Our promise to you:
Guaranteed product quality, expert customer support.
24x7 CUSTOMER SERVICE
CONTACT US TO ORDER
| General Information | |
| Organism | Rattus norvegicus, rat |
| Cell Line Description | MADB106 is a homologous breast adenocarcinoma cell line derived from spontaneous lung metastases of breast tumors in Fischer 344 (F344) rats. It is one of the most widely recognized and used models for studying cancer metastasis biology, particularly "experimental metastasis" to the lungs. MADB106 cells exhibit an epithelial-like morphology and are known for their predictable and rapid colonization of the lungs after intravenous injection. This cell line is the preferred tool for studying the role of natural killer (NK) cells in controlling metastasis, assessing the effects of stress and anesthesia on tumor drug resistance, and testing the efficacy of immunomodulatory agents in immunocompetent mouse models. |
| Tissue | Mammary gland; derived from metastatic site (Lung) |
| Disease | Mammary Adenocarcinoma |
| Morphology | Epithelial-like |
| Gender | Female |
| Age | Adult |
| Product Format | Frozen |
| Growth Mode | Adherent |
| Biosafety Level | 1 (Biosafety classification is based on U.S. Public Health Service Guidelines) |
| Applications | 1. Research on the molecular mechanisms of lung metastasis 2. Assessment of host immunity, especially NK cell-mediated tumor clearance 3. Research on the effects of surgical stress and neuroendocrine factors on metastasis 4. Preclinical screening of anti-metastatic drugs and immunotherapeutic drugs 5. Construction of a F344 rat homologous metastasis model |
| Shipped In | Dry ice |
| Storage Temperature | −196°C |
| Characteristics | |
| Tumorigenic | Yes, highly tumorigenic and metastatic in syngeneic Fischer 344 (F344) rats |
| Organ Tropism | Exhibits high organ-specificity for pulmonary colonization |
| Growth Kinetics | Rapid growth rate in vitro; lung colonies become visible 14–21 days post-injection |
| Phenotype | Sensitive to NK cell-mediated lysis, making it a standard for NK-activity studies |
| Mycoplasma Test | Negative |
| Culture Conditions and Handling | |
| Subculturing | 1. Remove and discard the culture medium. 2. Quickly wash the cell layer with Ca²⁺/Mg²⁺-free PBS buffer to remove all serum residue. 3. Add 2.0 to 3.0 mL of 0.25% trypsin-0.53 mM EDTA solution and observe under an inverted microscope until the cell layer disperses (usually 3 to 7 minutes). 4. Add 6.0 to 8.0 mL of complete culture medium and gently pipette until a single-cell suspension is obtained. 5. Aliquot the cell suspension into new culture dishes. |
| Medium Renewal | 2 to 3 times per week |
| Subcultivation Ratio | A split ratio of 1:3 to 1:6 is recommended |
| Culture Conditions | Atmosphere: Air, 95%; CO2, 5%; Temperature: 37°C |
| Cryopreservation | Complete growth medium supplemented with 5% to 10% (v/v) DMSO |
| Cat.No. | Product Name | Price |
|---|---|---|
| CSC-RR00795 | Luciferase Reporter Cell Line - MADB106 | Inquiry |
| CSC-RO01510 | OVAL Stable Cell Line - MADB106 | Inquiry |
| CSC-RO01763 | GFP/OVAL Stable Cell Line - MADB106 | Inquiry |
| CSC-RO02016 | Luc/OVAL Stable Cell Line - MADB106 | Inquiry |
| CSC-RO02269 | GFP/Luc/OVAL Stable Cell Line - MADB106 | Inquiry |
The above is only part of a part of cell line products. If you don't find the cell line you want, Creative Biogene can also provide stable cell line generation service with the best prices and fastest turnaround time for you! Contact us for more information or to request a quote.
Copyright © 2026 Creative Biogene. All rights reserved.