NCI-H2030 Cell Line

Brief Introduction
Product List

General Information
Organism	Homo sapiens, human
Cell Line Description	NCI-H2030 is a human lung adenocarcinoma cell line derived from a male nonsmoker with non-small cell lung cancer. The cells grow as an adherent, epithelial-like monolayer and carry homozygous KRAS p.Gly12Cys and TP53 p.Gly262Val variants with a microsatellite-stable background. This combination supports studies of KRAS G12C signaling and pharmacology, TP53-altered lung cancer, metastatic disease, pathway dependency, resistance mechanisms, biomarker development, and multi-omics.
Cell Type	Human lung adenocarcinoma epithelial cell
Tissue	Lung
Disease	Lung adenocarcinoma; non-small cell lung cancer
Year Established	1988
Gender	Male
Smoking History	Nonsmoker
Morphology	Epithelial-like; adherent cells are predominantly polygonal with distinct intercellular boundaries
Growth Mode	Adherent monolayer
Culture Type	Continuous cancer cell line
Applications	1. Lung adenocarcinoma and non-small cell lung cancer research 2. Metastatic lung cancer biology 3. KRAS G12C signaling and pathway-dependency studies 4. Covalent KRAS G12C inhibitor evaluation 5. Adaptive response and acquired-resistance research 6. MAPK and PI3K pathway analysis 7. TP53-altered tumor biology 8. Biomarker discovery and validation 9. Drug combination and cytotoxicity assays 10. CRISPR, RNA interference, and gene-expression studies 11. Transcriptomic, genomic, proteomic, and pharmacogenomic analyses 12. Two-dimensional and three-dimensional tumor models
Characteristics
KRAS Variant	KRAS c.34G>T, producing p.Gly12Cys (G12C)
TP53 Variant	TP53 c.785G>T, producing p.Gly262Val
Microsatellite Status	Microsatellite stable (MSS)
Mycoplasma Test	Negative
Culture Conditions and Handling
Culture Conditions	37°C in a humidified atmosphere containing 5% CO₂ in air
Culture Surface	Standard tissue-culture-treated vessels; routine propagation does not require a coating
Medium Renewal	Every 2–3 days
Dissociation Reagent	0.25% trypsin with 0.53 mM EDTA
Subculturing	1. Remove spent medium. 2. Rinse the monolayer briefly with 0.25% trypsin–0.53 mM EDTA and remove the rinse. 3. Add 1–2 mL fresh trypsin–EDTA to the culture vessel. 4. Incubate at room temperature or 37°C until cells detach. 5. Add complete medium and disperse the cells gently. 6. Transfer the required fraction to fresh vessels containing pre-warmed complete medium. 7. Return cultures to 37°C and 5% CO₂ and confirm even attachment and expected epithelial morphology.
Recommended Split Ratio	1:3 to 1:4
Recovery pH Control	Pre-equilibrate complete medium in the incubator for at least 15 minutes before seeding thawed cells. Maintain the medium near pH 7.0–7.6 and minimize alkaline exposure during recovery.
Thawing	Thaw rapidly with gentle agitation in a 37°C water bath for approximately 2 minutes. Disinfect the vial exterior, transfer the contents into 9 mL complete medium, and process under aseptic conditions.
Cryopreservation Medium	Complete growth medium supplemented with 5% DMSO
Long-Term Storage	Below −130°C, preferably in the vapor phase of liquid nitrogen; −70°C storage is not suitable for continued preservation
Biosafety	Handle as human-derived research material under Biosafety Level 1 or the locally assigned equivalent. Apply site-specific risk assessment, aseptic technique, appropriate personal protective equipment, decontamination, and waste procedures.
Handling Notes	Record passage number, confluence, split ratio, dissociation exposure, medium and serum lot, feeding schedule, and time after seeding. Avoid over-trypsinization and prolonged overconfluence, and distinguish parental cells from labeled or selected derivatives in all records.

Cat.No.	Product Name	Price
CSC-RR01017	GFP/Luc Reporter Cell Line - NCI-H2030	Inquiry

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