Hut-78 Cell Line

Brief Introduction
Product List

General Information
Organism	Homo sapiens, human
Cell Line Description	HuT 78 is a human T-lymphoblastoid cell line established in 1980 by Adi F. Gazdar, John D. Minna, and their colleagues at the National Cancer Institute (NCI). The cell line was isolated from the peripheral blood of a 53-year-old white male patient diagnosed with Sézary syndrome—a highly aggressive, leukemic variant of cutaneous T-cell lymphoma (CTCL). HuT 78 cells grow exclusively in suspension, appearing morphologically as round, single cells or loose multicellular clusters. HuT 78 is a globally renowned model cell line in the fields of immunology, virology, and hematology. It retains the functional characteristics of mature helper/inducer T cells (CD4+ phenotype) and actively secretes interleukin-2 (IL-2). This cell line has played a pivotal role in the history of HIV research; the famous H9 clone—used by Robert Gallo's laboratory to isolate and propagate HIV-1/HTLV-III—was derived from HuT 78 as a selected, engineered single-cell clone highly susceptible to viral replication.
Tissue	Blood / Lymphoid; derived from peripheral blood
Cell Type	T Lymphoblast (helper/inducer T-cell)
Ethnicity	Caucasian
Disease	Sézary Syndrome (Cutaneous T-Cell Lymphoma)
Morphology	Lymphoblast-like; round cells
Gender	Male
Age	53 years
Product Format	Frozen
Growth Mode	Suspension
Biosafety Level	1 (Biosafety classification matches standard laboratory safety parameters for non-infectious human lymphoid cells)
Applications	1. Gold-standard in vitro model for studying T-cell receptor (TCR) activation pathways, mature T-helper cell biology, and immune-oncology signaling; 2. Direct modeling of Cutaneous T-Cell Lymphoma (CTCL) and Sézary syndrome pathogenesis and therapeutic responses; 3. Host system for investigating the replication kinetics, entry mechanisms, and cellular restrictions of human immunodeficiency virus (HIV-1); 4. Dissecting interleukin-2 (IL-2) autocrine/paracrine signaling loops and T-cell survival cascades; 5. Preclinical high-throughput screening for novel small-molecule chemotherapeutics and targeted immunotherapies for T-cell lymphomas.
Shipped In	Dry ice
Storage Temperature	−196°C
Characteristics
Tumorigenic	Yes; forms progressively expanding T-cell lymphoma tumors in highly immunocompromised host models.
Transformant	None; spontaneously immortalized from the malignant cells of an advanced clinical donor.
Antigen & Receptor Expression	Expresses a mature T-helper phenotype: CD4 positive, CD3 positive, and CD8 negative. Natively expresses high levels of the Interleukin-2 receptor (IL-2R).
Secretory Activity	It synthesizes and secretes biologically active interleukin-2 (IL-2). Exogenous IL-2 promotes the growth of HuT 78 cells, whereas tumor necrosis factor-alpha (TNF-α) functions as an autocrine growth factor for this cell line.
Mycoplasma Test	Negative
Culture Conditions and Handling
Subculturing	1. Since these cells grow entirely in suspension, trypsinization is not required. 2. Gently shake or swirl the culture vessel to create a uniform cell suspension. 3. Transfer the culture medium containing the suspended cells to a sterile conical centrifuge tube. 4. Centrifuge the suspension at approximately 125 × g (or 1000 rpm) for 5 to 7 minutes. 5. Carefully aspirate or decant the supernatant, ensuring the cell pellet remains intact. 6. Gently resuspend the cell pellet using pre-warmed, fresh complete growth medium.
Thawing Protocol Note	During the early recovery phase following thawing, HuT 78 cells are highly sensitive to drastic pH fluctuations and alkaline environmental conditions. Before adding the thawed cell suspension, the culture vessel containing fresh complete growth medium should be placed in a 5% CO2 incubator for at least 15 minutes to allow the medium to reach and equilibrate within the normal physiological pH range (7.0 to 7.6).
Medium Renewal	2 to 3 times per week (or complete fluid change depending on metabolic indicators/medium acidification)
Subcultivation Ratio	Split confluent suspension cultures at a standard ratio of 1:2 to 1:4.
Culture Conditions	Atmosphere: Air, 95%; CO2, 5%; Temperature: 37°C
Cryopreservation	95% Complete culture growth medium + 5% DMSO (or standard 90% Complete culture growth medium + 10% DMSO)

Cat.No.	Product Name	Price
CSC-RR0628	Luciferase Reporter Cell Line - Hut 78	Inquiry
CSC-RR00642	GFP Reporter Cell Line - HuT 78	Inquiry
CSC-RO01602	OVAL Stable Cell Line - Hut-78	Inquiry
CSC-RO01855	GFP/OVAL Stable Cell Line - Hut-78	Inquiry
CSC-RO02108	Luc/OVAL Stable Cell Line - Hut-78	Inquiry
CSC-RO02361	GFP/Luc/OVAL Stable Cell Line - Hut-78	Inquiry

The above is only part of a part of cell line products. If you don't find the cell line you want, Creative Biogene can also provide stable cell line generation service with the best prices and fastest turnaround time for you! Contact us for more information or to request a quote.

* For research use only. Not intended for any clinical use.

Quick Inquiry