MPC-5 Cell Line

Brief Introduction
Product List

General Information
Organism	Mus musculus, mouse
Cell Line Description	MPC-5 is a conditionally immortalized mouse podocyte cell line established by P. Mundel and colleagues in 1997. This cell line was isolated from the kidneys of H2Kb-tsA58 transgenic mice (the "Immortomouse" background) carrying the temperature-sensitive simian virus 40 large T antigen (SV40LT), which is controlled by an interferon-γ (IFN-γ) inducible promoter. This unique genetic system allows MPC-5 to switch between a proliferating undifferentiated state and a non-proliferating mature podocyte state. Due to this dual nature, MPC-5 has become the global gold standard in vitro model for studying glomerular visceral epithelial biology, podocyte differentiation, slit membrane molecular dynamics, and the mechanisms of damage associated with diabetic nephropathy and focal segmental glomerulosclerosis (FSGS).
Tissue	Kidney; Glomerulus
Disease	Normal
Cell Type	Podocyte (Glomerular Visceral Epithelial Cell)
Morphology	Epithelial-like / Cobblestone (at 33°C); Arborized / Spreading with extensive processes (at 37°C)
Gender	Not specified
Age	10 weeks
Product Format	Frozen
Growth Mode	Adherent
Biosafety Level	1 (Contains a non-infectious, integrated temperature-sensitive SV40 large T antigen)
Applications	1. Investigate podocyte differentiation, cytoskeletal structure, and foot process formation. 2. Construct diabetic nephropathy and oxidative stress models using a high glucose exposure model. 3. Evaluate the mechanisms of proteinuria, slit membrane damage, and focal segmental glomerulosclerosis. 4. Screen for renal protective compounds and podocyte apoptosis inhibitors. 5. Investigate intercellular signaling within the glomerular filtration barrier.
Shipped In	Dry ice
Storage Temperature	−196°C
Characteristics
Tumorigenic	No, non-tumorigenic under restrictive differentiation conditions
Immortalization	Conditionally immortalized via a temperature-sensitive tsA58 SV40 Large T antigen transgene
Growth States	1. Permissive culture (33°C + IFN-γ): Rapid proliferation, pebble-like morphology, SV40LT active expression. 2. Restrictive culture (37°C, without IFN-γ): Growth arrest, extensive dendritic/foot process expansion, SV40LT degradation.
Expression Markers	WT1 (Wilms' Tumor 1) was constitutively expressed in both states; synaptoprotein, renin, podocyte protein and CD2AP were significantly upregulated 10-14 days after differentiation at 37°C.
Mycoplasma Test	Negative
Culture Conditions and Handling
Subculturing (Expansion Only)	1. Remove and discard the culture supernatant. 2. Gently wash the cell monolayer with Ca²⁺/Mg²⁺-free PBS. 3. Add 0.25% trypsin-0.53 mM EDTA solution and incubate at 33°C (usually 3 to 6 minutes) until cells detach. 4. Neutralize the trypsin with complete culture medium; gently pipette to obtain a single-cell suspension. 5. Transfer the cells to a new culture dish. Note: Subculturing for maintenance and expansion of cells must be performed at 33°C and with the addition of IFN-γ.
Differentiation Induction	To initiate the differentiation process for subsequent experiments, cells were collected at 33°C, resuspended in complete medium completely free of IFN-γ, and seeded onto experimental culture plates. Cells were strictly cultured at 37°C for 10 to 14 days without subculturing until a mature differentiation phenotype was formed.
Subcultivation Ratio	A split ratio of 1:3 to 1:6 is recommended (during the 33°C expansion phase)
Culture Conditions	Atmosphere: Air, 95%; CO2, 5%; Temperature: 33°C for growth, 37°C for differentiation.
Cryopreservation	90% complete culture medium (containing IFN-γ) + 10% DMSO

Cat.No.	Product Name	Price
CSC-RO01391	Mouse Tollip Stable Cell Line - MPC-5	Inquiry
CSC-RO02574	Cas9 Stable Cell Line - MPC-5	Inquiry
CSC-RR00876	GFP Reporter Cell Line - MPC-5	Inquiry
CSC-RR00925	Luciferase Reporter Cell Line - MPC-5	Inquiry

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