mutation tracking mechanisms within liquid biopsy models.
Shipped In	Dry ice
Storage Temperature	−196°C (Liquid nitrogen vapor phase only)
Characteristics
Tumorigenic	Yes; exhibits high rapid tumorigenic kinetics and spontaneous metastatic capability in vivo.
Karyotype	Aneuploid / Hyperdiploid; displays highly unstable chromosomal numerical and structural deviations common to its parental HOS lineage. Trypsin-Giemsa banding confirms the lack of HeLa marker structures.
Genetic Profile	1. TP53 status: Mutant; harboring a homozygous simple point mutation: p.Arg156Pro (c.467G>C). 2. KRAS status: Hyperactivated; harboring concurrent heterozygous hotspot mutations: p.Gly12Ser (c.34G>A) and p.Ala59Thr (c.175G>A). 3. CDKN2A status: Homozygous gene deletion, resulting in complete loss of p16 expression. 4. Biochemical baseline: Thymidine kinase-deficient (TK-).
Microsatellite Status	Stable (MSS).
Growth Kinetics	Rapid adherent proliferation; average population doubling time is approximately 36 hours.
Mycoplasma Test	Negative.
Culture Conditions and Handling
Subculturing	1. Remove and discard the old complete growth medium. 2. Rinse the cell monolayer with sterile PBS, or perform a quick rinse with 0.25% trypsin-0.02% EDTA solution, to completely remove residual serum components. 3. Add fresh trypsin-EDTA solution (or Accutase) to cover the cells, and incubate the flask at room temperature or 37°C until the cells detach and become rounded (approximately 8 to 10 minutes). 4. Immediately add an equal volume of fresh complete growth medium to effectively stop the enzymatic reaction. 5. Gently pipette the cell suspension to mix thoroughly, then distribute it into new culture vessels. 6. Seeding guidelines: Ensure subculturing is performed before the cells reach full confluence (100% confluence). The recommended seeding density is 8.0 × 10^3 to 4.0 × 10^4 viable cells/cm².
Medium Renewal	2 to 3 times per week
Subcultivation Ratio	Split confluent cultures at a standard ratio of 1:4 to 1:8.
Culture Conditions	Atmosphere: Air, 95%; CO2, 5%; Temperature: 37°C
Cryopreservation	90% Complete growth medium + 10% DMSO (or 50% Basal medium + 40% FBS + 10% DMSO)

Cat.No.	Product Name	Price
CSC-RR0152	RFP Stable Cell Line-143B	Inquiry
CSC-RR00729	GFP Reporter Cell Line - 143B	Inquiry
CSC-RR00730	Luciferase Reporter Cell Line - 143B	Inquiry
CSC-RO01417	OVAL Stable Cell Line - 143B	Inquiry
CSC-RO01670	GFP/OVAL Stable Cell Line - 143B	Inquiry
CSC-RO01923	Luc/OVAL Stable Cell Line - 143B	Inquiry
CSC-RO02176	GFP/Luc/OVAL Stable Cell Line - 143B	Inquiry

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* For research use only. Not intended for any clinical use.

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