VcaP Cell Line

Brief Introduction
Product List

General Information
Organism	Homo sapiens, human
Cell Line Description	VCaP is a human prostate cancer cell line established by K.J. Pienta and colleagues in 1997. The cell line was derived from a 59-year-old Caucasian male with advanced, hormone-refractory prostate cancer; the sample was obtained from a metastatic lesion in the lumbar spine during autopsy. The harvested tissue was initially propagated as a xenograft in SCID mice before being established as an in vitro cell line. VCaP cells exhibit an epithelioid morphology, growing as flat, tightly packed adherent cell islands accompanied by scattered clusters of floating cells. In the field of translational oncology, VCaP is regarded as a highly valuable and pivotal physiological model. It is widely recognized as the only native prostate cancer cell model capable of simultaneously expressing the TMPRSS2-ERG gene fusion and androgen receptor splice variant 7 (AR-V7), making it a cornerstone tool for modeling advanced castration-resistant prostate cancer (CRPC).
Tissue	Prostate
Disease	Prostate Carcinoma / Castration-Resistant Prostate Cancer (CRPC)
Morphology	Epithelial-like; grows in small, tightly formed clusters and flattened clonal islands
Gender	Male
Age	59 years
Product Format	Frozen
Growth Mode	Adherent
Biosafety Level	2 (BSL-2 is standardly enforced; during its historical mouse xenograft passaging, the line acquired an integrated xenotropic murine leukemia virus-related virus, XMRV/Bxv-1, and secretes viral particles)
Applications	1. An indispensable in vitro model for studying the structural basis of oncogenesis driven by the TMPRSS2-ERG chromosomal translocation; 2. Investigating the mechanisms underlying castration resistance, androgen-independent growth, and AR-V7 splice variant signaling; 3. Preclinical validation of next-generation androgen receptor antagonists (e.g., enzalutamide) and targeted therapies; 4. Elucidating the dynamics of organ-specific bone tropism and osteolytic/osteoblastic bone metastasis in prostate cancer; 5. Establishing subcutaneous or orthotopic tumor xenograft models in immunodeficient mice that exhibit slow growth and closely recapitulate clinical pathological features.
Shipped In	Dry ice
Storage Temperature	−196°C
Characteristics
Tumorigenic	Yes; highly tumorigenic in immunocompromised mice, mirroring human metastatic skeletal presentation.
Oncogenic Drivers	1. TMPRSS2-ERG fusion: A hallmark gene fusion associated with prostate cancer. 2. AR-V7 splice variant: Constitutive expression of a truncated androgen receptor that remains active in a ligand-independent manner.
Expression Profile	Natively synthesizes copious quantities of Prostate-Specific Antigen (PSA), Prostatic Acid Phosphatase (PAP), Cytokeratin-18, and wild-type Androgen Receptors (AR).
Mycoplasma Test	Negative
Culture Conditions and Handling
Subculturing	1. Do not discard the floating cells. These clusters of floating cells contain a significant number of viable cells in the mitotic phase. 2. Collect the spent medium containing the floating cell clusters and transfer it to a sterile conical centrifuge tube. Centrifuge at a low speed (approximately 125 × g) for 5 minutes to pellet the cells. 3. Wash the remaining adherent monolayer cells with sterile PBS (free of Ca2+ and Mg2+). 4. Note on trypsin sensitivity: VCaP cells are highly sensitive to damage caused by trypsin. It is strongly recommended to use Accutase instead of trypsin. Add 1.0 to 2.5 mL of Accutase and incubate at room temperature or 37°C for 8 to 10 minutes until the cells detach. Do not assist detachment by tapping or shaking the culture flask. 5. Resuspend the detached cells in complete medium and combine them with the cell pellet collected from the spent medium. 6. Centrifuge the combined cell suspension at low speed, resuspend in a mixture of media (e.g., 90% fresh medium + 10% sterile-filtered spent medium), and seed into a new culture vessel.
Culture Conditions	Atmosphere: Air, 95%; CO2, 5%; Temperature: 37°C
Cryopreservation	90% Complete culture growth medium + 10% DMSO (or 50% Basal medium + 40% FBS + 10% DMSO)

Cat.No.	Product Name	Price
CSC-RR0355	GFP Reporter Cell Line - VcaP	Inquiry
CSC-RO01665	OVAL Stable Cell Line - VcaP	Inquiry
CSC-RO01918	GFP/OVAL Stable Cell Line - VcaP	Inquiry
CSC-RO02171	Luc/OVAL Stable Cell Line - VcaP	Inquiry
CSC-RO02424	GFP/Luc/OVAL Stable Cell Line - VcaP	Inquiry
CSC-RR00957	Luciferase Reporter Cell Line - VCAP	Inquiry
CSC-RR01015	GFP/Luc Reporter Cell Line - VCaP	Inquiry

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* For research use only. Not intended for any clinical use.

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