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| General Information | |
| Organism | Canis lupus familiaris, dog |
| Cell Line Description | MDCK (Madin-Darby canine kidney cells) is a mammalian epithelial cell line established in 1958 from the kidney tissue of normal adult female Cocker Spaniels. It is one of the most widely used and extensively studied model systems in cell biology. MDCK cells exhibit a distinct epithelial cell morphology; when cultured to confluence on permeable supports, they form a highly polarized and functionally complete monolayer of cells with clearly defined apical and basal regions interconnected by tight junctions. This cell line is the gold standard for studying epithelial cell polarity, protein transport, and membrane transport. Furthermore, due to their high susceptibility to various mammalian and avian viruses, MDCK cells are also an important substrate for influenza virus research and vaccine production. |
| Tissue | Kidney |
| Disease | Normal |
| Morphology | Epithelial |
| Gender | Female |
| Age | Adult |
| Product Format | Frozen |
| Growth Mode | Adherent |
| Biosafety Level | 1 (Biosafety classification is based on U.S. Public Health Service Guidelines) |
| Applications | 1. Studies on epithelial cell polarity and junctional complex assembly 2. Viral replication studies and industrial vaccine production (especially influenza vaccines) 3. Studies on membrane protein transport and sorting mechanisms 4. Drug permeability assays and renal transport studies 5. In vitro simulations of epithelial barrier function and cyst formation |
| Shipped In | Dry ice |
| Storage Temperature | −196°C |
| Characteristics | |
| Tumorigenic | Low; can form tumors in nude mice under specific conditions or after transformation |
| Karyotype | Heteroploid; modal number = 78 (range 70–84) |
| Polarity | High; develops high Trans-Epithelial Electrical Resistance (TEER) |
| Phenotype | Retains properties of distal tubule or collecting duct epithelium |
| Mycoplasma Test | Negative |
| Culture Conditions and Handling | |
| Subculturing | 1. Remove and discard the culture medium. 2. Quickly wash the cell layer with Ca²⁺/Mg²⁺-free PBS buffer to remove all serum residue. 3. Add 2.0 to 3.0 mL of 0.25% trypsin-0.53 mM EDTA solution and observe under an inverted microscope until the cell layer disperses (usually 5 to 10 minutes). 4. Add complete culture medium to neutralize the trypsin and gently pipette to obtain a single-cell suspension. 5. Aliquot the cell suspension into new culture dishes. |
| Medium Renewal | 2 to 3 times per week |
| Subcultivation Ratio | A split ratio of 1:4 to 1:10 is recommended |
| Culture Conditions | Atmosphere: Air, 95%; CO2, 5%; Temperature: 37°C |
| Cryopreservation | Complete growth medium supplemented with 5% to 10% (v/v) DMSO |
| Cat.No. | Product Name | Price |
|---|---|---|
| CSC-RR0085 | tGFP Reporter Stable Cell Line-MDCK | Inquiry |
| CSC-SC016312-1 | Human TMPRSS11E Stable Cell Line - MDCK | Inquiry |
| CSC-RO01314 | Human ABCC3 Stable Cell Line - MDCK | Inquiry |
| CSC-RO02626 | Dog ABCB1 Stable Cell Line - MDCK | Inquiry |
| CSC-RO02627 | Dog ABCB1(aa1-91) Stable Cell Line - MDCK | Inquiry |
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